Smart and sustainable: Exploring the future of PHAs biopolymers for 3D printing in tissue engineering

Sustainable Materials and Technologies - Tập 38 - Trang e00750 - 2023
Joanna Żur-Pińska1, Magdalena Z. Gładysz2,3, Didi Ubels2, Jeroen Siebring4, Małgorzata K. Włodarczyk-Biegun1,2
1Biotechnology Centre, Silesian University of Technology, Krzywoustego 8, 44-100, Gliwice, Poland
2Polymer Science, Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG, the Netherlands
3Pharmaceutical Analysis, Groningen Research Institute of Pharmacy, University of Groningen, P.O. Box 196, XB20, 9700 AD Groningen, the Netherlands
4Bioconversion and Fermentation Technology, Research Centre Biobased Economy, Hanze University of Applied Sciences, Zernikeplein 11, 9747 AS Groningen, the Netherlands

Tài liệu tham khảo

Anjum, 2016, Microbial production of polyhydroxyalkanoates (PHAs) and its copolymers: a review of recent advancements, Int. J. Biol. Macromol., 89, 161, 10.1016/j.ijbiomac.2016.04.069 Whenish, 2022, A framework for the sustainability implications of 3D bioprinting through nature-inspired materials and structures, Bio-Des. Manuf., 5, 412, 10.1007/s42242-021-00168-x Mohanty, 2022, Sustainable polymers, Nat. Rev. Dis. Primers., 2, 46, 10.1038/s43586-022-00124-8 Chen, 2015, White biotechnology for biopolymers: Hydroxyalkanoates and polyhydroxyalkanoates: Production and applications Roohi, 2018, Kuddus, PHB (poly-β-hydroxybutyrate) and its enzymatic degradation, Polym. Adv. Technol., 29, 30, 10.1002/pat.4126 Sanhueza, 2019, Polyhydroxyalkanoates as biomaterial for electrospun scaffolds, Int. J. Biol. Macromol., 124, 102, 10.1016/j.ijbiomac.2018.11.068 Pramanik, 2023, A tool for biomedical application: synthesis and modification of polyhydroxyalkanoates, Sustain. Chem. Pharm., 32 Ivorra-Martinez, 2023, The effects of processing parameters on mechanical properties of 3D-printed polyhydroxyalkanoates parts, Virt. Phys. Prototyp., 18 Sabarinathan, 2018, Production of polyhydroxybutyrate (PHB) from pseudomonas plecoglossicida and its application towards cancer detection, Inform. Med. Unlocked, 11, 61, 10.1016/j.imu.2018.04.009 Kaniuk, 2021, Development and advantages of biodegradable PHA polymers based on electrospun PHBV fibers for tissue engineering and other biomedical applications, ACS Biomater Sci. Eng., 7, 5339, 10.1021/acsbiomaterials.1c00757 Rett, 2021, Sustainable materials for fused deposition modeling 3D printing applications, Adv. Eng. Mater., 23, 2001472, 10.1002/adem.202001472 Abbel, 2022, Crystallization behavior and sensing properties of bio-based conductive composite materials, Adv. Eng. Mater., 25, 2200959, 10.1002/adem.202200959 Kovalcik, 2021, Recent advances in 3D printing of polyhydroxyalkanoates: a review, Eur. J. Dermatol., 5, 48 Mehrpouya, 2021, Additive manufacturing of polyhydroxyalkanoates (PHAs) biopolymers: materials, printing techniques, and applications, Mater. Sci. Eng. C, 127, 10.1016/j.msec.2021.112216 Steinbüchel, 2003, Metabolic engineering and pathway construction for biotechnological production of relevant polyhydroxyalkanoates in microorganisms, Biochem. Eng. J., 16, 81, 10.1016/S1369-703X(03)00036-6 Obruca, 2020, Novel unexpected functions of PHA granules, Appl. Microbiol. Biotechnol., 104, 4795, 10.1007/s00253-020-10568-1 Madison, 1999, Metabolic engineering of poly (3-hydroxyalkanoates): from DNA to plastic, Microbiol. Mol. Biol. Rev., 63, 21, 10.1128/MMBR.63.1.21-53.1999 Guo, 2022, Polyhydroxyalkanoates in tissue repair and regeneration, Eng. Regenerat., 3, 24 Kovalcik, 2019, Polyhydroxyalkanoates: their importance and future, BioRes, 14, 2468 Sehgal, 2020, Polyhydroxyalkanoate and its efficient production: an eco-friendly approach towards development, Biotech, 10, 549 Koller, 2018, Chemical and biochemical engineering approaches in manufacturing polyhydroxyalkanoate (PHA) biopolyesters of tailored structure with focus on the diversity of building blocks, Chem. Biochem. Eng. Q., 32, 413, 10.15255/CABEQ.2018.1385 Fukada, 1986, Piezoelectric properties of poly-β-hydroxybutyrate and copolymers of β-hydroxybutyrate and β-hydroxyvalerate, Int. J. Biol. Macromol., 8, 361, 10.1016/0141-8130(86)90056-5 Garcia-Garcia, 2022, Innovative solutions and challenges to increase the use of poly (3-hydroxybutyrate) in food packaging and disposables, Eur. Polym. J., 178, 10.1016/j.eurpolymj.2022.111505 Akdogan, 2021, Enhanced production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) biopolymer by recombinant bacillus megaterium in fed-batch bioreactors, Bioprocess Biosyst. Eng., 44, 403, 10.1007/s00449-020-02452-z Avella, 2000, Properties of blends and composites based on poly(3-hydroxy)butyrate (PHB) and poly(3-hydroxybutyrate-hydroxyvalerate) (PHBV) copolymers, J. Mater. Sci., 35, 523, 10.1023/A:1004740522751 Kenar, 2010, Design of a 3D aligned myocardial tissue construct from biodegradable polyesters, J. Mater. Sci. Mater. Med., 21, 989, 10.1007/s10856-009-3917-8 Prabhakaran, 2013, Electrospun aligned PHBV/collagen nanofibers as substrates for nerve tissue engineering, Biotechnol. Bioeng., 110, 2775, 10.1002/bit.24937 Martin, 2003, Medical applications of poly-4-hydroxybutyrate: a strong flexible absorbable biomaterial, Biochem. Eng. J., 16, 97, 10.1016/S1369-703X(03)00040-8 Mitra, 2021, Current advances towards 4-hydroxybutyrate containing polyhydroxyalkanoates production for biomedical applications, Molecules, 26, 7244, 10.3390/molecules26237244 Gregory, 2022, Polyhydroxyalkanoates and their advances for biomedical applications, Trends Mol. Med., 28, 331, 10.1016/j.molmed.2022.01.007 Williams, 2016, The history of GalaFLEX P4HB scaffold, Aesthet. Surg. J., 36, S33, 10.1093/asj/sjw141 Kim, 2005, Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by Ralstonia eutropha, Biochem. Eng. J., 23, 169, 10.1016/j.bej.2005.01.016 Fu, 2015, P34HB film promotes cell adhesion, in vitro proliferation, and in vivo cartilage repair, RSC Adv., 5, 21572, 10.1039/C5RA02016F Jian, 2022, Poly 3-hydroxybutyrate 4-hydroxybutyrate (P34HB) as a potential polymer for drug-eluting coatings on metal coronary stents, Polymers, 14, 994, 10.3390/polym14050994 Tanaka, 2021, Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from CO(2) by a recombinant Cupriavidus necator, Bioeng., 8, 179 Możejko-Ciesielska, 2019, Polyhydroxyalkanoates synthesized by Aeromonas species: trends and challenges, Polymers, 11, 1328, 10.3390/polym11081328 Doi, 1995, Microbial synthesis and characterization of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate), Macromolecules, 28, 4822, 10.1021/ma00118a007 Bian, 2009, Evaluation of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) conduits for peripheral nerve