Sequence variability in two mitochondrial DNA regions and internal transcribed spacer among three cestodes infecting animals and humans from China

Journal of Helminthology - Tập 86 Số 2 - Trang 245-251 - 2012
R.S. Dai1,2, G.H. Liu1,2, Hui‐Qun Song3,2, Rui-Qing Lin3,2, Zi‐Guo Yuan3, Mengxian Li4, Si‐Yang Huang2, Wei Liu1,2, Xing‐Quan Zhu5,2
1College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province410128, PR China
2State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, Gansu Province730046, PR China
3College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province510642, PR China
4Department of Veterinary Medicine, Guangdong Ocean University, Zhanjiang, Guangdong Province524088, PR China
5College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province650201, PR China

Tóm tắt

Abstract

Sequence variability in two mitochondrial DNA (mtDNA) regions, namely cytochromecoxidase subunit 1 (cox1) and NADH dehydrogenase subunit 4 (nad4), and internal transcribed spacer (ITS) of rDNA among and within three cestodes,Spirometra erinaceieuropaei,Taenia multicepsandTaenia hydatigena, from different geographical origins in China was examined. A portion of thecox1 (pcox1),nad4 genes (pnad4) and the ITS (ITS1+5.8S rDNA+ITS2) were amplified separately from individual cestodes by polymerase chain reaction (PCR). Representative amplicons were subjected to sequencing in order to estimate sequence variability. While the intra-specific sequence variations within each of the tapeworm species were 0–0.7% for pcox1, 0–1.7% for pnad4 and 0.1–3.6% for ITS, the inter-specific sequence differences were significantly higher, being 12.1–17.6%, 18.7–26.2% and 31–75.5% for pcox1, pnad4 and ITS, respectively. Phylogenetic analyses based on the pcox1 sequence data revealed thatT. multicepsandT. hydatigenawere more closely related to the other members of theTaeniagenus, andS. erinaceieuropaeiwas more closely related to the other members of theSpirometragenus. These findings demonstrated clearly the usefulness of mtDNA and rDNA sequences for population genetic studies of these cestodes of socio-economic importance.

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Thông tin xuất bản

Journal of Helminthology

Tập 86 Số 2

245-251

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