Sequential expression of raffinose synthase and stachyose synthase corresponds to successive accumulation of raffinose, stachyose and verbascose in developing seeds of Lens culinaris Medik.

Altschul, 1990, Basic local alignment search tool, J. Mol. Biol., 215, 403, 10.1016/S0022-2836(05)80360-2 Bachmann, 1995, Metabolism of the raffinose family oligosaccharides in leaves of ajuga-reptans l - intercellular and intracellular compartmentation, Plant Physiol., 109, 991, 10.1104/pp.109.3.991 Bilyeu, 2016, Environmental stability of seed carbohydrate profiles in soybeans containing different alleles of the raffinose synthase 2 (RS2) gene, J. Agric. Food Chem., 64, 1071, 10.1021/acs.jafc.5b04779 Blöchl, 2008, Enzymatic breakdown of raffinose oligosaccharides in pea seeds, Planta, 228, 99, 10.1007/s00425-008-0722-4 Carmi, 2003, Cloning and functional expression of alkaline alpha-galactosidase from melon fruit: similarity to plant SIP proteins uncovers a novel family of plant glycosyl hydrolases, Plant J., 33, 97, 10.1046/j.1365-313X.2003.01609.x Chibbar, 2016, Carbohydrate metabolism, vol. 2, 161 Comfort, 2007, Biochemical analysis of thermotoga maritima GH36 α-galactosidase (TmGalA) confirms the mechanistic commonality of clan GH-D Glycoside hydrolases, Biochemistry, 46, 3319, 10.1021/bi061521n Coon, 1990, Effect of oligosaccharide-free soybean meal on true metabolizable energy and fiber digestion in adult Roosters1, Poultry Sci., 69, 787, 10.3382/ps.0690787 Cummings, 1995, Gastrointestinal effects of food carbohydrate, Am. J. Clin. Nutr., 61, S938, 10.1093/ajcn/61.4.938S Dierking, 2008, Association of a soybean raffinose synthase gene with low raffinose and stachyose seed phenotype, Plant Genome, 1, 10.3835/plantgenome2008.06.0321 Doyle, 1990, Isolation of plant DNA from fresh tissue, Focus, 12, 13 Egert, 2013, Abiotic stress-induced accumulation of raffinose in Arabidopsis leaves is mediated by a single raffinose synthase (RS5, At5g40390), BMC Plant Biol., 13, 218, 10.1186/1471-2229-13-218 Finn, 2014, Pfam: the protein families database, Nucleic Acids Res., 42, D222, 10.1093/nar/gkt1223 Gangola, 2016, Galactinol synthase enzyme activity influences raffinose family oligosaccharides (RFO) accumulation in developing chickpea (Cicer arietinum L.) seeds, Phytochemistry, 125, 88, 10.1016/j.phytochem.2016.02.009 Gangola, 2014, A reliable and rapid method for soluble sugars and RFO analysis in chickpea using HPAEC–PAD and its comparison with HPLC–RI, Food Chem., 154, 127, 10.1016/j.foodchem.2013.12.085 Gangola, 2013, Genotype and growing environment interaction shows a positive correlation between substrates of raffinose family oligosaccharides (RFO) biosynthesis and their accumulation in chickpea (Cicer arietinum L.) seeds, J. Agric. Food Chem., 61, 4943, 10.1021/jf3054033 Haab, 2002, Purification and characterization of the raffinose oligosaccharide chain elongation enzyme, galactan: galactan galactosyltransferase (GGT), from Ajuga reptans leaves, Physiol. Plantarum, 114, 361, 10.1034/j.1399-3054.2002.1140305.x Hitz, 2002, Biochemical and molecular characterization of a mutation that confers a decreased raffinosaccharide and phytic acid phenotype on soybean seeds, Plant Physiol., 128, 650, 10.1104/pp.010585 Hoch, 1999, Purification and characterization of stachyose synthase from lentil (Lens culinaris) seeds: galactopinitol and stachyose synthesis, Arch. Biochem. Biophys., 366, 75, 10.1006/abbi.1999.1212 Johnson, 2013, Lentil (Lens culinaris L.): a prebiotic-rich whole food legume, Food Res. Int., 51, 107, 10.1016/j.foodres.2012.11.025 Kannan, 2014, Hexadecyltrimethylammonium bromide (CTAB)-based protocol to isolate high-quality RNA in adequate quantities for gene expression analyses in developing seeds of lentils (Lens culinaris Medik.), Gene Expr. Genet. Genom., 7 Kannan, 2016, Differential expression of two galactinol synthase isoforms LcGolS1 and LcGolS2 in developing lentil (Lens culinaris Medik. cv CDC Redberry) seeds, Plant Physiol. Biochem., 108, 422, 10.1016/j.plaphy.2016.08.004 Kaur, 2011, Transcriptome sequencing of lentil based on second-generation technology permits large-scale unigene assembly and SSR marker discovery, BMC Genom., 12, 10.1186/1471-2164-12-265 Kumar, 2010, Sucrose and raffinose family oligosaccharides (RFOs) in soybean seeds