Simplified spectrophotometric assay for microsomal 3-hydroxy-3-methylglutaryl CoA reductase by measurement of coenzyme A

Regen, 1966, The measurement of β-hydroxy-β-methyl-glutaryl-CoA reductase in rat liver; effects of fasting and refeeding, Biochem. Z, 346, 78 Siperstein, 1966, Feedback control of mevalonate synthesis by dietary cholesterol, J. Biol. Chem, 241, 602, 10.1016/S0021-9258(18)96879-1 White, 1970, Biosynthesis of 3. -hydroxy-3-methylglutarate and mevalonate by rat liver homogenates in vitro, Biochemistry, 9, 2713, 10.1021/bi00815a020 Linn, 1967, The demonstration and solubilization of β-hydroxy-β-methylglutaryl coenzyme A reductase from rat liver microsomes, J. Biol. Chem, 242, 984, 10.1016/S0021-9258(18)96220-4 Retey, 1970, A probable intermediate in the enzymic reduction of 3-hy-droxy-3-methylglutaryl coenzyme A, Eur. J. Biochem, 15, 72, 10.1111/j.1432-1033.1970.tb00977.x Goldfarb, 1971, Improved assay of 3-hydroxy-3-methylglutaryl coenzyme A reductase, J. Lipid Res, 12, 512, 10.1016/S0022-2275(20)39504-3 Shapiro, 1971, Regulation of hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase and cholesterol synthesis, J. Biol. Chem, 246, 3210, 10.1016/S0021-9258(18)62216-1 Shefer, 1972, HMG CoA reductase of intestinal mucosa and liver of the rat, J. Lipid Res, 13, 402, 10.1016/S0022-2275(20)39404-9 Siperstein, 1966, A gas-liquid chromatographic procedure for the measurement of mevalonic acid synthesis, J. Biol. Chem, 241, 597601, 10.1016/S0021-9258(18)96878-X Ellman, 1959, Tissue sulfhydryl groups, Arch. Biochem. Biophys, 82, 70, 10.1016/0003-9861(59)90090-6 Zahler, 1968, A specific and sensitive assay for disulfides, J. Biol. Chem, 243, 716, 10.1016/S0021-9258(19)81724-6 Lowry, 1951, Protein measurement with the Folin phenol reagent, J. Biol. Chem, 193, 265, 10.1016/S0021-9258(19)52451-6 Shapiro, 1969, Thin-layer chromatographic assay for HMG-CoA reductase and mevalonic acid, Anal. Biochem, 31, 383, 10.1016/0003-2697(69)90279-6 Louw, 1969, Improved synthesis of 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA), J. Lipid Res, 10, 683, 10.1016/S0022-2275(20)43031-7 Berndt, 1971, Eine vereinfachte Me-thode zur Bestimmung der Hydroxymethylglutaryl-CoA-Re-duktase, Hoppe-Seyler's Z. Physiol. Chem, 352, 345, 10.1515/bchm2.1971.352.1.345 Kawachi, 1970, Solubilization and purification of β-hydroxy-β-methylglutaryl coenzyme A reductase from rat liver, Biochemistry, 9, 1700, 10.1021/bi00810a008