regeneration, Biomater., 30, 217, 10.1016/j.biomaterials.2008.09.036 Wang, 2004, Attachment, proliferation and differentiation of osteoblasts on random biopolyester poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) scaffolds, Biomater., 25, 669, 10.1016/S0142-9612(03)00561-1 Jiang, 2016, Carbon sources for polyhydroxyalkanoates and an integrated biorefinery, Int. J. Mol. Sci., 17, 1157, 10.3390/ijms17071157 Tan, 2021, Grand challenges for industrializing polyhydroxyalkanoates (PHAs), Trends Biotechnol., 39, 953, 10.1016/j.tibtech.2020.11.010 Wang, 1997, Poly(3-hydroxybutyrate) production with high productivity and high polymer content by a fed-batch culture of alcaligenes latus under nitrogen limitation, Appl. Environ. Microbiol., 63, 3703, 10.1128/aem.63.9.3703-3706.1997 Blunt, 2018, Bioreactor operating strategies for improved polyhydroxyalkanoate (PHA) productivity, Polymers, 10, 1197, 10.3390/polym10111197 Yu, 2019, Next-generation industrial biotechnology-transforming the current industrial biotechnology into competitive processes, Biotechnol. J., 14, 1800437, 10.1002/biot.201800437 Koller, 2022, A new wave of industrialization of PHA biopolyesters, Bioeng, 9, 74 Engelberg, 1991, Physico-mechanical properties of degradable polymers used in medical applications: a comparative study, Biomater., 12, 292, 10.1016/0142-9612(91)90037-B Shimamura, 1994, Physical properties and biodegradability of microbial poly(3-hydroxybutyrate-co-3-hydroxyhexanoate), Macromolecules, 27, 878, 10.1021/ma00081a041 Samui, 2019, Polyhydroxyalkanoates based copolymers, Int. J. Biol. Macromol., 140, 522, 10.1016/j.ijbiomac.2019.08.147 Syed, 2022, Polyhydroxyalkanoates (PHA)-based responsive polymers, Int. J. Polym. Mater. Polym., 71, 1283, 10.1080/00914037.2021.1962874 Bedian, 2017, Bio-based materials with novel characteristics for tissue engineering applications - a review, Int. J. Biol. Macromol., 98, 837, 10.1016/j.ijbiomac.2017.02.048 Zhao, 2021, Electrospinning nanofibers of microbial polyhydroxyalkanoates for applications in medical tissue engineering, J. Polym. Sci., 59, 1994, 10.1002/pol.20210418 Palmeiro-Sanchez, 2022, Polyhydroxyalkanoate bio-production and its rise as biomaterial of the future, J. Biotechnol., 348, 10, 10.1016/j.jbiotec.2022.03.001 Vigneswari, 2016, Simultaneous dual syringe electrospinning system using benign solvent to fabricate nanofibrous P(3HB-co-4HB)/collagen peptides construct as potential leave-on wound dressing, Mater. Sci. Eng. C, 66, 147, 10.1016/j.msec.2016.03.102 Yuan, 2009, Fabrication of PHBV/keratin composite nanofibrous mats for biomedical applications, Macromol. Res., 17, 850, 10.1007/BF03218625 Veleirinho, 2012, Nanofibrous poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/chitosan scaffolds for skin regeneration, Int. J. Biol. Macromol., 51, 343, 10.1016/j.ijbiomac.2012.05.023 Nagiah, 2013, Development and characterization of coaxially electrospun gelatin coated poly (3-hydroxybutyric acid) thin films as potential scaffolds for skin regeneration, Mater. Sci. Eng. C, 33, 4444, 10.1016/j.msec.2013.06.042 Naderi, 2020, Evaluation of the effects of keratin on physical, mechanical and biological properties of poly (3-hydroxybutyrate) electrospun scaffold: potential application in bone tissue engineering, Eur. Polym. J., 124, 10.1016/j.eurpolymj.2020.109502 Karbowniczek, 