Serum soluble interleukin‐6 receptor in MRL/lpr mice is elevated with age and mediates the interleukin‐6 signal

European Journal of Immunology - Tập 23 Số 5 - Trang 1078-1082 - 1993
Hiroshi Suzuki1, Kiyoshi Yasukawa2, Takashi Saito2, Masashi Narazak3, Atsuhiko Hasegawa4, Tetsuya Taga3, T Kishimoto5
1Biotechnology Research Laboratory, Tosoh Corporation, Kanagawa, Japan
2Biotechnology Research Laboratory, Tosoh Corporation, Kanagawa.
3Institute for Molecular and Cellular Biology, Osaka University, Osaka
4Department of Veterinary Internal Medicine, Faculty of Agriculture, University of Tokyo, Tokyo
5Department of Medicine III, Osaka University Medical School, Osaka

Tóm tắt

AbstractThe characteristics of soluble interleukin‐6 receptor (sIL‐6R) in murine sera were examined. To investigate a relationship between serum sIL‐6R level and autoimmune diseases, quantitative analysis of serum sIL‐6R in MRL/lpr mice was performed by an enzyme‐linked immunosorbent assay. The serum sIL‐6R level in MRL/lpr mice of both sexes was below the detection limit (< 1.0 ng/ml) at 8 weeks of age, but it increased in accordance with age and reached 42 ± 9.3 ng/ml in female and 31 ± 13 ng/ml in male mice at 30 weeks of age. In MRL/+ mice, although an age‐associated increase in serum sIL‐6R level was observed, it was much less extensive than that in MRL/lpr mice. Elevated serum sIL‐6R level at the age of 30 weeks was observed in female and male (NZB × NZW)F1 mice (32 ± 10 ng/ml and 17 ± 5.0 ng/ml, respectively), and male BXSB/Mpj Yaa mice (42 ± 18 ng/ml), suggesting that elevated serum sIL‐6R in aged mice is one of the characteristics of autoimmune‐prone mice. Quantitative analysis of serum IL‐6 in MRL/lpr revealed that the serum sIL‐6R level correlated well with the serum IL‐6 level. We also showed that sIL‐6R in the sera from MRL/lpr mice could mediate the IL‐6 functions through the IL‐6 signal‐transducing receptor component gpl30, suggesting that elevated production of sIL‐6R may partly contribute to development of autoimmune disease in MRL/lpr mice.

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European Journal of Immunology

Tập 23 Số 5

1078-1082

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