Selective spectrofluorimetric method for the determination of perindopril erbumine in bulk and tablets through derivatization with dansyl chloride

Journal of Analytical Science and Technology - Tập 6 Số 1 - 2015
Amir Alhaj Sakur1, Tamim Chalati2, Hanan Fael1
1Department of Analytical Chemistry, Faculty of Pharmacy, University of Aleppo, Aleppo, Syria
2Department of Pharmaceutics, Faculty of Pharmacy, University of Aleppo, Aleppo, Syria

Tóm tắt

Abstract Background Perindopril erbumine is an antihypertensive, which belongs to the category of angiotensin-converting enzyme inhibitors (ACE inhibitors) that inhibit the conversion of angiotensin I to angiotensin II. Methods A new, selective, and sensitive spectrofluorimetric method was developed for the determination of perindopril erbumine based on the reaction with dansyl chloride in alkaline medium to give a highly fluorescent derivative which was measured at 496 nm after excitation at 340 nm in dichloromethane. The reaction conditions were studied and optimized. Results Under the optimum conditions, the fluorescence intensity was linear over a concentration range of 1.0 to 21.0 μg/mL (R 2 = 0.9997) with a detection limit of 0.242 μg/mL. In order to validate the method, the results were compared with those obtained by a high performance liquid chromatography method. Conclusions The proposed method was successfully applied to the analysis of perindopril erbumine in pure form and tablets with good precision and accuracy as revealed by t- and F tests. The mechanism of the reaction has also been discussed.

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Tài liệu tham khảo

Abd El Ghaffar ME, El Wasseef DR, El Sherbiny DT, El Ashry SM (2011) Spectrofluorimetric determination of two β agonist drugs in bulk and pharmaceutical dosage forms via derivatization with dansyl chloride. J Anal Chem 66:476–481

Abdel Fattah LS, Mohamed TA, Taha EA (2010) Spectrofluorimetric determination of carvedilol in dosage form and spiked human plasma through derivatization with 1-dimethylaminonaphthalene-5-sulphonyl chloride. Chem Ind Chem Eng Q 16:31–38

Aydoğmuş Z, Sarı F, Ulu ST (2012) Spectrofluorimetric determination of aliskiren in tablets and spiked human plasma through derivatization with dansyl chloride. J Fluoresc 22:549–556

Bartzatt R (2003) Dansylation of aromatic, aliphatic and medicinal carboxylic acid compounds in 1 M Na2CO3 buffer. Anal Chim Acta 488:203–209

Joint Formulary Committee (2014) British National Formulary, 67th edition. British Medical Association and Royal Pharmaceutical Society of Great Britain, London

Chaudhary AB, Patel RK, Chaudhary SA (2010) Determination of losartan potassium and perindopril erbumine in tablet formulations by reversed-phase HPLC. International Journal of ChemTech Research 2:1141–1146

Dewani MG, Bothara KG, Madgulkar AR, Damle MC (2011) Simultaneous estimation of perindopril erbumine and indapamide in bulk drug and tablet dosage form by HPTLC. International Journal of Comprehensive Pharmacy 2:1–4

Harvey D (2009). Modern analytical chemistry. Second ed., David Harvey.

Jaina DS, Subbaiah G, Sanya M, Pande UC, Shrivastav P (2006) First LC-MS/MS electrospray ionization validated method for the quantification of perindopril and its metabolite perindoprilat in human plasma and its application to bioequivalence study. J Chromatogr B 837:92–100

Jogia H, Khandelwal U, Gandhi T, Singh S, Modi D (2010) Development and validation of a stability-indicating assay method for simultaneous determination of perindopril and indapamide in combined dosage form by reversed-phase high-performance liquid chromatography. J AOAC Int 93:108–115

Joseph J, Philip B, Sundarapandian M (2011) Method development and validation for simultaneous estimation of perindopril erbumine and indapamide by RP-HPLC in pharmaceutical dosage forms. International Journal of Pharmacy and Pharmaceutical Sciences 3:288–293

Karasakal A, Ulu ST (2013) New spectrofluorimetric method for the determination of nizatidine in bulk form and in pharmaceutical preparations. Opt Spectrosc 115:306–309

Neelam S, Gopisetti J, Chandra BS (2012) Spectrophotometric analysis of perindopril erbumine in bulk and tablets using bromophenol blue. Der Pharmacia Lettre 4:159–169

Nirogi RVS, Kandikere VN, Shukla M, Mudigonda K, Maurya S, Komarneni P (2006) High-throughput quantification of perindopril in human plasma by liquid chromatography/tandem mass spectrometry: application to a bioequivalence study. Rapid Commun Mass Spectrom 20:1864–1870

Prajapati J, Patel A, Patel MB, Prajapati N, Prajapati R (2011a) Analytical method development and validation of amlodipine besylate and perindopril erbuminee in combine dosage form by RP-HPLC. International Journal of PharmTech Research 3:801–808

Prajapati J, Patel MB, Prajapati N, Prajapati R (2011b) Simultaneous determination of perindopril erbumine and amlodipine besylate by absorption factor method. Int J Appl Biol Pharmaceut Tech 2:230–233

Rahman N, Rahman H, Khatoon A (2012) Development of spectrophotometric method for the determination of perindopril erbumine in pharmaceutical formulations using 2, 4 dinitrofluorobenzene. J Chil Chem Soc 57:1069–1073

Raju VB, Rao AL (2011) Development and validation of new HPLC method for the estimation of perindopril in tablet dosage forms. Rasayan J Chem 4:113–116

Riyaz SMD, Vasanth PM, Ramesh M, Ramesh R, Ramesh T (2012) A sensitive RP-HPLC method development and validation for the simultaneous estimation of perindopril erbumine and amlodipine besilate in tablet dosage form. International journal of chemical and life science 1:1033–1038

Ulu ST (2011) Sensitive spectrofluorimetric determination of tizanidine in pharmaceutical preparations, human plasma and urine through derivatization with dansyl chloride. Luminescence: The Journal of Biological and Chemical Luminescence. doi:10.1002/bio.1367

Zaazaa HE, Abbas SS, Essam HA, El Bardicy MG (2013) Validated chromatographic methods for determination of perindopril and amlodipine in pharmaceutical formulation in the presence of their degradation products. J Chromatogr Sci 51:533–543

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Journal of Analytical Science and Technology

Tập 6 Số 1

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