Role of endogenous platelet-activating factor in the regulation of pancreatic blood flow during caerulein stimulation
Tóm tắt
The role of endogenous platelet-activating factor (PAF) in the control of pancreatic blood flow during caerulein stimulation was investigated. Pancreatic blood flow in anesthetized rats was measured continuously by laser Doppler flowmetry for 2h during the intravenous infusion of caerulein (0.25 μg/kg per h). Pancreatic blood flow showed a gradual, consistent, and significant increase, reaching 114.2±2.3% of the basal value after 120 min. Changes in pancreatic blood flow induced by caerulein were completely inhibited by a cholecystokinin (CCK) antagonist (loxiglumide, 5 mg/kg per h, i.v.) and by a specific PAF antagonist (CV-6209, 1 mg/kg, i.v.-bolus). Systemic blood pressure remained stable in all groups. These results suggest an important role of endogenously yielded PAF in regulating pancreatic blood flow during caerulein stimulation to the pancreas.
Tài liệu tham khảo
Doi R, Hosotani R, Inoue K, et al. Effect of synthetic human cholecystokinin-33 on pancreatic blood flow in dogs. Pancreas 1990;5:615–620.
Homma T, Malik KU. Effect of secretin and caerulein in canine pancreas: Relation to prostaglandins. Am J Physiol 1983;244: G660–667.
Runkel N, Senninger N, Frank G, et al. Influence of caerulein on pancreatic blood flow (abstract). Digestion 1987;38(1):A55.
Polansky DB, Safaie-Shirazi S. Simultaneous measurement of pancreatic blood flow, endocrine, and exocrine secretion in conscious dogs. Surg Forum 1977;28:91–93.
Chung RS, Safaie-Shirazi S. The effect of secretin on pancreatic blood flow in the awake and anesthetized dog. Proc Soc Exp Biol Med 1983;173:620–625.
Soling HD, Fest W. Synthesis of 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor) in exocrine glands and its control by secretagogue. J Biol Chem 1986;261:13916–13922.
Soling HD, Eibl H, Fest W. Acetylcholine-like effects of 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (“platelet-activating factor”) and its analogues in exocrine secretory glands. Eur J Biochem 1984;144:65–72.
Terashita Z, Imura Y, Takatani M, et al. CV-6209, a highly potent antagonist of platelet-activating factor in vitro and in vivo. J Pharmacol Exp Ther 1987;242:263–268.
Terashita Z, Imura Y, Nishikawa K. Possible pathophysiological roles of platelet-activating factor in shock and the PAF antagonists, CV-3988 and CV-6209. In: Platelet-activating factor in endotoxin and immune diseases. Handley DA, Saunders RN, Houlihan WJ, Tomesch JC (eds) New York: Marcel Dekker, 1990;547–575.
Alder G, Bieger W, Kern HF. Amino acid transport in the rat exocrine pancreas. III. Effect of maximal and supramaximal hormonal stimulation in vivo. Cell Tiss Res 1978;194: 447–462.
Bieger W, Martin-Achard A, Bassler M, et al. Studies on intracellular transport of secretory proteins in the rat exocrine pancreas. IV. Stimulation by in vivo infusion of caerulein. Cell Tiss Res 1976;165:435–453.
Lampel M, Kern HF. Acute interstitial pancreatitis in the rat induced by excessive doses of a pancreatic secretagogue. Virchows Arch [A] 1977;373:97–117.
Norstein J, Lien B, Hall C, et al. Measurement of pancreas parenchyma perfusion by laser Doppler flowmetry. Transplant Proc 1990;22:607–608.
Maruyama Y, Atomi Y, Kimura W, et al. Sequential measurement of the pancreatic islet or non-islet blood flow, stimulated by either caerulein and glucose (abstract). Gastroenterology 1993;104:A321.
Leung FW. Comparison of blood flow measurement by hydrogen gas clearance and laser Doppler flowmetry in the rat duodenum. Scand J Gastroenterol 1990;25:429–434.
Yamaguchi T, Ogura M, Kubota Y, Fujimura K, Binnaka T, Tani K, Kitagawa S, Mizuno T, Inoue K. Continuous monitoring of pancreatic microcirculation in rats by laser Doppler flowmetry and reflectance spectrophotometry. In: Tsuchiya M, Asano M, Katori M (eds) Microcirculation annal 1991. Tokyo: Nihon-Igakukan, 1991;91–92.
Jackson CV, Schumacher WA, Kunkel SL, et al. Platelet-activating factor and the release of a platelet-derived coronary artery vasodilator substance in the canine. Circ Res 1986;58:218–229.
Chu K, Gerber JG, Nies AS. Local vasodilator effect of platelet-activating factor in the gastric, mesentric and femoral arteries of the dog. J Pharmacol Exp Ther 1988;246:996–1000.
Binnaka T, Yamaguchi T, Kubota Y, et al. Gastric hemodynamic disturbance induced by hemorrhagic shock in rats: Role of platelet-activating factor. Scand J Gastroenterol 1990;25:555–562.