2021, Enhanced cells anchoring to electrospun hybrid scaffolds with PHBV and HA particles for bone tissue regeneration, Front. Bioeng. Biotechnol., 9, 10.3389/fbioe.2021.632029 Lei, 2015, Preparation and characterization of polyhydroxybutyrate-co-hydroxyvalerate/silk fibroin nanofibrous scaffolds for skin tissue engineering, Polym. Eng. Sci., 55, 907, 10.1002/pen.23958 Kouhi, 2018, Poly L lysine-modified PHBV based nanofibrous scaffolds for bone cell mineralization and osteogenic differentiation, Appl. Surf. Sci., 457, 616, 10.1016/j.apsusc.2018.06.239 Pascu, 2013, Electrospun composites of PHBV, silk fibroin and nano-hydroxyapatite for bone tissue engineering, Mater. Sci. Eng. C, 33, 4905, 10.1016/j.msec.2013.08.012 Wu, 2022, Nanocomposites of bio-base polyester containing natural hydroxyapatite and duck eggshell made by electrospinning: fabrication and characterization, J. Polym. Environ., 31, 519, 10.1007/s10924-022-02558-3 Kaniuk, 2020, Osteoblasts and fibroblasts attachment to poly (3-hydroxybutyric acid-co-3-hydrovaleric acid)(PHBV) film and electrospun scaffolds, Mater. Sci. Eng. C, 110, 10.1016/j.msec.2020.110668 Saska, 2018, Three-dimensional printing and in vitro evaluation of poly (3-hydroxybutyrate) scaffolds functionalized with osteogenic growth peptide for tissue engineering, Mater. Sci. Eng. C, 89, 265, 10.1016/j.msec.2018.04.016 Augustine, 2020, Cerium oxide nanoparticle incorporated electrospun poly(3-hydroxybutyrate-co-3-hydroxyvalerate) membranes for diabetic wound healing applications, ACS Biomater Sci. Eng., 6, 58, 10.1021/acsbiomaterials.8b01352 Ji, 2008, Interactions between a poly (3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) terpolyester and human keratinocytes, Biomaterials, 29, 3807, 10.1016/j.biomaterials.2008.06.008 Zhu, 2007, Proteins combination on PHBV microsphere scaffold to regulate Hep3B cells activity and functionality: a model of liver tissue engineering system, J. Biomed. Mater. Res. A, 83, 606, 10.1002/jbm.a.31257 Zhao, 2014, Three dimensionally printed mesoporous bioactive glass and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) composite scaffolds for bone regeneration, J. Mater. Chem. B, 2, 6106, 10.1039/C4TB00838C Grande, 2017, Design of functionalized biodegradable PHA-based electrospun scaffolds meant for tissue engineering applications, New Biotechnol., 37, 129, 10.1016/j.nbt.2016.05.006 Chen, 2018, Microbial polyhydroxyalkanoates as medical implant biomaterials, Artif. Cells Nanomed, Biotechnol., 46, 1 Lim, 2017, Emerging bone tissue engineering via polyhydroxyalkanoate (PHA)-based scaffolds, Mater. Sci. Eng. C, 79, 917, 10.1016/j.msec.2017.05.132 Qu, 2012, In vitro study on hemocompatibility and cytocompatibility of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate), J. Biomater. Sci. Polym. Ed., 17, 1107, 10.1163/156856206778530704 Elyaderani, 2022, Multifunctional scaffolds based on emulsion and coaxial electrospinning incorporation of hydroxyapatite for bone tissue regeneration, Int. J. Mol. Sci., 23, 15016, 10.3390/ijms232315016 Chen, 2009, A microbial polyhydroxyalkanoates (PHA) based bio-and materials industry, Chem. Soc. Rev., 38, 2434, 10.1039/b812677c Jendrossek, 1996, Biodegradation of polyhydroxyalkanoic acids, Appl. Microbiol. Biotechnol., 46, 451, 10.1007/s002530050844 Hiraishi, 2009, Enzyme-catalyzed synthesis