Silage fermentation and ruminal degradation of stylo prepared with lactic acid bacteria and cellulase

Animal Science Journal - Tập 88 Số 10 - Trang 1531-1537 - 2017
Mao Li1, Hanlin Zhou1, Xuejuan Zi2, Yimin Cai3
1Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan, China
2Institute of Tropical Agriculture and Forestry, Hainan University, Danzhou, Hainan, China
3National Agriculture and Food Research Organization, Institute of Livestock and Grassland Science, Tsukuba, Ibaraki, Japan

Tóm tắt

AbstractIn order to improve the silage fermentation of stylo (Stylosanthes guianensis) in tropical areas, stylo silages were prepared with commercial additives Lactobacillus plantarum Chikuso‐1 (CH1), L. rhamnasus Snow Lact L (SN), Acremonium cellulase (CE) and their combination as SN+CE or CH1 + CE, and the fermentation quality, chemical composition and ruminal degradation of these silages were studied. Stylo silages treated with lactic acid bacteria (LAB) or cellulase, the pH value and NH3‐N ⁄ total‐N were significantly (< 0.05) decreased while the ruminal degradability of dry matter (DM), crude protein (CP), neutral detergent fiber (aNDFom) and acid detergent fiber (ADFom) were significantly (< 0.05) increased compared to control. Compared to LAB or cellulase‐treated silages, the DM, CP contents and relative feed value (RFV), and the ruminal degradability in LAB plus cellulase‐treated silages were significantly (< 0.05) higher, but the aNDFom content was significantly (< 0.05) lower. CH1 + CE treatment was more effective in silage fermentation and ruminal degradation than SN+CE treatment. The results confirmed that LAB or LAB plus cellulase treatment could improve the fermentation quality, chemical composition and ruminal degradation of stylo silage. Moreover, the combined treatment with LAB and cellulase may have beneficial synergistic effects on ruminal degradation.

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Tài liệu tham khảo

Association of Official Analytical Chemists, 1990, Official Methods of Analysis

10.1016/j.smallrumres.2015.12.006

10.3168/jds.S0022-0302(99)75263-X

Cai Y, 2003, Application of lactic acid bacteria (Lactobacillus plantarum Chikuso‐1) for silage preparation of forage paddy rice, Journal of Japanese Society of Grassland Science, 49, 477

10.1111/j.1744-697X.2009.00168.x

10.1002/jsfa.7271

10.2527/2003.8141040x

10.1016/j.smallrumres.2015.03.011

10.3168/jds.2006-820

10.1016/j.anifeedsci.2007.05.039

10.1111/j.1744-697X.2012.00244.x

10.1111/grs.12060

Guodao L, 2005, Stylo in China: A tropical forage legume success story, Tropical Grasslands, 39, 215

Guodao L, 1997, Status of Stylosanthes development in other countries. II. Stylosanthes development and utilisation in China and south‐east Asia, Tropical Grasslands, 31, 460

10.1111/j.1365-2494.1991.tb02209.x

10.1111/j.1365-2494.1991.tb02208.x

10.5713/ajas.2004.799

10.1111/grs.12046

10.1016/j.anifeedsci.2014.06.016

Liu Q, 2012, Characteristics of isolated lactic acid bacteria and their effectiveness to improve stylo (Stylosanthes guianensis Sw.) silage quality at various temperatures, Animal Sciences Journal, 83, 128, 10.1111/j.1740-0929.2011.00937.x

Mcdonald P, 1991, The Biochemistry of Silage, 48

10.1016/j.anifeedsci.2009.06.001

10.1016/j.anifeedsci.2015.05.001

10.1007/s11250-014-0647-9

Napasirth V, 2015, Microbial population, chemical composition and silage fermentation of cassava residues, Animal Sciences Journal, 86, 279

10.1002/(SICI)1097-0010(199710)75:2<161::AID-JSFA854>3.0.CO;2-Q

10.1071/AN11307

10.2527/jas1978.473747x

SAS, 1996, User's Guide: Statistics, Version 6.12

10.1016/j.anifeedsci.2009.04.013

Sun Q, 2012, Fermentation quality and chemical composition of shrub silage treated with lactic acid bacteria inoculants and cellulase additives, Animal Sciences Journal, 83, 305, 10.1111/j.1740-0929.2011.00962.x

10.3168/jds.S0022-0302(91)78551-2

10.5713/ajas.2011.10170

10.1016/j.anifeedsci.2004.08.013