Role of Ppt1 in multiple stress responses in Candida albicans

Science China Press., Co. Ltd. - Tập 59 - Trang 4060-4068 - 2014
Kangdi Hu1,2, Wanjie Li1, Jiaxin Gao1, Qizheng Liu1, Haitao Wang1,3, Yue Wang3, Jianli Sang1
1Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education, College of Life Science, Beijing Normal University, Beijing, China
2School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, China
3Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore

Tóm tắt

To study the function of CaPpt1, we deleted PPT1 gene from the Candida albicans genome by sequentially replacing the entire coding region with the selectable markers ARG4 and HIS1. The results showed that the deletion of Ppt1 did not affect the hyphal formation of C. albicans under serum induction and caused enhanced sensitivity to DNA damage, Calcofluor white and salt-induced stress. We also found that Ppt1 was not required for the phenotypic response of cells treated with the genotoxins, methylmethane sulfonate and hydroxyurea. Flow cytometric analyses indicated that ppt1Δ cells and wild-type cells showed similar G2/M arrest profiles when exposed to DNA damage stress. Ppt1 was not required for the activation of the DNA damage response pathway, as indicated by normal phosphorylation of Rad53 and Rfa2 in ppt1Δ cells under DNA damage stress. We suggest that Ppt1 plays important roles in response to various stress conditions in C. albicans.

Tài liệu tham khảo

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