and degradation of biopolymers, Mini-Rev. Org, 6, 44, 10.2174/157019309787316139 Shishatskaya, 2005, Degradation of P (3HB) and P (3HB-co-3HV) in biological media, J. Biomater. Sci. Polym. Ed., 16, 643, 10.1163/1568562053783678 Choi, 2004, Effect of organosoluble salts on the nanofibrous structure of electrospun poly (3-hydroxybutyrate-co-3-hydroxyvalerate), Int. J. Biol. Macromol., 34, 249, 10.1016/j.ijbiomac.2004.06.001 Chuah, 2013, Biosynthesis and characterization of polyhydroxyalkanoate containing 5-hydroxyvalerate units: effects of 5HV units on biodegradability, cytotoxicity, mechanical and thermal properties, Polym. Degrad. Stab., 98, 331, 10.1016/j.polymdegradstab.2012.09.008 Wei, 2017, Stimuli-responsive polymers and their applications, Polym. Chem., 8, 127, 10.1039/C6PY01585A Li, 2020, Bacteria-triggered release of a potent biocide from core-shell polyhydroxyalkanoate (PHA)-based nanofibers for wound dressing applications, J. Biomater. Sci. Polym. Ed., 31, 394, 10.1080/09205063.2019.1693882 Wang, 2019, Development of polyhydroxyalkanoate-based polyurethane with water-thermal response shape-memory behavior as new 3D elastomers scaffolds, Polymers, 11, 1030, 10.3390/polym11061030 Le Duigou, 2016, 3D printing of wood fibre biocomposites: from mechanical to actuation functionality, Mater. Des., 96, 106, 10.1016/j.matdes.2016.02.018 Cao, 2018, PLLA-PHB fiber membranes obtained by solvent-free electrospinning for short-time drug delivery, Drug Deliv Transl. Res., 8, 291, 10.1007/s13346-017-0463-7 Maji, 2021, Electrospun scaffold for bone regeneration, Int. J. Polym. Mater. Polym., 71, 842, 10.1080/00914037.2021.1915784 Giubilini, 2023, Novel 3D printable bio-based and biodegradable poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) microspheres for selective laser sintering applications, Mater. Today Sustain., 22 Kosorn, 2017, PCL/PHBV blended three dimensional scaffolds fabricated by fused deposition modeling and responses of chondrocytes to the scaffolds, J. Biomed. Mater. Res. B, 105, 1141, 10.1002/jbm.b.33658 Ausejo, 2018, A comparative study of three-dimensional printing directions: the degradation and toxicological profile of a PLA/PHA blend, Polym. Degrad. Stab., 152, 191, 10.1016/j.polymdegradstab.2018.04.024 Martins, 2021, A versatile filler in polyhydroxyalcanoates filaments for FDM: a diverse panorama for pullulan application, Mater. Today Commun, 28, 102690, 10.1016/j.mtcomm.2021.102690 Gonzalez, 2018, Three-dimensional printing of PLA and PLA/PHA dumbbell-shaped specimens of crisscross and transverse patterns as promising materials in emerging application areas: prediction study, Polym. Degrad. Stab., 156, 100, 10.1016/j.polymdegradstab.2018.08.008 Rydz, 2020, Three-dimensional printed PLA and PLA/PHA dumbbell-shaped specimens: material defects and their impact on degradation behavior, Materials, 13, 10.3390/ma13082005 Cecen, 2023, FDM-based 3D printing of PLA/PHA composite polymers, Chem. Pap., 10.1007/s11696-023-02786-4 Kontarova, 2020, Printability, mechanical and thermal properties of poly(3-hydroxybutyrate)-poly(lactic acid)-plasticizer blends for three-dimensional (3D) printing, Materials, 13, 4736, 10.3390/ma13214736 Laoutid, 2022, Impact-resistant poly(3-hydroxybutyrate)/poly(epsilon-caprolactone)-based materials, through reactive melt processing, for compression-molding