Sorting of precursor proteins between isolated spinach leaf mitochondria and chloroplasts
Tóm tắt
The precursors of the F1-ATPase β-subunits fromNicotiana plumbaginifolia andNeurospora crassa were imported into isolated spinach (Spinacia oleracea L.) leaf mitochondria. Both F1β precursors were imported and processed to mature size products. No import of the mitochondrial precursor proteins into isolated intact spinach chloroplasts was seen. Moreover, the precursor of the 33 kDa protein of photosynthetic water-splitting enzyme was not imported into the leaf mitochondria. This study provides the first experimental report ofin vitro import of precursor proteins into plant mitochondria isolated from photosynthetic tissue and enables studies of protein sorting between mitochondria and chloroplasts in a system which is homologous with respect to organelles. The results suggest a high organellar specificity in the plant cell for the cytoplasmically synthesized precursor proteins.
Tài liệu tham khảo
Andersson B, Åkerlund H-E: Proteins of the oxygen-evolving complex. In: Barber J (ed) Topics in Photosynthesis, Vol. 8, pp. 379–429. Elsevier, Amsterdam (1987).
Andersson B, Herrmann RG: Structure, function and biogenesis of nuclear-encoded proteins of photosystem II. In: Harwood JL, Walton TJ (eds) ‘Plant Membranes—Structure, Assembly and Function’, pp. 33–45. The Biochemical Society, London (1988).
Boutry M, Chua NH: A nuclear gene encoding the beta subunit of the mitochondrial ATP-synthase inNicotiana plumbaginifolia. EMBO J 5: 2159–2165 (1985).
Boutry M, Ferenc N, Chua NH: The beta subunit of a plant mitochondrial ATP synthase has a presequence involved in mitochondrial targeting. In: von Wettstein J, Chua NH (eds) Plant Molecular Biology, NATO Series 140: 127–134 (1987).
Boutry M, Nagy F, Poulsen C, Aoyagi K, Chua NH: Targeting of bacterial chloramphenicol acetyltransferase to mitochondria in transgenic plants. Nature 328: 340–342 (1987).
Cioppa GD, Kishore GM, Beachy RN, Fraley RT: Protein trafficking in plant cells. Plant Physiol 84: 965–968 (1987).
Franzén L-G, Frank G, Zuber H, Rochaix J-D: Chloroplast transit peptides from the green alga Chlamydomonas reinhardtii share features with both mitochondrial and higher plant chloroplast presequences. Mol Gen Genet, in press.
Gray MW: Organelle origins and ribosomal RNA. Biochem Cell Biol 66: 325–346 (1988).
Hamasur B, Birgersson U, Eriksson AC, Glaser E: Large scale purification procedure of spinach leaf mitochondria. Physiol Plant, in press.
Hartl FU, Pfanner N, Nicholson DW, Neupert W: Mitochondrial protein import. Biochim Biophys Acta 988: 1–45 (1989).
Heijne GV, Steppuhn J, Herrmann RG: Domain structure of mitochondrial and chloroplast targeting peptides. Eur J Biochem 180: 535–545 (1989).
Hurt EC, Soltanifar N, Clermont MG, Rochaix SD, Schatz G: The cleavable presequence of an imported chloroplast protein directs attached polypeptides into yeast mitochondria. EMBO J 5: 1343–1350 (1986).
Kirwin PM, Meadow JW, Shachleten B, Musgrove J-E, Eldenfild PP, Mould R, Hay NA, Robinson C: ATP-dependent import of a lumental protein by isolated thylakoid vesicles. EMBO J 8: 2251–2255 (1989).
Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685 (1970).
Leaver CJ, Gray MW: Mitochondrial genome organization and expression in higher plants. Annu Rev Plant Physiol 33: 273–308 (1982).
LevingsIII CS, Brown GG: Molecular biology of plant mitochondria. Cell 56: 171–179 (1989).
Lubben TH, Gatenby AA, Ahlquist P, Keegstra K: Chloroplast import characteristics of chimeric proteins. Plant Mol Biol 12: 13–18 (1989).
Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1982).
Melton DA, Krieg PA, Rebagliati MR, Maniatis T, Zinn K, Green MR: Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promotor. Nucl. Acids Res. 12: 7035–7056 (1984).
Nielsen DA, Shapiro DJ: Preparation of capped RNA transcripts using T7 RNA polymerase. Nucl Acids Res 25: 5936–5941 (1986).
Pelham HRB, Jackson RJ: An efficient mRNA-dependent translation system from reticulocyte lysate. Eur J Biochem 67: 247–256 (1976).
Pfaller R, Freitag H, Harmey MA, Benz R, Neupert W: A water-soluble form of porin from mitochondrial outer membrane ofNeurospora crassa. J Biol Chem 260: 8188–8193 (1985).
Pfaller R, Pfanner N, Neupert W: Byass of proteinaceous surface receptors can occur with low specificity and efficiency. J. Biol Chem 264: 34–39 (1989).
Quetier F, Lejeune B, Delorme S, Falconet D: Plant mitochondrial genome. In: Douce R, Day DA (eds) ‘Higher Plant Cell Respiration’, pp. 25–36. Encyclopedia of Plant Physiology, vol. 18. Springer-Verlag, Berlin (1985).
Robinson C, Ellis J: Partial purification of a chloroplast protease involved in the processing of imported precursor polypeptides. Eur J Biochem 142: 337–342 (1984).
Schmidt GW, Mishkind ML: Transport of proteins into chloroplasts. Annu Rev Biochem 55: 879–912 (1986).
Schmitz UK, Lonsdale DM: A yeast mitochondrial presequence functions as a signal for targeting to plant mitochondria in vivo. Plant Cell 1: 783–791 (1989).
Shinozaki K, Ohme M, Tanaka M, Wakasugu T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kako A, Tondon N, Shimada H, Sugiura J: The complete nucleotide sequence of tobacco chloroplast genome: its gene organization and expression. EMBO J 5: 2043–2049 (1986).
Smeekens S, Steeg HV, Bauerle C, Bettenbroek H, Keegstra K, Weisbeek P: Import into chloroplasts of a yeast mitochondrial protein directed by ferredoxin and plastocyanin transit peptides. Plant Mol Biol 9: 377–388 (1987).
Verner K, Schatz G: Import of an incompletely folded precursor protein into isolated mitochondria requires an energized inner membrane, but no added ATP. EMBO J 6: 2449–2456 (1987).
Westhoff P, Jansson C, Hitpab LK, Berzborn R, Larsson C, Barlett SG: Intracellular coding sites of polypeptides associated with photosynthetic oxygen evolution of photosystem II. Plant Mol Biol 4: 137–143 (1985).
Whelan J, Dolan L, Harmey MA: Import of precursor proteins intoVicia faba mitochondria. FEBS Lett 236: 217–220 (1988).
White JA, Scandalios JG: Isolation and characterization of a cDNA for mitochondrial manganese superoxidase dismutase (SOD-3) of maize and its relation to other manganese superoxide dismutases. Biochim Biophys Acta 951: 61–70 (1988).