Role of the hematopoietic cytokines SCF, IL-3, GM-CSF and M-CSF in the diagnosis of pancreatic and ampullary cancer

International Journal of Biological Markers - Tập 27 Số 3 - Trang 186-194 - 2012
G. Vasiliades1, Nikolaos Kopanakis1, Michalis Vasiloglou1, George Zografos1, Helias Margaris1, Kyriaki Masselou2, Evniki Kokosi3, Theodore Liakakos4
1Third Surgical Department, General Hospital of Athens “G. Gennimatas”, Athens - Greece
2Department of Immunology, National Tissue Typing Center, General Hospital of Athens “G. Gennimatas”, Athens - Greece
3Department of Biochemistry, General Hospital of Athens “G. Gennimatas”, Athens - Greece
4Third Department of Surgery, Athens University Medical School, Attikon University Hospital, Athens - Greece

Tóm tắt

Background Previous studies have demonstrated altered levels of hematopoietic cytokines in the serum of patients with different types of cancer. Methods We measured the serum levels of the hematopoietic cytokines stem cell factor (SCF), interleukin 3 (IL-3), macrophage-colony stimulating factor (M-CSF) and granulocyte-macrophage-colony stimulating factor (GM-CSF) in 40 pancreatic and ampullary cancer patients and 40 healthy volunteers, using ELISA. We also assessed the most widely used pancreatic tumor markers, carbohydrate antigen 19–9 (CA 19–9) and carcinoembryonic antigen (CEA), in both groups. We then correlated the concentrations of the cytokines and the tumor markers in the patients’ serum and we estimated their diagnostic ability by calculating diagnostic sensitivity and specificity, positive and negative predictive values and the receiver operating characteristic (ROC) curve. Results The SCF and IL-3 levels were significantly lower and the M-CSF levels significantly higher in pancreatic cancer patients than in controls. There were significant positive correlations between the serum levels of CEA and M-CSF, GM-CSF and SCF, and between GM-CSF and IL-3. The area under the ROC curve and diagnostic sensitivity of M-CSF were greater than those of SCF and IL-3. The diagnostic sensitivity of the combined use of SCF and M-CSF reached 97.5%. Conclusion The diagnostic ability of M-CSF and SCF in pancreatic and ampullary cancer should stimulate further studies evaluating their clinical usefulness as tumor markers.

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Tài liệu tham khảo

10.3322/caac.20006

10.3322/caac.20073

Decker G.A., 2010, Gastroenterol Hepatol (NY), 6, 246

10.1016/j.ejso.2006.10.004

Steinberg W., 1990, Am J Gastroenterol, 85, 350

10.1097/00006676-199201000-00011

10.1093/annonc/mdp332

10.1001/archsurg.138.9.951

10.1038/bjc.1996.666

10.1093/clinchem/39.3.420

10.1038/bjc.1986.259

10.3748/wjg.v4.i2.147

Pasanen P.A., 1994, Br J Cancer, 69, 562, 10.1038/bjc.1994.102

10.1016/S0885-3924(00)00199-8

10.1054/blre.2001.0167

10.1515/CCLM.2004.253

Berdel W.E., 1992, Semin Oncol, 19, 41

10.1097/01.LAB.0000036875.21209.F9

10.1182/blood.V80.2.374.374

10.1002/jcp.1041430312

10.3109/08977199009003675

10.1038/sj.bjc.6690009

10.1159/000227443

Mc Dermott R.S., 2002, E Cytokine Network, 13, 121

Freelove R., 2006, Am Fam Physician, 73, 485

10.1093/annonc/10.suppl_4.S145

10.1007/s10620-005-2697-3

10.1515/CCLM.1999.141

10.1515/CCLM.2003.098

10.3109/00365519509090576

10.1016/j.cca.2007.02.037

10.1515/CCLM.2010.274

10.1515/CCLM.2004.047

10.1515/CCLM.2005.024

10.1515/CCLM.2007.025

Thông tin
Thông tin xuất bản

International Journal of Biological Markers

Tập 27 Số 3

186-194

Thông tin tác giả