and 3D-printing applications, Materials, 15, 8233, 10.3390/ma15228233 Wu, 2017, Characterisation, biodegradability and application of palm fibre-reinforced polyhydroxyalkanoate composites, Polym. Degrad. Stab., 140, 55, 10.1016/j.polymdegradstab.2017.04.016 Chiulan, 2017, Recent advances in 3D printing of aliphatic polyesters, Bioengineering, 5, 2, 10.3390/bioengineering5010002 Pereira, 2012, 3D printing of poly(3-hydroxybutyrate) porous structures using selective laser sintering, Macromol. Symp., 319, 64, 10.1002/masy.201100237 Pereira, 2012, Effect of process parameters on the properties of selective laser sintered poly(3-hydroxybutyrate) scaffolds for bone tissue engineering, Virtual Phys. Prototyp., 7, 275, 10.1080/17452759.2012.738551 Duan, 2010, Customized ca-P/PHBV nanocomposite scaffolds for bone tissue engineering: design, fabrication, surface modification and sustained release of growth factor, J. R. Soc. Interface, 7, S615, 10.1098/rsif.2010.0127.focus Duan, 2010, Encapsulation and release of biomolecules from ca–P/PHBV nanocomposite microspheres and three-dimensional scaffolds fabricated by selective laser sintering, Polym. Degrad. Stab., 95, 1655, 10.1016/j.polymdegradstab.2010.05.022 Duan, 2011, Optimized fabrication of ca-P/PHBV nanocomposite scaffolds via selective laser sintering for bone tissue engineering, Biofabrication, 3, 10.1088/1758-5082/3/1/015001 Dalton, 2017, Melt electrowriting with additive manufacturing principles, Curr. Opin. Biomed. Eng., 2, 49, 10.1016/j.cobme.2017.05.007 Xue, 2019, Electrospinning and electrospun nanofibers: methods, materials, and applications, Chem. Rev., 119, 5298, 10.1021/acs.chemrev.8b00593 Tong, 2010, Electrospinning of fibrous polymer scaffolds using positive voltage or negative voltage: a comparative study, Biomed. Mater., 5, 10.1088/1748-6041/5/5/054110 Yoon, 2008, Superhydrophobicity of PHBV fibrous surface with bead-on-string structure, J. Colloid Interface Sci., 320, 91, 10.1016/j.jcis.2008.01.029 Zhu, 2006, Superhydrophobic surface directly created by electrospinning based on hydrophilic material, J. Mater. Sci., 41, 3793, 10.1007/s10853-005-5910-z Galego, 2000, Characterization and application of poly (β-hydroxyalkanoates) family as composite biomaterials, Polym. Test., 19, 485, 10.1016/S0142-9418(99)00011-2 Rai, 2011, Medium chain length polyhydroxyalkanoates, promising new biomedical materials for the future, Mater. Sci. Eng. R. Rep., 72, 29, 10.1016/j.mser.2010.11.002 Ang, 2020, Electrospun poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)/silk fibroin film is a promising scaffold for bone tissue engineering, Int. J. Biol. Macromol., 145, 173, 10.1016/j.ijbiomac.2019.12.149 Unalan, 2016, Biocompatibility of plasma-treated poly(3-hydroxybutyrate-co-3-hydroxyvalerate) nanofiber mats modified by silk fibroin for bone tissue regeneration, Mater. Sci. Eng. C, 68, 842, 10.1016/j.msec.2016.07.054 Biazar, 2013, A nanofibrous PHBV tube with Schwann cell as artificial nerve graft contributing to rat sciatic nerve regeneration across a 30-mm defect bridge, Cell Commun. Adhes., 20, 41, 10.3109/15419061.2013.774378 Castellano, 2014, A comparison of electrospun polymers reveals poly(3-hydroxybutyrate) fiber as a superior scaffold for cardiac repair, Stem Cells Dev., 23, 1479, 10.1089/scd.2013.0578 Foroughi, 2017, Polyhydroxybutyrate/chitosan/bioglass