Selective and powerful stress gene expression in <i>Arabidopsis</i> in response to malondialdehyde

Plant Journal - Tập 37 Số 6 - Trang 877-888 - 2004
Hans Weber1, Aurore Chételat1, Philippe Reymond1, Edward E. Farmer
1Gene Expression Laboratory, Plant Molecular Biology, University of Lausanne, Biology Building, 1015 Lausanne, Switzerland.

Tóm tắt

SummaryThe provenance, half‐life and biological activity of malondialdehyde (MDA) were investigated in Arabidopsis thaliana. We provide genetic confirmation of the hypothesis that MDA originates from fatty acids containing more than two methylene‐linked double bonds, showing that tri‐unsaturated fatty acids are the in vivo source of up to 75% of MDA. The abundance of the combined pool of free and reversibly bound MDA did not change dramatically in stress, although a significant increase in the free MDA pool under oxidative conditions was observed. The half‐life of infiltrated MDA indicated rapid metabolic turnover/sequestration. Exposure of plants to low levels of MDA using a recently developed protocol powerfully upregulated many genes on a cDNA microarray with a bias towards those implicated in abiotic/environmental stress (e.g. ROF1 and XERO2). Remarkably, and in contrast to the activities of other reactive electrophile species (i.e. small vinyl ketones), none of the pathogenesis‐related (PR) genes tested responded to MDA. The use of structural mimics of MDA isomers suggested that the propensity of the molecule to act as a cross‐linking/modifying reagent might contribute to the activation of gene expression. Changes in the concentration/localisation of unbound MDA in vivo could strongly affect stress‐related transcription.

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Tài liệu tham khảo

10.1016/0891-5849(94)00096-3

10.1046/j.1365-313X.2003.01718.x

10.1007/s004250050545

10.1146/annurev.cellbio.16.1.1

10.1104/pp.126.3.1024

10.1126/science.274.5293.1715

10.1007/PL00008113

10.1074/jbc.M106838200

Chojkier M., 1989, Stimulation of collagen gene‐expression by ascorbic acid in cultured human‐fibroblasts – a role for lipid‐peroxidation, J. Biol. Chem., 264, 16957, 10.1016/S0021-9258(19)84800-7

10.3109/10715769709065764

10.1046/j.1365-313x.1999.00622.x

10.1126/science.266.5188.1247

10.1016/S0076-6879(99)03014-1

10.1016/0891-5849(91)90192-6

10.1016/S1369-5266(03)00045-1

10.1016/S0168-9525(00)02107-7

10.2307/3869877

10.1074/jbc.M203368200

10.1016/S1369-5266(00)00177-1

10.1016/S0176-1617(98)80179-X

10.1016/S0014-5793(03)00758-0

Guzman P., 1990, Exploiting the triple response of Arabidopsis to identify ethylene‐related mutants, Plant Cell, 2, 513

10.1016/0092-8674(91)90611-2

10.1078/0176-1617-00541

10.1104/pp.119.2.693

Kurganova L.N., 1999, Lipid peroxidation products as possible mediators of heat stress response in plants, Russ. J. Plant Physiol., 46, 181

10.1016/S0300-483X(02)00448-1

10.1105/tpc.8.3.403

10.1007/s004250000401

10.1093/jexbot/53.367.207

10.1016/S0014-5793(02)03041-7

10.1023/A:1006321900483

10.1074/jbc.275.16.11784

10.1021/jo00912a038

10.1105/tpc.12.5.707

10.1016/0014-5793(96)00051-8

Sanchez E.R., 1990, Hsp56 – a novel heat‐shock protein associated with untransformed steroid‐receptor complexes, J. Biol. Chem., 265, 22067, 10.1016/S0021-9258(18)45667-0

10.1007/s001250051342

10.1073/pnas.211311098

10.1093/oxfordjournals.pcp.a029469

10.1046/j.1365-313X.2003.01730.x

10.1128/jb.179.4.1096-1101.1997

10.1016/S1050-1738(99)00016-X

10.1073/pnas.141079498

10.1111/j.1365-313X.2000.00897.x

10.1007/BF02172397

10.1023/A:1015557300450

10.1007/BF00043887

10.1006/abio.1994.1355

10.1016/S0076-6879(99)00115-9

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Plant Journal

Tập 37 Số 6

877-888

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