Sonication and grinding pre-treatments on Gelidium amansii seaweed for the extraction and characterization of Agarose

Kit Wayne Chew1,2, Pau Loke Show1,2, Yee Jiun Yap2,3, Joon Ching Juan4, Siew Moi Phang5,6, Tau Chuan Ling6, Jo-Shu Chang7
1Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih, Malaysia
2Bioseparation Research Group, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih, Malaysia
3Department of Applied Mathematics, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih, Malaysia
4Nanotechnology & Catalysis Research Centre (NANOCAT), Institute of Postgraduate Studies, University of Malaya, Kuala Lumpur, Malaysia
5Institute of Ocean and Earth Sciences, University of Malaya, Kuala Lumpur, Malaysia
6Institute of Biological Sciences, University of Malaya, Kuala Lumpur, Malaysia
7Department of Chemical Engineering, National Cheng Kung University, Tainan, China

Tóm tắt

Various pretreatments methods including sonication and grinding were performed on red seaweed Gelidium amansii for the subsequent extraction of agarose. The agarose products are usually extracted from agar powder products from seaweeds. In this study, the agarose was extracted using a direct polyethylene glycol (PEG) method without the need to first process the agar from seaweed. The agar extract was frozen then thawed and mixed directly with PEG solution to precipitate the agarose. The quality of agarose obtained was evaluated through physico-chemical properties analysis which includes spectral technique (FTIR), melting and boiling point, gel strength and sulfate content. These properties were compared with a non-pretreated sample and it was found that the addition of pretreatment steps improved the quality of agarose but gave a slightly lower yield. The gel strength of pretreated samples was much higher and the sulfate content was lower compared to non-pretreated samples. The best pretreatment method was sonication which gave gel strength of 742 g cm-2 and sulfate content of 0.63%. The extraction of agarose can be further improved with the use of different neutralizing agents. Pretreating the seaweed shows potential in improving the quality of agarose from seaweed and can be applied for future extraction of the agarose.

