Single-cell metabolic imaging reveals a SLC2A3-dependent glycolytic burst in motile endothelial cells
Tóm tắt
Từ khóa
Tài liệu tham khảo
Kim, B., Li, J., Jang, C. & Arany, Z. Glutamine fuels proliferation but not migration of endothelial cells. EMBO J. 36, 2321–2333 (2017).
Wu, D. et al. HIF-1α is required for disturbed flow-induced metabolic reprogramming in human and porcine vascular endothelium. eLife 6, e25217 (2017).
Feng, S. et al. Mechanical activation of hypoxia-inducible factor 1α drives endothelial dysfunction at atheroprone sites. Arterioscler Thromb. Vasc. Biol. 37, 2087–2101 (2017).
Doddaballapur, A. et al. Laminar shear stress inhibits endothelial cell metabolism via KLF2-mediated repression of PFKFB3. Arterioscler Thromb. Vasc. Biol. 35, 137–145 (2015).
van Nieuw Amerongen, G. P., Koolwijk, P., Versteilen, A. & van Hinsbergh, V. W. Involvement of RhoA/Rho kinase signaling in VEGF-induced endothelial cell migration and angiogenesis in vitro. Arterioscler Thromb. Vasc. Biol. 23, 211–217 (2003).
Van Valen, D. A. et al. Deep learning automates the quantitative analysis of individual cells in live-cell imaging experiments. PLoS Comput. Biol. 12, e1005177 (2016).
Ronneberger, O., Fischer, P. & Brox, T. U-Net: convolutional networks for biomedical image segmentation. in Medical Image Computing and Computer-Assisted Intervention – MICCAI 2015. 234–241 (2015).
San Martin, A. et al. A genetically encoded FRET lactate sensor and its use to detect the Warburg effect in single cancer cells. PLoS ONE 8, e57712 (2013).
Csurka, G., Larlus, D. & Perronnin, F. What is a good evaluation measure for semantic segmentation? In Proc. British Machine Vision Conference (BMVA Press, 2013).
Sonveaux, P. et al. Targeting the lactate transporter MCT1 in endothelial cells inhibits lactate-induced HIF-1 activation and tumor angiogenesis. PLoS ONE 7, e33418 (2012).
San Martin, A. et al. Imaging mitochondrial flux in single cells with a FRET sensor for pyruvate. PLoS ONE 9, e85780 (2014).
Takanaga, H., Chaudhuri, B. & Frommer, W. B. GLUT1 and GLUT9 as major contributors to glucose influx in HepG2 cells identified by a high sensitivity intramolecular FRET glucose sensor. Biochim. Biophys. Acta 1778, 1091–1099 (2008).
Imamura, H. et al. Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators. Proc. Natl Acad. Sci. USA 106, 15651–15656 (2009).
Mayor, R. & Etienne-Manneville, S. The front and rear of collective cell migration. Nat. Rev. Mol. Cell Biol. 17, 97–109 (2016).
Diebold, L. P. et al. Mitochondrial complex III is necessary for endothelial cell proliferation during angiogenesis. Nat. Metab. 1, 158–171 (2019).
Mole, D. R. et al. 2-Oxoglutarate analogue inhibitors of hif prolyl hydroxylase. Bioorg. Medicinal Chem. Lett. 13, 2677–2680 (2003).
Fazal, F. et al. Essential role of cofilin-1 in regulating thrombin-induced RelA/p65 nuclear translocation and intercellular adhesion molecule 1 (ICAM-1) expression in endothelial cells. J. Biol. Chem. 284, 21047–21056 (2009).
van Nieuw Amerongen, G. P., Vermeer, M. A. & van Hinsbergh, V. W. Role of RhoA and Rho kinase in lysophosphatidic acid-induced endothelial barrier dysfunction. Arterioscler Thromb. Vasc. Biol. 20, E127–E133 (2000).
van Nieuw Amerongen, G. P., van Delft, S., Vermeer, M. A., Collard, J. G. & van Hinsbergh, V. W. Activation of RhoA by thrombin in endothelial hyperpermeability: role of Rho kinase and protein tyrosine kinases. Circ. Res. 87, 335–340 (2000).
Blanchoin, L., Boujemaa-Paterski, R., Sykes, C. & Plastino, J. Actin dynamics, architecture, and mechanics in cell motility. Physiol. Rev. 94, 235–263 (2014).
Goddard, L. M. & Iruela-Arispe, M. L. Cellular and molecular regulation of vascular permeability. Thromb. Haemost. 109, 407–415 (2013).
