Sensitive detection of EGFR mutations using a competitive probe to suppress background in the SMart Amplification Process

Miller, 2005, High-density single-nucleotide polymorphism maps of the human genome, Genomics, 86, 117, 10.1016/j.ygeno.2005.04.012 Human Genome Sequencing Consortium, 2004, Finishing the euchromatic sequence of the human genome, Nature, 431, 931, 10.1038/nature03001 Sachidanandam, 2001, A map of human genome sequence variation containing 1.42 million single nucleotide polymorphisms, Nature, 409, 928, 10.1038/35057149 Mitani, 2007, Rapid SNP diagnostics using asymmetric isothermal amplification and a new mismatch-suppression technology, Nat Methods, 4, 257, 10.1038/nmeth1007 Biswas, 1996, Identification and characterization of a thermostable MutS homolog from Thermus aquaticus, J Biol Chem, 271, 5040, 10.1074/jbc.271.9.5040 Whitehouse, 1997, Analysis of the mismatch and insertion/deletion binding properties of Thermus thermophilus, HB8, MutS, Biochem Biophys Res Commun, 233, 834, 10.1006/bbrc.1997.6551 Lynch, 2004, Activating mutations in the epidermal growth factor receptor underlying responsiveness of non-small-cell lung cancer to gefitinib, N Engl J Med, 350, 2129, 10.1056/NEJMoa040938 Paez, 2004, EGFR mutations in lung cancer: correlation with clinical response to gefitinib therapy, Science, 304, 1497, 10.1126/science.1099314 Pao, 2004, EGF receptor gene mutations are common in lung cancers from “never smokers” and are associated with sensitivity of tumors to gefitinib and erlotinib, Proc Natl Acad Sci U S A, 101, 13306, 10.1073/pnas.0405220101 Maruyama, 2007, Detection of epidermal growth factor receptor (EGFR) mutations in non-small cell lung cancer (NSCLC) using a fully automated system with a nano-scale engineered biomagnetite, Biosens Bioelectron, 22, 2282, 10.1016/j.bios.2006.11.018 Watanabe, 2007, Use of a competitive probe in assay design for genotyping of the UGT1A1 *28 microsatellite polymorphism by the smart amplification process, Biotechniques, 43, 479, 10.2144/000112563 Castoldi, 2006, A sensitive array for microRNA expression profiling (miChip) based on locked nucleic acids (LNA), RNA, 12, 913, 10.1261/rna.2332406 Chiou, 2006, Single-tube reaction using peptide nucleic acid as both PCR clamp and sensor probe for the detection of rare mutations, Nat Protoc, 1, 2604, 10.1038/nprot.2006.428