Rules for optimization of biocatalysis in organic solvents

Biotechnology and Bioengineering - Tập 30 Số 1 - Trang 81-87 - 1987
Colja Laane1, Sjef Boeren1, Kees Vos1, C. Veeger1
1Department of Biochemistry, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands

Tóm tắt

AbstractGeneral rules for the optimization of different biocatalytic systems in various types of media containing organic solvents are derived by combining data from the literature, and the logarithm of the partition coefficient, log P, as a quantitative measure of solvent polarity. (1) Biocatalysis in organic solvents is low in polar solvents having a log P < 2, is moderate in solvents having a log P between 2 and 4, and is high in a polar solvents having a log P > 4. It was found that this correlation between polarity and activity parallels the ability of organic solvents to distort the essential water layer that stabilizes the biocatalysts. (2) Further optimization of biocatalysis in organic solvents is achieved when the polarity of the microenvironment of the biocatalyst (log Pi) and the continuous organic phase (log Pcph) is tuned to the polarities of both the substrate (log Ps) and the product (log Pp) according to the following rules: |log Pi − log Ps| and |log Pcph − log Pp| should be minimal and |log Pcph − log Ps| and |log Pi − log Pp| should be maximal, with the exception that in the case of substrate inhibition log Pi, should be optimized with respect to log Ps In addition to these simple optimization rules, the future developments of biocatalysis in organic solvents are discussed.

Từ khóa


Tài liệu tham khảo

10.1016/S0167-7799(84)80006-2

10.1073/pnas.82.10.3192

10.1002/bit.260270822

10.1016/0167-7799(85)90023-X

10.1002/bit.260250508

10.1016/S0021-9673(00)91610-8

Rekker R. F., 1979, Eur. J. Med. Chem. Chim. Therapeut., 14, 479

10.1111/j.1432-1033.1984.tb08488.x

Laane C., Recent Developments in Biotechnology

C.LaaneandC.Veeger Meth. Enz. (in press).

Laitinen H. A., 1975, Chemical Analysis, 361

Hildebrand J. H., 1970, Regular and Related Solutions

Crowley J. D., 1966, J. Paint Technol., 38, 269

10.1002/bit.260170802

10.1016/0003-9861(67)90197-X

Riddick J. A., 1970, Techniques of Chemistry, 1

Lange N. A., 1934, Handbook of Chemistry

M. H.Hilhorst Ph. D. Thesis Agricultural University Wageningen The Netherlands 1984.

1973, Handbook of Chemistry and physics.

Lewin S., 1974, Displacement of Water and its Control of Biochemical Reactions

10.1021/jo00338a045

10.1038/nbt0585-459

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Biotechnology and Bioengineering

Tập 30 Số 1

81-87

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