Separation of ketoprofen enantiomers at nanomolar concentration levels by micellar electrokinetic chromatography with on-line electrokinetic preconcentration

Walter de Gruyter GmbH - Tập 11 Số 3 - Trang 335-340 - 2013
Jan Petr1, Pavlína Ginterová1, Joanna Znaleziona1, Radim Knob2, Markéta Lošťáková3, Vítězslav Maier1, Juraj Ševčı́k2
1Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University in Olomouc, CZ-77146, Olomouc, Czech Republic
2Department of Analytical Chemistry, Faculty of Science, Palacký University in Olomouc, CZ-77146, Olomouc, Czech Republic
3Southern Älvsborg Hospital, SE-50182, Borås, Sweden

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Abstract Abstract Separation of enantiomers represents an extremely important task in the field of analytical chemistry. This paper contributes to the field of the on-line preconcentration of enantiomers by developing a novel setup based on the electrokinetic accumulation of ketoprofen enantiomers on the pH boundary followed by enantioselective mobilization by a mixture of SDS, sulfated-β-cyclodextrin (S-β-CD), and trimethyl-β-cyclodextrin (TM-β-CD). Under the best conditions, where the injection electrolyte was composed of 50 mmol L−1 borate/NaOH pH 9.5 with 60% (v/v) methanol, the background electrolyte contained 50 mmol L−1 phosphate/NaOH pH 2.5, and the mobilization electrolyte consisted of 50 mmol L−1 phosphate/NaOH pH 2.5 with 4.0% (w/v) S-β-CD, 0.5% (w/v) TM-β-CD, and 20 mmol L−1 SDS, the determination of nanomolar concentration levels of ketoprofen enantiomers was successful by using micellar electrokinetic chromatography with a common UV detection. LODs were 2.5 nmol L−1 and 3.4 nmol L−1, which represent enhancement factors of 9921 and 8529, respectively. The method was also applied to the determination of ketoprofen enantiomers in waste water samples by using simple filtration as a clean-up step. Here, the recovery of ketoprofen enantiomers was 91% at the concentration level of 5×10−9 mol L−1. Graphical abstract

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Walter de Gruyter GmbH

Tập 11 Số 3

335-340

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