nanocomposite as a novel electrospun scaffold: fabrication and characterization, J. Porous. Mater., 24, 1447, 10.1007/s10934-017-0385-2 Masaeli, 2013, Fabrication, characterization and cellular compatibility of poly(hydroxy alkanoate) composite nanofibrous scaffolds for nerve tissue engineering, PLoS One, 8, 10.1371/journal.pone.0057157 Liu, 2019, Synthesis of an electrospun PHA/RGO/au scaffold for peripheral nerve regeneration: an in vitro study, Appl. Nanosci., 10, 687, 10.1007/s13204-019-01130-1 Ramburrun, 2019, Design and characterisation of PHBV-magnesium oleate directional nanofibers for neurosupport, Biomed. Mater., 14, 10.1088/1748-605X/ab453c Antonova, 2021, Tissue-engineered carotid artery interposition grafts demonstrate high primary patency and promote vascular tissue regeneration in the ovine model, Polymers, 13, 2637, 10.3390/polym13162637 Ghadirian, 2023, Evaluation of the effects of halloysite nanotube on polyhydroxybutyrate - chitosan electrospun scaffolds for cartilage tissue engineering applications, Int. J. Biol. Macromol., 233, 10.1016/j.ijbiomac.2023.123651 Bhattacharjee, 2016, Fabrication and characterization of pluronic modified poly(hydroxybutyrate) fibers for potential wound dressing applications, Mater. Sci. Eng. C, 63, 266, 10.1016/j.msec.2016.02.074 Zhijiang, 2016, Poly(hydroxybutyrate)/cellulose acetate blend nanofiber scaffolds: preparation, characterization and cytocompatibility, Mater. Sci. Eng. C, 58, 757, 10.1016/j.msec.2015.09.048 Kim, 2007, Adhesion behavior of human bone marrow stromal cells on differentially wettable polymer surfaces, Tissue Eng., 13, 2095, 10.1089/ten.2006.0062 Lee, 1997, Interaction of cells on chargeable functional group gradient surfaces, Biomater., 18, 351, 10.1016/S0142-9612(96)00128-7 Karbasi, 2016, Preparation and characterization of poly (hydroxy butyrate)/chitosan blend scaffolds for tissue engineering applications, Adv. Biomed. Res., 5, 177, 10.4103/2277-9175.188490 Chen, 2021, A novel porous composite membrane of PHA/PVA via coupling of electrospinning and spin coating for antibacterial applications, Mater. Lett., 301, 10.1016/j.matlet.2021.130279 Wang, 2016, Differences in cytocompatibility between collagen, gelatin and keratin, Mater. Sci. Eng. C, 59, 30, 10.1016/j.msec.2015.09.093 Hu, 2003, Antibacterial and biodegradable properties of polyhydroxyalkanoates grafted with chitosan and chitooligosaccharides via ozone treatment, J. Appl. Polym. Sci., 88, 2797, 10.1002/app.12055 Kandhasamy, 2017, Synthesis and fabrication of collagen-coated ostholamide electrospun nanofiber scaffold for wound healing, ACS Appl. Mater. Interfaces, 9, 8556, 10.1021/acsami.6b16488 Kuntzler, 2018, Polyhydroxybutyrate and phenolic compounds microalgae electrospun nanofibers: a novel nanomaterial with antibacterial activity, Int. J. Biol. Macromol., 113, 1008, 10.1016/j.ijbiomac.2018.03.002 Douglass, 2021, S-nitrosoglutathione-based nitric oxide-releasing nanofibers exhibit dual antimicrobial and antithrombotic activity for biomedical applications, Macromol. Biosci., 21, 2000248, 10.1002/mabi.202000248 Mollaqasem, 2020, Incorporation of graphene oxide and calcium phosphate in the PCL/PHBV core-shell nanofibers as bone tissue scaffold, J. Appl. Polym. Sci., 138, 49797, 10.1002/app.49797 Amini, 2018, A novel bilayer drug-loaded wound