STRUCTURING THE UNIVERSE OF PROTEINS

Annual Review of Genomics and Human Genetics - Tập 3 Số 1 - Trang 243-262 - 2002
S.K. Burley1, J.B. Bonanno1
1Howard Hughes Medical Institute, Laboratories of Molecular Biophysics, The Rockefeller University, New York New York 10021;

Tóm tắt

▪ Abstract  High-throughput sequencing of human genomes and those of important model organisms (mouse, Drosophila melanogaster, Caenorhabditis elegans, fungi, archaea) and bacterial pathogens has laid the foundation for another “big science” initiative in biology. Together, X-ray crystallographers, nuclear magnetic resonance (NMR) spectroscopists, and computational biologists are pursuing high-throughput structural studies aimed at developing a comprehensive three-dimensional view of the protein structure universe. The new science of structural genomics promises more than 10,000 experimental protein structures and millions of calculated homology models of related proteins. The evolutionary underpinnings and technological challenges of automating target selection, protein expression and purification, sample preparation, NMR and X-ray data measurement/analysis, homology modeling, and structure/function annotation are discussed in detail. An informative case study from one of the structural genomics centers funded by the National Institutes of Health and the National Institute of General Medical Sciences (NIH/NIGMS) demonstrates how this experimental/computational pipeline will reveal important links between form and function in biology and provide new insights into evolution and human health and disease.

Từ khóa


Tài liệu tham khảo

10.1038/80754

10.1093/nar/25.17.3389

10.1126/science.1065659

10.1126/science.289.5481.905

10.1093/nar/28.1.235

10.1093/nar/29.13.2884

10.1016/S0079-6107(00)00008-0

10.1073/pnas.181466998

10.1002/pro.5560020714

10.1093/nar/27.1.251

10.1016/S0959-440X(97)80054-1

10.1110/ps.9.1.197

Brünger AT, 1998, Acta Crystallogr., 54, 905, 10.1107/S0108767398011465

10.1038/13783

Carter CW, 1979, J. Biol. Chem., 254, 12219, 10.1016/S0021-9258(19)86452-9

10.1110/ps.4570102

10.1016/S0969-2126(96)00108-6

10.1128/JB.183.1.292-300.2001

10.1038/nsb1101-953

10.1093/nar/29.1.55

10.1038/80751

10.1016/S1360-1385(00)01812-4

10.1016/S0959-440X(00)00154-8

Ferre-D'Amare AR, Burley SK. 1995. Dynamic light scattering as a tool for evaluation crystallizability of macromolecules. InMethods in Enzymology, ed. RMS Charles, W Carter Jr, pp. 157–66. San Diego: Academic

10.1002/jcc.540090407

10.1038/343425a0

10.1038/80707

10.1016/S0079-6107(00)00009-2

10.1126/science.1925561

Higgins DG, 1992, Comput. Appl. Biosci., 8, 189

10.1038/80744

10.1006/jmbi.1993.1489

10.1006/jmbi.1993.1489

10.1073/pnas.31.6.153

10.1107/S0021889891004430

10.1016/S0959-440X(00)00100-7

10.1074/jbc.M100378200

10.1038/80763

10.1073/pnas.240454797

10.1107/S0021889892009944

10.1016/S0079-6107(00)00011-0

10.1110/ps.10101

10.1093/nar/30.1.264

10.1146/annurev.biophys.29.1.291

10.1016/S1367-5931(00)00221-0

10.1016/S0959-440X(00)00101-9

10.1038/80694

10.1006/mgme.2000.3073

10.1002/1615-9861(200201)2:1<11::AID-PROT11>3.3.CO;2-K

10.1016/S0969-2126(97)00260-8

10.1093/nar/27.1.275

10.1073/pnas.94.23.12366

10.1126/science.1063143

10.1002/prot.10118.abs

10.1093/nar/28.1.250

10.1073/pnas.95.23.13597

10.1042/0264-6021:3600001

10.1038/73723

10.1016/S0959-440X(00)00131-7

10.1016/S0959-440X(00)00215-3

10.1038/80700

Terwilliger TC, 2001, Acta Crystallogr., 57, 1755

10.1006/jmbi.2001.4513

Vaguine AA, 1999, Acta Crystallogr., 55, 191

10.1038/88640

Walsh MA, 1999, Acta Crystallogr., 55, 1168

10.1038/80712

10.1007/PL00000779

10.1016/S0969-2126(00)00533-5

Thông tin
Thông tin xuất bản

Annual Review of Genomics and Human Genetics

Tập 3 Số 1

243-262

Thông tin tác giả