Screening of Plant Extracts for Antioxidant Activity: a Comparative Study on Three Testing Methods

Phytochemical Analysis - Tập 13 Số 1 - Trang 8-17 - 2002
Irina Koleva1, Teris A. van Beek1, J.P.H. Linssen2, C.P.G.M. de Groot1, L. Evstatieva3
1Laboratory of Organic Chemistry, Wageningen University, Dreijenplein 8, 6703 HB Wageningen, The Netherlands
2Laboratory of Food Chemistry, Department of Agrotechnology and Food Sciences, Wageningen University, PO Box 8129, 6700 EV Wageningen, The Netherlands
3Institute of Botany, Bulgarian Academy of Sciences, Sofia, Bulgaria

Tóm tắt

AbstractThree methods widely employed in the evaluation of antioxidant activity, namely 2,2′‐diphenyl‐1‐picrylhydrazyl (DPPH) radical scavenging method, static headspace gas chromatography (HS‐GC) and β‐carotene bleaching test (BCBT), have been compared with regard to their application in the screening of plant extracts. The strengths and limitations of each method have been illustrated by testing a number of extracts, of differing polarity, from plants of the genus Sideritis, and two known antioxidants (butylated hydroxytoluene and rosmarinic acid). The sample polarity was important for the exhibited activity in the BCBT and HS‐GC methods but not for the DPPH method. The complex composition of the extracts and partition phenomena affected their activity in each assay. The value of the BCBT method appears to be limited to less polar samples. Although slow, the HS‐GC method is preferable for assessing the antioxidant inhibitory properties on the formation of unwanted secondary volatile products. Being rapid, simple and independent of sample polarity, the DPPH method is very convenient for the quick screening of many samples for radical scavenging activity. Copyright © 2001 John Wiley & Sons, Ltd.

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Phytochemical Analysis

Tập 13 Số 1

8-17

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