Sequence comparison of the 5’ flanking regions of Japanese pear (Pyrus pyrifolia) S-RNases associated with gametophytic self-incompatibility

Springer Science and Business Media LLC - Tập 13 - Trang 289-291 - 2001
Naoko Norioka1, Hironori Katayama2, Tohru Matsuki1, Takeshi Ishimizu3, Takeshi Takasaki4, Tetsu Nakanishi4, S. Norioka1
1Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan e-mail: [email protected] Tel.: +816-6879-8618, Fax: +816-6879-8619, , JP
2Experimental Farm, Kobe University, 1348 Uzurano, Kasai, Hyogo 657-2103, Japan, , JP
3Graduate School of Science, Osaka University, Osaka 560-0043, Japan, , JP
4Faculty of Agriculture, Kobe University, Hyogo 657-8501, Japan, , JP

Tóm tắt

Genomic clones of 2.8 kb, 4.3 kb and 6.5 kb for the S2-, S3- and S5-RNases of Japanese pear(Pyrus pyrifolia), respectively, were isolated and sequenced. Comparison of the 5’-flanking regions of these genes with the same region of the S4-RNase gene indicated that a highly similar region of approximately 200 bp exists in the regions just upstream of the putative TATA boxes of the four Japanese pear S-RNase genes. This suggests the presence of cis-regulatory element(s) in this region.