Tài liệu tham khảo

Al-Nahdi Z, Al-Alawi A, Al-Marhobi I, Al-Zefiti A (2015). Optimization of yield and chemical properties of agar extracted from Melanothamnus somalensis from Oman sea. Journal of Environmental Science & Engineering, 4: 302–314 Alfred P (1967). Fractionation of mixtures of agarose and agaropectin. Ed: Google Patents Araki C (1966). Some recent studies on the polysaccharides of agarophytes. In: Proceedings of the International Seaweed Symposium, 3–17 Arvizu-Higuera D L, Rodríguez-Montesinos Y E, Murillo-Álvarez J I, Muñoz-Ochoa M, Hernández-Carmona G (2008). Effect of alkali treatment time and extraction time on agar from Gracilaria vermiculophylla. Journal of Applied Phycology, 20(5): 515–519 Azizi N, Najafpour G, Younesi H (2017). Acid pretreatment and enzymatic saccharification of brown seaweed for polyhydroxybutyrate (PHB) production using Cupriavidus necator. International Journal of Biological Macromolecules, 101: 1029–1040 Chemat F, Rombaut N, Sicaire A G, Meullemiestre A, Fabiano-Tixier A S, Abert-Vian M (2017). Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications: A review. Ultrasonics Sonochemistry, 34: 540–560 Chew K W, Juan J C, Phang S M, Ling T C, Show P L (2018). An overview on the development of conventional and alternative extractive methods for the purification of agarose from seaweed. Separation Science and Technology, 53(3): 467–480 Chirapart A, Praiboon J (2018). Comparison of the photosynthetic efficiency, agar yield, and properties of Gracilaria salicornia (Gracilariales, Rhodophyta) with and without adelphoparasite. Journal of Applied Phycology, 30(1): 149–157 Cole K M, Sheath R G (1990). Biology of the Red Algae. Oxford: Cambridge University Press Davies L A, Dargue A, Dean J R, Deary M E (2015). Use of 24 kHz ultrasound to improve sulfate precipitation from wastewater. Ultrasonics Sonochemistry, 23: 424–431 Dodds J, Espitalier F, Louisnard O, Grossier R, David R, Hassoun M, Baillon F, Gatumel C, Lyczko N (2007). The Effect of ultrasound on crystallisation–precipitation processes: Some examples and a new segregation model. Particle & Particle Systems Characterization, 24 (1): 18–28 Dodgson K S (1961). Determination of inorganic sulphate in studies on the enzymic and non-enzymic hydrolysis of carbohydrate and other sulphate esters. Biochemical Journal, 78(2): 312–319 Duckworth M, Yaphe W (1971). Preparation of agarose by fractionation from the spectrum of polysaccharides in agar. Analytical Biochemistry, 44(2): 636–641 Fidelis G P, Camara R B G, Queiroz M F, Santos Pereira Costa M S, Santos P C, Rocha H A O, Costa L S (2014). Proteolysis, NaOH and ultrasound-enhanced extraction of anticoagulant and antioxidant sulfated polysaccharides from the edible seaweed, Gracilaria birdiae. Molecules (Basel, Switzerland), 19(11): 18511–18526 Freile-Pelegrín Y, Murano E (2005). Agars from three species of Gracilaria (Rhodophyta) from Yucatán Peninsula. Bioresource Technology, 96(3): 295–302 Guerrero P, Etxabide A, Leceta I, Peñalba M, de la Caba K (2014). Extraction of agar from Gelidium sesquipedale (Rhodopyta) and surface characterization of agar based films. Carbohydrate Polymers, 99: 491–498 Hjertén S (1962). A new method for preparation of agaraose for gel electrophoresis. Biochimica et Biophysica Acta, 62(3): 445–449 Hu Y, Wang S, Wang Q, He Z, Lin X, Xu S, Ji H, Li Y (2016). Effect of different pretreatments on the thermal degradation of seaweed biomass. Proceedings of the Combustion Institute, 36(2): 2271–2281 Hurtado M Á, Manzano-Sarabia M, Hernández-Garibay E, Pacheco-Ruíz I, Zertuche-González J A (2011). Latitudinal variations of the yield and quality of agar from Gelidium robustum (Gelidiales, Rhodophyta) from the main commercial harvest beds along the western coast of the Baja California Peninsula, Mexico. Journal of Applied Phycology, 23(4): 727–734 Jeon Y J, Athukorala Y, Lee J H (2005). Characterization of agarose product from agar using DMSO. Algae-Korean Phycological Society, 20(1): 61–67 Kolanthai E, Ganesan K, Epple M, Kalkura S N (2016). Synthesis of nanosized hydroxyapatite/agarose powders for bone filler and drug delivery application. Materials Today Communications, 8: 31–40 Lee P Y, Costumbrado J, Hsu C Y, Kim Y H (2012). Agarose gel electrophoresis for the separation of DNA fragments. Journal of Visualized Experiments, 62(62): 3923 Matsuhashi T (1977). Acid pretreatment of agarophytes provides improvement in agar extraction. Journal of Food Science, 42(5): 1396–1400 Meena R, Prasad K, Siddhanta A (2011). Preparation of superior quality products from two Indian agarophytes. Journal of Applied Phycology, 23(2): 183–189 Meena R, Siddhanta A, Prasad K, Ramavat B, Eswaran K, Thiruppathi S, Ganesan M, Mantri V A, Rao P S (2007). Preparation, characterization and benchmarking of agarose from Gracilaria dura of Indian waters. Carbohydrate Polymers, 69(1): 179–188 Mittal R, Tavanandi H A, Mantri V A, Raghavarao K S M S (2017). Ultrasound assisted methods for enhanced extraction of phycobiliproteins from marine macro-algae, Gelidium pusillum (Rhodophyta). Ultrasonics Sonochemistry, 38: 92–103 Montingelli M, Benyounis K, Quilty B, Stokes J, Olabi A (2017). Influence of mechanical pretreatment and organic concentration of Irish brown seaweed for methane production. Energy, 118: 1079–1089 Pereira-Pacheco F, Robledo D, Rodríguez-Carvajal L, Freile-Pelegrín Y (2007). Optimization of native agar extraction from Hydropuntia cornea from Yucatán, México. Bioresource Technology, 98(6): 1278–1284 Seow W Y, Hauser C A (2016). Freeze–dried agarose gels: A cheap, simple and recyclable adsorbent for the purification of methylene blue from industrial wastewater. Journal of Environmental Chemical Engineering, 4(2): 1714–1721 Sharma M, Chaudhary J P, Mondal D, Meena R, Prasad K (2015). A green and sustainable approach to utilize bio-ionic liquids for the selective precipitation of high purity agarose from an agarophyte extract. Green Chemistry, 17(5): 2867–2873 Sousa A M, Alves V D, Morais S, Delerue-Matos C, Gonçalves M P (2010). Agar extraction from integrated multitrophic aquacultured Gracilaria vermiculophylla: Evaluation of a microwave-assisted process using response surface methodology. Bioresource Technology, 101(9): 3258–3267 Trivedi T J, Kumar A (2014). Efficient extraction of agarose from red algae using ionic liquids. Green and Sustainable Chemistry, 4(4): 190–201 Wang L, Shen Z, Mu H, Lin Y, Zhang J, Jiang X (2017). Impact of alkali pretreatment on yield, physico-chemical and gelling properties of high quality agar from Gracilaria tenuistipitata. Food Hydrocolloids, 70: 356–362 Wang T P, Chang L L, Chang S N, Wang E C, Hwang L C, Chen Y H, Wang Y M (2012). Successful preparation and characterization of biotechnological grade agarose from indigenous Gelidium amansii of Taiwan. Process Biochemistry, 47(3): 550–554 Żyła G, Stompor M, Kopanska M, Stagraczynski R, Fal J, Traciak J, Trybus M, Wolski S, Zawlik I, Cebulski J, Konefal-Janocha M, Cholewa M (2017). The influence of sonication and silver nanoparticles doped on viscoelastic structure of Agarose Gel. Acta Physica Polonica A, 132(1): 152–154