Role of micronize purified flavonoid fraction and ethanol Graptophyllum pictum extract on experimental anal ulcer healing. Study on Wistar rat

Journal of Coloproctology - Tập 40 - Trang 105-111 - 2020
Sigit Adi Prasetyo1, Yan Wisnu1, Eriawan Agung Nugroho1, Edi Dharmana2, Neni Susilaningsih3, Ignatius Riwanto1
1Diponegoro University, Faculty of Medicine, Department of Surgery, Semarang, Indonesia
2Diponegoro University, Faculty of Medicine, Department of Parasitology and Immunology, Semarang, Indonesia
3Diponegoro University, Faculty of Medicine, Department of Histology, Semarang, Indonesia

Tóm tắt

Abstract Aim of the study To evaluate the role of micronized purified flavanoid fraction and ethanol Graptophyllum pictum extract in the treatment of anal ulcer. Method Twenty-eight Wistar rats were randomly allocated into four groups. Groups 2, 3 and 4 the anus were induced with croton oil, but was not induced on group 1. Groups 1 and 2 were treated with normal saline, while groups 3 and 4 were treated with micronized purified flavanoid fraction, and ethanol G. pictum extract, respectively. On 9th days blood sample were taken from the retro-orbital region, and Wistar was killed by cervical dislocation under ether anesthesia. The anal canal was resected up 2 cm from anal opening, weighted, photographically taken to measure the percentage of residual ulcer, and then prepared for microscopic examination. Elisa methods were done for superoxide dismutase and malondialdedhyde. The total leukocyte in the anal specimen was counted under 400 magnification power. superoxide dismutase, anal coefficient, and total leukocyte for statistical analysis were using ANOVA and LSD, while malondialdedhyde and percentage of ulcers were using Kruskal–Wallis and Mann–Whitney. Result Treatment with ethanol G. pictum extract dose of 100 mg/kg BW significantly reduces the percentage of anal ulcer, the edema, leukocyte infiltration, and malondialdedhyde, and increase the superoxide dismutase in comparison without treatment. Treatment with micronized purified flavanoid fraction did not reduce the leukocyte, anal coefficient, and percentage of anal ulcer, only increase malondialdedhyde and decrease superoxide dismutase significantly.

Tài liệu tham khảo

Gurel, 2013, Herbal haemorrhoidal cream for haemorrhoids, Chin J Physiol, 56, 253, 10.4077/CJP.2013.BAB127 AlAjmi, 2013, Some pharmacological actions of Myrica rubra part 1: effect on experimentally-induced gastric ulcers, inflammation and haemorrhoids in rats, African J Pharmacy Pharmacol, 7, 512, 10.5897/AJPP12.1204 Alonso-Coello, 2006, Meta-analysis of flavanoids for the treatment of haemorrhoids, Br J Surg, 93, 909, 10.1002/bjs.5378 Perera, 2012, Plebothropics for haemorrhoids, Cohrane Database Syst Rev, CD004322 ba-bai-ke-re, 2011, How we can improve patients’ comfort after Milligan–Morgan open haemor-rhoidectomy, World J Gastroenterol, 17, 1448, 10.3748/wjg.v17.i11.1448 Ozaki, 1989, Antiinflammatory effect of Graptophyllum pictum (L.) GRIFF, Chem Pharm Bull, 37, 2799, 10.1248/cpb.37.2799 Singh, 2015, Pharmacognostical evaluation of aerial parts of Graptophyllum pictum (L.) Griff (Syn: Justicia picta Linn.): a well-known folklore medicinal plant, Anc Sci Life, 34, 223, 10.4103/0257-7941.160868 Azeemuddin, 2014, An improved experimental model of hemorrhoids in rats: evaluation of antihemorrhoidal activity of an herbal formulation, ISRN Pharmacol, 2014, 1, 10.1155/2014/530931 Olagbende-Dada, 2009, A. Oxytocic and anti-implantation activities of the leaf extracts of Graptophyllum pictum (Linn.) Griff. (Acanthaceae), African J Biotechnol, 8, 5979, 10.5897/AJB09.565 Srinivasan, 2015, Nephroprotective potential of Graptophyllum pictum against renal injury induce by gentamycin, Iran J Basic Med Sci, 18, 412 Srinivasan, 2011, Effect of ethanol extract of Graptophyllum pictum (L.) Griff. on cisplatin induced nephrotoxicity in rats, Herba Polonica, 57, 51 Lyseng-Williamson, 2003, MIcronised purified flavanoid fraction. A review of its use in chronic insufficiency. Venous ulcers and haemorrhoids, Drugs, 63, 71, 10.2165/00003495-200363010-00005 Nair, 2016, A simple practice guide for dose conversion between animals and human, J Basic Clin Pharm, 7, 27, 10.4103/0976-0105.177703 Anonymous, 2011 Ashley, 2012, Inflammation: mechanisms, costs, and natural variation, Ann Rev Ecol Evol Syst, 43, 385, 10.1146/annurev-ecolsys-040212-092530 Sugimoto, 2016, Resolution of inflammation: what controls its onset?, Front Immunol, 7, 1, 10.3389/fimmu.2016.00160 Manson, 2012, Trauma alarmins as activators of damage-induced inflammation, Br J Surg, 99, 12, 10.1002/bjs.7717 Delano, 2016, The immune system's role in sepsis progression, resolution, and long-term outcome, Immunol Rev, 274, 330, 10.1111/imr.12499 Koh, 2011, Inflammation and wound healing: the role of the macrophage, Expert Rev Mol Med, 13, e23, 10.1017/S1462399411001943 Martinez, 2014, The M1 and M2 paradigm of macrophage activation: time for reassessment, F1000 Prime Rep, 6, 13, 10.12703/P6-13 Ayala, 2014, Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal, Oxid Med Cell Longev, 360438, 1, 10.1155/2014/360438 Fukai, 2011, Superoxide dismutases: role in redox signaling, vascular function, and diseases, Antioxid Redox Signal, 15, 1583, 10.1089/ars.2011.3999 Molteni, 2018, Natural products with toll-like receptor 4 antagonist activity, Int J Inflam, 1, 10.1155/2018/2859135 Katsenis, 2005, Micronized purified flavanoid fraction (MPFF): a review of its pharmacological effects, therapuetic efficacy and benefits in the management of chronic venous insufficiency, Curr Venous Pharmacol, 3, 1, 10.2174/1570161052773870 Singh, 2015, A phytopharmacological review on Justicia picta (Acanthaceae): a well known tropical folklore medicinal plant, J Coastal Life Med, 3, 1000, 10.12980/jclm.3.2015jclm-2015-0054
Thông tin
Thông tin xuất bản

Journal of Coloproctology

Tập 40

105-111

Thông tin tác giả