Simultaneous evaluation of cell viability by neutral red, MTT and crystal violet staining assays of the same cells

Toxicology in Vitro - Tập 12 Số 3 - Trang 251-258 - 1998
Kenichi Chiba1, Keisuke Kawakami1, Kaoru Tohyama1
1Department of Toxicology, Safety Research Center, Yakult Institute for Microbiological Research, 1796 Kunitachi-shi yaho, Tokyo, 186-8650, Japan

Tóm tắt

Từ khóa


Tài liệu tham khảo

Allen, 1991, Toxic effects of non-steroidal anti-inflammatory drugs in a human intestinal epithelial cell line (HCT-8) as assessed by the MTT and neutral red assays, Toxicology in Vitro, 5, 183, 10.1016/0887-2333(91)90016-7

Arranz, 1990, Prior use of the neutral red assay and reduction of total protein determination in 96-well plate assays, Toxicology in Vitro, 4, 211, 10.1016/0887-2333(90)90024-N

Babich, 1987, Structure-activity relationship (SAR) model established in vitro with the neutral red cytotoxicity assay, Toxicology in Vitro, 1, 3, 10.1016/0887-2333(87)90031-2

Borenfreund, 1985, Toxicity determined in vitro by morphological alterations and neutral red absorption, Toxicology Letters, 24, 119, 10.1016/0378-4274(85)90046-3

Borenfreund, 1988, Comparisons of two in vitro cytotoxicity assay-the neutral red (NR) and tetrazolium MTT tests, Toxicology in Vitro, 2, 1, 10.1016/0887-2333(88)90030-6

Cornelis, 1992, Prediction of eye irritancy potential of surfactants by cytotoxicity tests in vitro on cultures of human skin fibroblasts and keratinocytes, Toxicology in Vitro, 6, 119, 10.1016/0887-2333(92)90004-B

Denizot, 1986, Rapid colorimetric assay for cell growth and survival: Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability, Journal of Immunological Methods, 89, 271, 10.1016/0022-1759(86)90368-6

Forsby, 1991, The cytotoxicity of 22 sesquiterpenoid unsaturated dialdehydes, as determined by the neutral red absorption assay and protein determination, Toxicology in Vitro, 5, 9, 10.1016/0887-2333(91)90043-D

Hoh, 1987, Multilayered keratinocyte culture used for in vitro toxicology, Molecular Toxicology, 1, 537

Itagaki, 1991, An in vitro alternatine to the Darize eye irritation test: evaluation of the crystal violet staining method, Toxicology in Vitro, 5, 139, 10.1016/0887-2333(91)90034-B

Jabbar, 1989, The MTT assay underestimates the growth inhibitory effects of interferons, British Journal of Cancer, 60, 523, 10.1038/bjc.1989.306

Knox, 1986, The Frame multicentre project on in vitro cytotoxicology, Food and Chemical Toxicology, 24, 457, 10.1016/0278-6915(86)90092-X

Korting, 1994, MTT-assay and neutral red release (NRR)-assay: relative role in the prediction of the irritancy potential of surfactants, Life Sciences, 55, 533, 10.1016/0024-3205(94)00746-2

Mosmann, 1983, Rapid colorimetric assay for cellular growth and survivals: application to proliferation and cytotoxicity assays, Journal of Immunological Methods, 65, 55, 10.1016/0022-1759(83)90303-4

Nordin, 1991, Effect of exposure period of acetylsalicylic acid, paracetamol and isopropanol on L929 cytotoxicity, Toxicology in Vitro, 5, 449, 10.1016/0887-2333(91)90070-T

Ohkuma, 1981, Cytoplasmic vacuolation of mouse peritoneal macrophages and the uptake into lysosomes of weakly basic substances, Journal of Cell Biology, 90, 656, 10.1083/jcb.90.3.656

Olivier, 1995, Effect of high polyol concentrations on the neutral red absorption assay and tetrazolium-MTT test of rat hepatocytes in primary culture, Toxicology in Vitro, 9, 133, 10.1016/0887-2333(94)00209-D

Riddell, 1986, An evaluation of three in vitro cytotoxicity assays, Food and Chemical Toxicology, 24, 469, 10.1016/0278-6915(86)90095-5

Saotome, 1989, Cytotoxicity test with simplified crystal violet staining method using microtitre plates and its application to injection drugs, Toxicology in Vitro, 3, 317, 10.1016/0887-2333(89)90039-8

Triglia D., Braa S. S., Donnelly T., Kiddy I. and Naughton G. K. (1991a) A 3-dimensional human dermal model substrate for in vitro toxicological studies. In Alternative Methods in Toxicology. Edited by A. M. Goldberg and M. L. Principe. Vol. 8. pp. 351–362. Mary Ann Liebert, New York

Triglia, 1991, Cytotoxicity testing using neutral red and mtt assays on a three-dimensional human skin substrate, Toxicology in Vitro, 5, 573, 10.1016/0887-2333(91)90096-V

van de Sandt, 1993, Cutaneous toxicity testing in organ culture: neutral red uptake and reduction of tetrazolium salt (MTT), Toxicology in Vitro, 7, 81, 10.1016/0887-2333(93)90115-L

Vian, 1995, Comparison of three in vitro cytotoxicity assays for estimating surfactant ocular irritation, Toxicology in Vitro, 9, 185, 10.1016/0887-2333(94)00200-E

Vistica, 1991, Tetrazolium-based assays for cellular viability: a critical examination of selected parameters affecting formazan production, Cancer Research, 51, 2515