Silencing ZIC2 abrogates tumorigenesis and anoikis resistance of non-small cell lung cancer cells by inhibiting Src/FAK signaling

Molecular Therapy - Oncolytics - Tập 22 - Trang 195-208 - 2021
Aibin Liu1,2, Huayan Xie1, Ronggang Li3, Liangliang Ren4, Baishuang Yang1, Longxia Dai1, Wenjie Lu4, Baoyi Liu4, Dong Ren4,5,6, Xin Zhang4,5,6, Qiong Chen1,2, Yanming Huang4, Ke Shi1,2
1Department of Geriatrics, Xiangya Hospital, Central South University, Changsha 410008, China
2National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, 410008, China
3Department of Pathology, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen University, Jiangmen 529030, China
4Clinical Experimental Center, Jiangmen Key Laboratory of Clinical Biobanks and Translational Research, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen University, Jiangmen 529030, China
5Dongguan Key Laboratory of Medical Bioactive Molecular Developmental and Translational Research, Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical University, Dongguan 523808, China
6Collaborative Innovation Center for Antitumor Active Substance Research and Development, Guangdong Medical University, Zhanjiang, 524023, China

Tài liệu tham khảo

Siegel, 2021, Cancer Statistics, 2021, CA Cancer J. Clin., 71, 7, 10.3322/caac.21654 Jacobsen, 2017, Timeliness of access to lung cancer diagnosis and treatment: A scoping literature review, Lung Cancer, 112, 156, 10.1016/j.lungcan.2017.08.011 Bhagwat, 2015, Targeting Transcription Factors in Cancer, Trends Cancer, 1, 53, 10.1016/j.trecan.2015.07.001 Houtmeyers, 2013, The ZIC gene family encodes multi-functional proteins essential for patterning and morphogenesis, Cell. Mol. Life Sci., 70, 3791, 10.1007/s00018-013-1285-5 Chervenak, 2013, Spatiotemporal expression of Zic genes during vertebrate inner ear development, Dev. Dyn., 242, 897, 10.1002/dvdy.23978 Inaguma, 2015, Addiction of pancreatic cancer cells to zinc-finger transcription factor ZIC2, Oncotarget, 6, 28257, 10.18632/oncotarget.4960 Aruga, 2010, Expression of ZIC family genes in meningiomas and other brain tumors, BMC Cancer, 10, 79, 10.1186/1471-2407-10-79 Yi, 2018, The expression status of ZIC2 as a prognostic marker for nasopharyngeal carcinoma, Int. J. Clin. Exp. Pathol., 11, 4446 Sakuma, 2010, Expression status of Zic family member 2 as a prognostic marker for oral squamous cell carcinoma, J. Cancer Res. Clin. Oncol., 136, 553, 10.1007/s00432-009-0689-y Zhu, 2015, ZIC2-dependent OCT4 activation drives self-renewal of human liver cancer stem cells, J. Clin. Invest., 125, 3795, 10.1172/JCI81979 Lu, 2017, Zic2 promotes tumor growth and metastasis via PAK4 in hepatocellular carcinoma, Cancer Lett., 402, 71, 10.1016/j.canlet.2017.05.018 Chan, 2011, Zic2 synergistically enhances Hedgehog signalling through nuclear retention of Gli1 in cervical cancer cells, J. Pathol., 225, 525, 10.1002/path.2901 Marchini, 2012, The zinc finger gene ZIC2 has features of an oncogene and its overexpression correlates strongly with the clinical course of epithelial ovarian cancer, Clin. Cancer Res., 18, 4313, 10.1158/1078-0432.CCR-12-0037 Liu, 2020, ZIC2 is downregulated and represses tumor growth via the regulation of STAT3 in breast cancer, Int. J. Cancer, 147, 505, 10.1002/ijc.32922 Pfister, 2007, Array-based profiling of reference-independent methylation status (aPRIMES) identifies frequent promoter methylation and consecutive downregulation of ZIC2 in pediatric medulloblastoma, Nucleic Acids Res., 35, e51, 10.1093/nar/gkm094 Jiang, 2020, A ten-gene signature-based risk assessment model predicts the prognosis of lung adenocarcinoma, BMC Cancer, 20, 782, 10.1186/s12885-020-07235-z Wei-Hua, 2020, ZIC2 promotes cancer stem cell traits via up-regulating OCT4 expression in lung adenocarcinoma cells, J. Cancer, 11, 6070, 10.7150/jca.44367 Güre, 2000, Serological identification of embryonic neural proteins as highly immunogenic tumor antigens in small cell lung cancer, Proc. Natl. Acad. Sci. USA, 97, 4198, 10.1073/pnas.97.8.4198 Collins, 2005, G1/S cell cycle arrest provides anoikis