as influenced by genotype and growing location, J. Agric. Food Chem., 58, 5081, 10.1021/jf903141s Lehle, 1973, The function of myo-inositol in the biosynthesis of raffinose. Purification and characterization of galactinol:sucrose 6-galactosyltransferase from Vicia faba seeds, Eur. J. Biochem., 38, 103, 10.1111/j.1432-1033.1973.tb03039.x Livak, 2001, Analysis of relative gene expression data using real-time quantitative PCR and the 2-[delta][delta]CT method, Methods, 25, 402, 10.1006/meth.2001.1262 Nishizawa, 2008, Galactinol and raffinose constitute a novel function to protect plants from oxidative damage, Plant Physiol., 147, 1251, 10.1104/pp.108.122465 Peterbauer, 2003, Enzymatic control of the accumulation of verbascose in pea seeds, Plant Cell Environ., 26, 1385, 10.1046/j.0016-8025.2003.01063.x Peterbauer, 2001, Analysis of the raffinose family oligosaccharide pathway in pea seeds with contrasting carbohydrate composition, Plant Physiol., 127, 1764, 10.1104/pp.010534 Peterbauer, 2002, Functional expression of a cDNA encoding pea (Pisum sativum L.) raffinose synthase, partial purification of the enzyme from maturing seeds, and steady-state kinetic analysis of raffinose synthesis, Planta, 215, 839, 10.1007/s00425-002-0804-7 Peterbauer, 2002, Chain elongation of raffinose in pea seeds - isolation, charcterization, and molecular cloning of a multifunctional enzyme catalyzing the synthesis of stachyose and verbascose, J. Biol. Chem., 277, 194, 10.1074/jbc.M109734200 Peterbauer, 1999, Stachyose synthesis in seeds of adzuki bean (Vigna angularis): molecular cloning and functional expression of stachyose synthase, Plant J., 20, 509, 10.1046/j.1365-313X.1999.00618.x Peterbauer, 1998, Galactosylononitol and stachyose synthesis in seeds of adzuki bean - purification and characterization of stachyose synthase, Plant Physiol., 117, 165, 10.1104/pp.117.1.165 Peters, 2010, Functional identification of arabidopsis ATSIP2 (At3g57520) as an alkaline alpha-galactosidase with a substrate specificity for raffinose and an apparent sink-specific expression pattern, Plant Cell Physiol., 51, 1815, 10.1093/pcp/pcq127 Qiu, 2015, Identification and characterization of a stachyose synthase gene controlling reduced stachyose content in soybean, Theor. Appl. Genet., 128, 2167, 10.1007/s00122-015-2575-0 Ramsay, 2016 Sanderson, 2019, KnowPulse: a web-resource focused on diversity data for pulse crop improvement, Front. Plant Sci., 10, 10.3389/fpls.2019.00965 Sievers, 2011, Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega, Mol. Syst. Biol., 7, 539, 10.1038/msb.2011.75 Skoneczka, 2009, Identification of candidate gene mutation associated with low stachyose phenotype in soybean line PI200508, Crop Sci., 49, 247, 10.2135/cropsci2008.07.0403 Tahir, 2011 Tahir, 2011, Composition and correlation between major seed constituents in selected lentil (Lens culinaris Medik) genotypes, Can. J. Plant Sci., 91, 825, 10.4141/cjps2011-010 Tahir, 2011, Influence of environment on seed soluble carbohydrates in selected lentil cultivars, J. Food Compos. Anal., 24, 596, 10.1016/j.jfca.2010.04.007 Tamura, 2013, MEGA6: molecular evolutionary genetics analysis version 6.0, Mol. Biol. Evol., 30, 2725, 10.1093/molbev/mst197 Tanner, 1967, Myo-inositol, a cofactor in the biosynthesis of verbascose, Biochem. Biophys. Res. Commun., 29, 166, 10.1016/0006-291X(67)90581-5 Tapernoux-Luthi, 2004, Cloning, functional expression, and characterization of the raffinose oligosaccharide chain elongation enzyme, galactan : galactan galactosyltransferase, from common bugle leaves, Plant Physiol., 134, 1377, 10.1104/pp.103.036210 Tosh, 2010, Dietary fibres in pulse seeds and fractions: characterization, functional attributes, and applications, Food Res. Int., 43, 450, 10.1016/j.foodres.2009.09.005 Vandenberg, 2006, CDC Redberry lentil, Can. J. Plant Sci., 86, 497, 10.4141/P05-071 Vandesompele, 2002, Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes, Genome Biol., 3, 10.1186/gb-2002-3-7-research0034 Zhang, 2020, Alternative polyadenylation of the stacyose synthase gene mediates source-sink regulation in cucumber, J. Plant Physiol., 245, 153111, 10.1016/j.jplph.2019.153111 Zhu, 1994, Cloning and functional expression of a cDNA encoding coffee bean α-galactosidase, Gene, 140, 227, 10.1016/0378-1119(94)90548-7