Site-directed mutagenesis of histidine 93, aspartic acid 180, glutamic acid 205, histidine 290, and aspartic acid 291 at the active site and tryptophan 279 at the raw starch binding site in barley alpha-amylase 1.

Journal of Biological Chemistry - Tập 268 Số 30 - Trang 22480-22484 - 1993
Morten Søgaard1,2,3,4, Anders Kadziola1,2,3,4, R. Haser1,2,3,4, Birte Svensson1,2,3,4
1Chemical Laboratory IV, University of Copen-hagen, H.C. 0rsteds Institute, Universitetsparken 5, DK-2100 Copen-hagen 0, Denmark.
2From the Carlsberg Laboratory, Department of Chemistry, Gamle Carkberg Vej IO, DK-2500 Valby, Denmark and
3Program in Cellular Biochemistry & Biophysics, Rockefeller Research Laboratories, Memorial Sloan-Kettering Can-cer Center, 1275 York Ave., Box 251, New York, NY 10021.
4SLaboratoire de Cristallographie et Cristallisation des Macromolicules Biologiqws-Centre National de la Recherche Scientifique, Faculte de Medecine, Secteur Nord, Boulevard Pierre Dramrd, F-13326 Marseille Cedex 15, France

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