resistance through Erk-mediated Bim suppression, Mol. Cell. Biol., 25, 5282, 10.1128/MCB.25.12.5282-5291.2005 Horowitz, 2007, Combinatorial activation of FAK and AKT by transforming growth factor-beta1 confers an anoikis-resistant phenotype to myofibroblasts, Cell. Signal., 19, 761, 10.1016/j.cellsig.2006.10.001 Fofaria, 2014, Critical role of STAT3 in melanoma metastasis through anoikis resistance, Oncotarget, 5, 7051, 10.18632/oncotarget.2251 Ren, 2017, Oncogenic miR-210-3p promotes prostate cancer cell EMT and bone metastasis via NF-κB signaling pathway, Mol. Cancer, 16, 117, 10.1186/s12943-017-0688-6 Chaffer, 2011, A perspective on cancer cell metastasis, Science, 331, 1559, 10.1126/science.1203543 Leng, 2016, An integrin beta4-EGFR unit promotes hepatocellular carcinoma lung metastases by enhancing anchorage independence through activation of FAK-AKT pathway, Cancer Lett., 376, 188, 10.1016/j.canlet.2016.03.023 Tang, 2018, Downregulation of miR-133a-3p promotes prostate cancer bone metastasis via activating PI3K/AKT signaling, J. Exp. Clin. Cancer Res., 37, 160, 10.1186/s13046-018-0813-4 Mo, 2020, IQGAP1 promotes anoikis resistance and metastasis through Rac1-dependent ROS accumulation and activation of Src/FAK signalling in hepatocellular carcinoma, Br. J. Cancer, 123, 1154, 10.1038/s41416-020-0970-z Li, 2019, ZNF32 induces anoikis resistance through maintaining redox homeostasis and activating Src/FAK signaling in hepatocellular carcinoma, Cancer Lett., 442, 271, 10.1016/j.canlet.2018.09.033 Sood, 2010, Adrenergic modulation of focal adhesion kinase protects human ovarian cancer cells from anoikis, J. Clin. Invest., 120, 1515, 10.1172/JCI40802 Park, 2009, Cholesterol depletion induces anoikis-like apoptosis via FAK down-regulation and caveolae internalization, J. Pathol., 218, 337, 10.1002/path.2531 Paoli, 2013, Anoikis molecular pathways and its role in cancer progression, Biochim. Biophys. Acta, 1833, 3481, 10.1016/j.bbamcr.2013.06.026 Subramani, 2013, Tyrosine phosphorylation of CD13 regulates inflammatory cell-cell adhesion and monocyte trafficking, J. Immunol., 191, 3905, 10.4049/jimmunol.1301348 Chen, 2020, LINC00173.v1 promotes angiogenesis and progression of lung squamous cell carcinoma by sponging miR-511-5p to regulate VEGFA expression, Mol. Cancer, 19, 98, 10.1186/s12943-020-01217-2 Jin, 2018, The PLAG1-GDH1 Axis Promotes Anoikis Resistance and Tumor Metastasis through CamKK2-AMPK Signaling in LKB1-Deficient Lung Cancer, Mol. Cell, 69, 87, 10.1016/j.molcel.2017.11.025 Summy, 2003, Src family kinases in tumor progression and metastasis, Cancer Metastasis Rev., 22, 337, 10.1023/A:1023772912750 Cai, 2011, Differential transformation capacity of Src family kinases during the initiation of prostate cancer, Proc. Natl. Acad. Sci. USA, 108, 6579, 10.1073/pnas.1103904108 Lechertier, 2012, Focal adhesion kinase and tumour angiogenesis, J. Pathol., 226, 404, 10.1002/path.3018 Lambert, 2017, Emerging Biological Principles of Metastasis, Cell, 168, 670, 10.1016/j.cell.2016.11.037 Lyu, 2017, ZIC2 Is Essential for Maintenance of Latency and Is a Target of an Immediate Early Protein during Kaposi’s Sarcoma-Associated Herpesvirus Lytic Reactivation, J. Virol., 91, e00980-17, 10.1128/JVI.00980-17 2021, Retraction, J. Cell. Biochem., 122, 145, 10.1002/jcb.29851 Vural, 2005, Frequency of SOX Group B (SOX1, 2, 3) and ZIC2 antibodies in Turkish patients with small cell lung carcinoma and their correlation with clinical parameters, Cancer, 103, 2575, 10.1002/cncr.21088 Chen, 2020, Downregulation of the circadian rhythm regulator HLF promotes multiple-organ distant metastases in non-small cell lung cancer through PPAR/NF-κb signaling, Cancer Lett., 482, 56, 10.1016/j.canlet.2020.04.007 Ruan, 2019, Silencing LGR6 Attenuates Stemness and Chemoresistance via Inhibiting Wnt/β-Catenin Signaling in Ovarian Cancer, Mol. Ther. Oncolytics, 14, 94, 10.1016/j.omto.2019.04.002 Liu, 2021, Overexpression of the SARS-CoV-2 receptor ACE2 is induced by cigarette smoke in bronchial and alveolar epithelia, J. Pathol., 253, 17, 10.1002/path.5555