Xác thực khoa học về khả năng kháng khuẩn và chống sinh sản của vỏ rễ cây Berberis aristata DC, các thành phần thực vật của nó và độ an toàn sinh học của chúng

AMB Express - 2019
Henna Sood1, Yashwant Kumar2, Vipan Gupta3, Daljit Singh Arora1
1Microbial Technology Laboratory, Department of Microbiology, Guru Nanak Dev University, Amritsar 143005, India
2National Salmonella & Escherichia Centre and Diagnostic Reagents Laboratory, Central Research Institute, Kasauli, H.P., 173204, India
3Department of Veterinary Pathology, Dr. G.C.Negi College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur, H.P., 176062, India

Tóm tắt

Tóm tắt Berberis aristata là một phần quan trọng trong hệ thống chữa bệnh truyền thống từ hơn 2500 năm. Chiết xuất nước từ vỏ rễ Berberis aristata cho thấy hoạt tính rộng rãi chống lại 13 tác nhân gây bệnh thử nghiệm, dao động từ 12 đến 25 mm. Trong tối ưu hóa cổ điển, nồng độ 15% được chuẩn bị ở 40 °C trong 40 phút là tối ưu và ổn định nhiệt. Tối ưu hóa thống kê đã tăng cường hoạt tính từ 1.13 đến 1.30 lần. Ethyl acetate là dung môi hữu cơ tốt nhất để loại bỏ hợp chất tiềm năng chịu trách nhiệm cho hoạt động kháng khuẩn. Diterpenes là thành phần phytoconstituent phong phú nhất (15,3%) và cho thấy hoạt tính kháng khuẩn rộng rãi dao động từ 16,66 đến 42,66 mm. Chiết xuất ethyl acetate hiển thị nồng độ ức chế tối thiểu thấp nhất (0,05–1 mg/mL), theo sau là diterpenes (0,05–5 mg/mL) và flavonoids (0,05–10 mg/mL). Các mẫu chiết xuất thử nghiệm có tính vi khuẩn tiêu diệt và cho thấy tác động kéo dài sau kháng sinh kéo dài từ 2 đến 8 giờ. Chúng được tìm thấy là an toàn sinh học theo thử nghiệm Ames và MTT. Đánh giá độc tính tế bào in vitro của diterpenes đối với các dòng tế bào L20B, RD và Hep 2 cho thấy IC50 dao động từ 245 đến 473 µg/mL. Độc tính cấp tính bằng đường miệng của diterpenes trên chuột Swiss albino không cho thấy bất kỳ thay đổi nào trong mẫu hành vi, trọng lượng cơ thể, các tham số sinh hóa cũng như cấu trúc của các cơ quan. Nghiên cứu này do đó chỉ ra rằng B. aristata có thể là một ứng cử viên tiềm năng cho sự phát triển của thuốc hiệu quả nhờ vào khả năng kháng khuẩn và hồ sơ an toàn sinh học của nó.

Từ khóa


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