Screening for Differential Gene Expression in Atropa belladonna Leafy Gall Induced Following Rhodococcus fascians Infection
Tóm tắt
To better characterise at the molecular level the nature of plant responses to infection by Rhodococcus fascians PCR-based differential display patterns of Atropa belladonna leafy gall (LG) and non-infected plant tissues were compared. Six differentially expressed genes were identified and their altered expression was confirmed by RT-PCR. Three of them corresponded to up-regulated genes which encode proteins involved in plant defence. The three remaining cDNA fragments which correspond to down-regulated genes in LG, encoded proteins with similarity to a multicystatin, a miraculin and a methallothionein-like protein, respectively. Upon elimination of the bacteria from infected plant tissue, the expression of up-regulated genes was maintained, whereas expression of down-regulated genes resumed suggesting a potential role of these up-regulated genes in plant growth and development.
Tài liệu tham khảo
Botella MA, Xu Y, Prabha TN, Zhao Y, Narasimhan ML, Wilson KA, Nielsen SS, Bressan RA and Hasegawa PM (1996) Differential expression of soybean cysteine proteinase inhibitor genes during development and in response to wounding and methyl jasmonate. Plant Physiology 112: 1201-1210
Brenner ED, Lambert KN, Kaloshian I and Williamson VM (1998) Characterization of LeMir, a root-knot nematode-induced gene in tomato with an encoded product secreted from the root. Plant Physiology 118: 237-247
Choi D, Kim HN, Yun HK, Park JA, Kim TW and Bok SH (1996) Molecular cloning of a metallothionein-like gene from Nicotiana glutinosa L. and its induction by wounding and tobacco mosaic virus infection. Plant Physiology 112: 353-359
de-Castro LA, Carneiro M, Neshich DC and de Paiva GR (1992) Spatial and temporal gene expression patterns occur during corn development. Plant Cell 4: 1549-1559
De Haan M, Maarse AC, Grivell LA, Bancroft I, Newes HW, Mayer K, Schueller C and Bevan M (1998) EU Arabidopsis sequence project. Direct submission: CAA19698
Goethals K, Vereecke D, Jaziri M, Van Montagu M and Holsters M (2001) Leafy gall formation by Rhodococcus fascians. Annual Review Phytopathology 39: 27-52
Hsieh HM, Liu WK and Huang PC (1995) A novel stressinducible metallothionein-like gene from rice. Plant Molecular Biology 28: 381-389
Jakobek JL, Smith JA and Lindgren PB (1993) Suppression of bean defence responses by Pseudomonas syringae. Plant Cell 5: 57-63
Jaziri M, Goethals K and Van Montagu M (1998) Plant micropropagation and germplasm storage. Patent WO 98/36635
Jin-Zhou D and Dunstan DI (1996) Expression of abundant mRNAs during somatic embryogenesis of white spruce (Picea glauca (Moench) Voss). Planta 199: 459-466
Liang P, Zhu W, Zhang X, Guo Z, O'Connell RP, Averboukh L, Wang F and Pardee B (1994) Differential display using onebase anchored oligo-dT primers. Nucleic Acids Research 22: 5763-5764
Milligan SB and Gasser CS (1995) Nature and regulation of pistil-expressed genes in tomato. Plant Molecular Biology 28: 691-711
Moriguchi T, Kita M, Hisada S, Endo-Inagaki T and Omura M (1998) Characterization of gene repertoires at mature stage of citrus fruits through random sequencing and analysis of redundant matallothionein-like genes expressed during fruit development. Gene 211: 221-227
de O Manes CL, Van Montagu M, Prinsen E, Goethals K and Holsters M (2001) De novo cortical cell division triggered by the phytopathogen Rhodococcus fascians in tobacco. Molecular Plant-Microbe Interactions 14: 189-195
Okushima Y, Koizumi N, Kusano T and Sano H (2000) Secreted proteins of tobacco cultured BY2 cells: Identification of a new member of pathogenesis-related proteins. Plant Molecular Biology 42: 479-488
Pautot V, Holzer FM and Walling LL (1991) Differential expression of tomato proteinase inhibitor I and II genes during bacterial pathogen invasion and wounding. Molecular Plant-Microbe Interactions 4: 284-292
Ryan SN, Laing WA and McManus MT (1998) A cysteine proteinase inhibitor purified from apple fruit. Phytochemistry 49: 957-963
Shinshi H, Wenzler H, Neuhaus JM, Felix G, Hofsteenge J and Meins FJ (1988) Evidence for N-and C-terminal processing of a plant defence-related enzyme: The primary structure of tobacco prepo-beta-1,3-glucanase. Proceeding of the National Academy of Science USA 85: 5541-5545
Solomon M, Belenghi B, Delledonne M, Menachem E and Levine A (1999) The involvement of cysteine proteinase and protease inhibitor genes in the regulation of programmed cell death in plants. The Plant Cell 11: 431-443
Vereecke D, Burssens S, Simon-Mateo C, Inzé D, Van Montagu M, Goethals K and Jaziri M (2000) The Rhodococcus fascians-plant interaction: Morphological traits and biotechnological applications. Planta 210: 241-251
Waldron C, Wegrich LM, Merlo PA and Walsh TA (1993) Characterization of a genomic sequence coding for potato multicystatin, an eight-domain cystein proteinase inhibitor. Plant Molecular Biology 23: 801-812
Whitelaw CA, Le-Huquet JA, Thurman DA, Tomsett AB (1997) The isolation and characterization of type II metallothioneinlike genes from tomato (Lycopersicon esculentum L.). Plant Molecular Biology 33: 503-511
Zhou J and Goldsbrough PB (1995) Structure, organisation and expression of the metallothionein gene family in Arabidopsis. Molecular and Generel Genetics 248: 318-328