Sensitive and specific detection of Xanthomonas campestris pv. campestris by PCR using species-specific primers based on hrpF gene sequences

Alvarez, 1985, Rapid identification of Xanthomonas campestris pv. campestris by ELISA, Plant Dis., 69, 1082, 10.1094/PD-69-1082 Bassam, 1992, DNA amplification fingerprinting of bacteria, Appl. Microbiol. Biotechnol., 38, 70, 10.1007/BF00169422 Chitarra, 2002, Detection of the plant pathogenic bacterium Xanthomonas campestris pv. campestris in seed extracts of Brassica sp. applying fluorescent antibodies and flow cytometry, Cytometry, 47, 118, 10.1002/cyto.10058 Hartung, 1993, Detection of Xanthomonas campestris pv. citri by the polymerase chain reaction method, Appl. Environ. Microbiol., 59, 1143, 10.1128/AEM.59.4.1143-1148.1993 Hayward, 1993, The host of Xanthomonas, 51 Lazo, 1987, Conservation of plasmid DNA sequences and pathovar identification of strains of Xanthomonas campestris, Phytopathology, 77, 448, 10.1094/Phyto-77-448 Leite, 1994, Detection and identification of phytopathogenic Xanthomonas strains by amplification of DNA sequences related to the hrp genes of Xanthomonas campestris pv. vesicatoria, Appl. Environ. Microbiol., 60, 1068, 10.1128/AEM.60.4.1068-1077.1994 Murray, 1980, Rapid isolation of high molecular-weight plant DNA, Nucl. Acids Res., 8, 4321, 10.1093/nar/8.19.4321 Rasmussen, 1992, DNA probes for the detection of plant pathogenic bacteria, J. Biotechnol., 25, 203, 10.1016/0168-1656(92)90156-4 Rossier, 2000, HrpB2 and HrpF from Xanthomonas are type III-secreted proteins and essential for pathogenicity and recognition by the host plant, Mol. Microbiol., 38, 828, 10.1046/j.1365-2958.2000.02173.x Sambrook, 1989 Schierwater, 1993, Different thermostable DNA polymerases may amplify different RAPD products, Nucl. Acid Res., 21, 4647, 10.1093/nar/21.19.4647 Viprey, 1998, Symbiotic implications of type III protein secretion machinery in Rhizobium, Mol. Microbiol., 28, 1381, 10.1046/j.1365-2958.1998.00920.x