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Shouchella tritolerans sp. nov., một loài vi khuẩn kỵ khí tùy nghi được phân lập từ trầm tích biển
Tóm tắt
Một chủng vi khuẩn kiềm, muối và chịu nhiệt có chỉ định FJAT-45399T đã được phân lập từ trầm tích biển tại tỉnh Phúc Kiến, Trung Quốc. Chủng FJAT-45399T có dạng hình que, dương Gram và kị khí tùy nghi. Chủng này có sự tương đồng cao về trình tự gen 16S rRNA với các thành viên thuộc chi Shouchella. Hơn nữa, phân tích phát sinh chủng loài và phân tích gen cũng cho thấy chủng FJAT-45399T thuộc cụm với các thành viên của chi Shouchella. Sự phát triển của chủng FJAT-45399T được quan sát ở nhiệt độ 15–55 °C (tối ưu 45–50 °C), pH 7.0–13.0 (tối ưu 9.0) và 0–15% (w/v) NaCl (tối ưu 2%). Nó chứa MK-7 là menaquinone. Các lipit phân cực bao gồm diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) và một glycolipid không xác định (UGL) và lipid (UL). Các axit béo chính (> 10%) là C16:0 (22.8%), iso-C15:0 (21.3%), và anteiso-C15:0 (14.0%). Hàm lượng G + C của DNA điều tra được là 44.5%. Giá trị đồng nhất nucleotide trung bình (ANI) và giá trị lai DNA-DNA số (dDDH) giữa chủng FJAT-45399T và chủng loại liên quan gần nhất Shouchella clausii DSM 8716T (ANI 94.1% và dDDH 55.4%) đều thấp hơn mức ngưỡng phân loại loài. Dựa trên các kết quả trên, chủng FJAT-45399T được đề xuất là một loài mới của chi Shouchella với tên gọi Shouchella tritolerans sp. nov. Chủng mẫu là FJAT-45399T (= GDMCC 1.3098T = JCM 35613T).
Từ khóa
Tài liệu tham khảo
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