Mehta, D. & Malik, A. B. Signaling mechanisms regulating endothelial permeability. Physiol. Rev. 86, 279–367 (2006).
Vandenbroucke, E., Mehta, D., Minshall, R. & Malik, A. B. Regulation of endothelial junctional permeability. Ann. N. Y. Acad. Sci. 1123, 134–145 (2008).
Tang, M. et al. Brain microvasculature defects and Glut1 deficiency syndrome averted by early repletion of the glucose transporter-1 protein. Nat. Commun. 8, 14152 (2017).
Fessel, J. P. et al. Metabolomic analysis of bone morphogenetic protein receptor type 2 mutations in human pulmonary endothelium reveals widespread metabolic reprogramming. Pulm. Circ. 2, 201–213 (2012).
Sasmal, D. K. et al. TCR–pMHC bond conformation controls TCR ligand discrimination. Cell Mol. Immunol. 17, 203–217 (2020).
Sasmal, D. K., Pulido, L. E., Kasal, S. & Huang, J. Single-molecule fluorescence resonance energy transfer in molecular biology. Nanoscale 8, 19928–19944 (2016).
Fraccaroli, A. et al. Endothelial α-parvin controls integrity of developing vasculature and is required for maintenance of cell-cell junctions. Circ. Res. 117, 29–40 (2015).
Khan, S. et al. EndoDB: a database of endothelial cell transcriptomics data. Nucleic Acids Res. 47, D736–D744 (2019).
Wu, C. et al. Mechanosensitive PPAP2B regulates endothelial responses to atherorelevant hemodynamic forces. Circ. Res. 117, e41–e53 (2015).
Yao, L., Xue, X., Yu, P., Ni, Y. & Chen, F. Evans blue dye: a revisit of its applications in biomedicine. Contrast Media Mol. Imaging 2018, 7628037 (2018).
Pertz, O., Hodgson, L., Klemke, R. L. & Hahn, K. M. Spatiotemporal dynamics of RhoA activity in migrating cells. Nature 440, 1069–1072 (2006).
Wilhelm, K. et al. FOXO1 couples metabolic activity and growth state in the vascular endothelium. Nature 529, 216–220 (2016).
Karmaus, P. W. F. et al. Metabolic heterogeneity underlies reciprocal fates of TH17 cell stemness and plasticity. Nature 565, 101–105 (2019).
Bantug, G. R., Galluzzi, L., Kroemer, G. & Hess, C. The spectrum of T cell metabolism in health and disease. Nat. Rev. Immunol. 18, 19–34 (2018).
Hensley, ChristopherT. et al. Metabolic heterogeneity in human lung tumors. Cell 164, 681–694 (2016).
van Heerden, J. H. et al. Lost in transition: start-up of glycolysis yields subpopulations of nongrowing cells. Science 343, 1245114 (2014).
Huang, J. et al. A single peptide-major histocompatibility complex ligand triggers digital cytokine secretion in CD4+ T cells. Immunity 39, 846–857 (2013).
Masters, C. Interactions between glycolytic enzymes and components of the cytomatrix. J. Cell Biol. 99, 222s–225s (1984).
Huang, Y. et al. Normal glucose uptake in the brain and heart requires an endothelial cell-specific HIF-1α-dependent function. Proc. Natl Acad. Sci. USA 109, 17478–17483 (2012).
Kuo, A., Lee, M. Y. & Sessa, W. C. Lipid droplet biogenesis and function in the endothelium. Circ. Res. 120, 1289–1297 (2017).
Sorbara, L. R. et al. Thrombin-induced translocation of GLUT3 glucose transporters in human platelets. Biochem. J. 328, 511–516 (1997).
Huang, R. T. et al. Experimental lung injury reduces Kruppel-like factor 2 to increase endothelial permeability via regulation of RAPGEF3–Rac1 signaling. Am. J. Respir. Crit. Care Med 195, 639–651 (2017).
Zhang, D. et al. Metabolic regulation of gene expression by histone lactylation. Nature 574, 575–580 (2019).
Li, L. et al. Artificial virus delivers CRISPR–Cas9 system for genome editing of cells in mice. ACS Nano 11, 95–111 (2017).
Barbee, K. A., Davies, P. F. & Lal, R. Shear stress-induced reorganization of the surface topography of living endothelial cells imaged by atomic force microscopy. Circ. Res. 74, 163–171 (1994).