Screening Assay for the Detection of the Protein-Protein Interaction Between HIV-1 Nef Protein and the SH3 Domain of Hck

Mayer, 1993, Signalling through SH2 and SH3 domains, Trends Cell Biol, 3, 8, 10.1016/0962-8924(93)90194-6 Deacon, 1995, Genomic structure of an attenuated quasi species of HIV-1 from a blood transfusion donor and recipients, Science, 270, 988, 10.1126/science.270.5238.988 Skinner, 1994, Direct measurement of the binding of RAS to neurofibromin using a scintillation proximity assay, Anal. Biochem, 223, 258, 10.1006/abio.1994.1582 Viguera, 1994, Characterization of the interaction of natural proline-rich peptides with five different SH3 domains, Biochemistry, 33, 10925, 10.1021/bi00202a011 Lee, 1996, Crystal structure of the conserved core of HIV-1 Nef complexed with a Src-family SH3 domain, Cell, 85, 931, 10.1016/S0092-8674(00)81276-3 Daniel, 1992, Protective effects of live attenuated SIV vaccine with a deletion in the nef gene, Science, 258, 1938, 10.1126/science.1470917 Saksela, 1995, Proline-rich (PxxP) motifs in HIV-1 nef bind to SH3 domains of a subset of Src kinases and are required for the enhanced growth of Nef viruses but not for down-regulation of CD4, Embo J., 14, 484, 10.1002/j.1460-2075.1995.tb07024.x Bosworth, 1989, Scintillation proximity assay, Nature, 341, 167, 10.1038/341167a0 Yu, 1994, Structural basis for the binding of prolinerich peptides to SH3 domains, Cell, 76, 933, 10.1016/0092-8674(94)90367-0 Lee, 1995, A single amino acid in the SH3 domain of Hck determines its high affinity in binding to HIV-1 Nef protein, Embo J., 14, 5006, 10.1002/j.1460-2075.1995.tb00183.x Quintrell, 1987, Identification of a Human gene (hck) that encodes a protein-tyrosine kinase and is expressed in hematopoietic cells, Mol. Cell. Biol., 7, 2267 Ziegler, 1987, Novel protein-tyrosine kinase gene (hck) preferentially expressed in cells of the hematopoietic origin, Mol. Cell. Biol., 7, 2276 Kock, 1991, SH2 and SH3 domains: Elements that control interactions of cytoplasmic signalling proteins, Science, 252, 668, 10.1126/science.1708916 Pawson, 1992, SH2 and SH3 domains: From structure to function, Cell, 72, 359, 10.1016/0092-8674(92)90504-6 Du, 1995, Identification of a nef allele that causes lymphocyte activation and acute disease in macaque monkeys, Cell, 82, 665, 10.1016/0092-8674(95)90038-1 Holtzman, 1987, Isolation and sequence of a cDNA corresponding to a src-related gene expressed in murine hemopoietic cells, Proc. Natl. Acad. Sci., 84, 8325, 10.1073/pnas.84.23.8325 Kestler, 1991, Importance of the nef gene for the maintenance of high viral loads and/or development of AIDS, Cell, 65, 651, 10.1016/0092-8674(91)90097-I