dressing of PVDF and PHB/chitosan nanofibers applicable for post-surgical ulcers, Int. J. Polym. Mater. Polym., 68, 772, 10.1080/00914037.2018.1506982 Mutlu, 2018, Curcumin-loaded electrospun PHBV nanofibers as potential wound-dressing material, J. Drug Deliv. Sci. Technol., 43, 185, 10.1016/j.jddst.2017.09.017 Wu, 2018, Bio-based electrospun nanofiber of polyhydroxyalkanoate modified with black soldier fly’s pupa shell with antibacterial and cytocompatibility properties, ACS Appl. Mater. Interfaces, 10, 42127, 10.1021/acsami.8b16606 Hrynevich, 2018, Dimension-based design of melt electrowritten scaffolds, Small, 14, 1800232, 10.1002/smll.201800232 Xie, 2019, Structure-induced cell growth by 3D printing of heterogeneous scaffolds with ultrafine fibers, Mater. Des., 181, 10.1016/j.matdes.2019.108092 Robinson, 2019, The next frontier in melt electrospinning: taming the jet, Adv. Funct. Mater., 29, 1904664, 10.1002/adfm.201904664 Zielinski, 2023, 3D printing of bio-instructive materials: toward directing the cell, Bioact. Mater., 19, 292 Vyas, 2020, Three-dimensional printing and electrospinning dual-scale polycaprolactone scaffolds with low-density and oriented fibers to promote cell alignment, 3D print, Addit. Manuf., 7, 105 Yu, 2016, Fabrication and characterization of electrospinning/3D printing bone tissue engineering scaffold, RSC Adv., 6, 110557, 10.1039/C6RA17718B Huang, 2020, Engineered dual-scale poly (ε-caprolactone) scaffolds using 3D printing and rotational electrospinning for bone tissue regeneration, Addit. Manuf., 36 Tarazona, 2020, Influence of depolymerases and lipases on the degradation of polyhydroxyalkanoates determined in langmuir degradation studies, Adv. Mater. Interfaces, 7, 2000872, 10.1002/admi.202000872 Volova, 2003, Results of biomedical investigations of PHB and PHB/PHV fibers, Biochem. Eng. J., 16, 125, 10.1016/S1369-703X(03)00038-X Matos, 2021, Sludge retention time impacts on polyhydroxyalkanoate productivity in uncoupled storage/growth processes, Sci. Total Environ., 799, 10.1016/j.scitotenv.2021.149363 Nayır, 2023, Extraction of polyhydroxyalkanoate from activated sludge using supercritical carbon dioxide process and biopolymer characterization, J. Biotechnol., 364, 50, 10.1016/j.jbiotec.2023.01.011 Qin, 2018, CRISPR/Cas9 editing genome of extremophile Halomonas spp, Metab. Eng., 47, 219, 10.1016/j.ymben.2018.03.018 Kovalcik, 2018, Influence of removal of microbial inhibitors on PHA production from spent coffee grounds employing Halomonas halophila, J. Environ. Chem. Eng., 6, 3495, 10.1016/j.jece.2018.05.028 Povolo, 2010, Polyhydroxyalkanoates production by engineered Cupriavidus necator from waste material containing lactose, Bioresour. Technol., 101, 7902, 10.1016/j.biortech.2010.05.029 Brandl, 1989, Ability of the phototrophic bacterium Rhodospirillum rubrum to produce various poly (β-hydroxyalkanoates): potential sources for biodegradable polyesters, Int. J. Biol. Macromol., 11, 49, 10.1016/0141-8130(89)90040-8 McQualter, 2015, The use of an acetoacetyl-CoA synthase in place of a beta-ketothiolase enhances poly-3-hydroxybutyrate production in sugarcane mesophyll cells, Plant Biotechnol. J., 13, 700, 10.1111/pbi.12298 Malik, 2015, Production of high levels of poly-3-hydroxybutyrate in plastids of Camelina sativa seeds, Plant Biotechnol. J., 13, 675, 10.1111/pbi.12290