Sensitive fluorescent imaging of Salmonella enteritidis and Salmonella typhimurium using a polyvalent directed peptide polymer

Microchimica Acta - Tập 184 - Trang 2611-2620 - 2017
Sang-Choon Lee1, Min-Seo Kim1, Ki-Chun Yoo2, Na-Reum Ha1, Ji-Young Moon1, Su-Jae Lee2, Moon-Young Yoon1
1Department of Chemistry and Research Institute for Natural Sciences, Hanyang University, Seoul, Republic of Korea
2Department of Life Science, Research Institute for Natural Sciences, Hanyang University, Seoul, Republic of Korea

Tóm tắt

The authors describe three fluorescein-conjugated peptides generated by cell-phage display for use as a diagnostic probes for fluorescent detection and imaging of Salmonella enteritidis and Salmonella typhimurium. The authors also designed a polyvalent-directed peptide polymer synthesized with poly-D-lysine and bifunctional succinimidyl 3-(2-pyridyldithio)propionate with an affinity and sensitivity that is higher by more than an order of magnitude compared to single peptides due to multiple binding site interactions. In order to establish a diagnostic system for food poisoning, imaging analysis was performed using fluorescence microscopy. The limit of detection of the diagnostic system based on polyvalent directed peptide interaction is 102 colony-forming units per mL for Salmonella.

Tài liệu tham khảo

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