Solid Phase Synthesis of Dual Labeled Peptides: Development of Cell Permeable Calpain Specific Substrates

Springer Science and Business Media LLC - Tập 13 - Trang 83-91 - 2006
Brian M. Polster1, Rafael Arze2, Matthew H. Lyttle2, David G. Nicholls1, Derek Hudson2
1Buck Institute for Age Research, Novato, USA
2Biosearch Technologies Inc, Novato, USA

Tóm tắt

A step-by-step evaluation of dual-labeled FRET substrates for the protease calpain is reported. The study led to cell permeable selections, with optimized specificity and effectiveness for the target enzyme, and improved stability to non-specific degrading enzymes.

Tài liệu tham khảo

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