Role of Heat Shock Transcription Factor in Saccharomyces cerevisiae Oxidative Stress Response

American Society for Microbiology - Tập 6 Số 8 - Trang 1373-1379 - 2007
Ayako Yamamoto1, Junko Ueda1, Noritaka Yamamoto1, Naoya Hashikawa1, Hiroshi Sakurai1
1Division of Health Sciences, Graduate School of Medical Science, Kanazawa University, 5-11-80 Kodatsuno, Kanazawa, Ishikawa 920-0942, Japan

Tóm tắt

ABSTRACT The heat shock transcription factor Hsf1 of the yeast Saccharomyces cerevisiae regulates the transcription of a set of genes that contain heat shock elements (HSEs) in their promoters and function in diverse cellular processes, including protein folding. Here, we show that Hsf1 activates the transcription of various target genes when cells are treated with oxidizing reagents, including the superoxide anion generators menadione and KO 2 and the thiol oxidants diamide and 1-chloro-2,4-dinitrobenzene (CDNB). Similar to heat shock, the oxidizing reagents are potent inducers of both efficient HSE binding and extensive phosphorylation of Hsf1. The inducible phosphorylation of Hsf1 is regulated by the intramolecular domain-domain interactions and affects HSE structure-specific transcription. Unlike the heat shock, diamide, or CDNB response, menadione or KO 2 activation of Hsf1 is inhibited by cyclic-AMP-dependent protein kinase (PKA) activity, which negatively regulates the activator functions of other transcriptional regulators implicated in the oxidative stress response. These results demonstrate that Hsf1 is a member of the oxidative stress-responsive activators and that PKA is a general negative regulator in the superoxide anion response.

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Tài liệu tham khảo

10.1101/gad.1044503

10.1128/EC.2.2.351-361.2003

10.1002/(SICI)1097-0061(19980915)14:12<1127::AID-YEA300>3.0.CO;2-#

10.1091/mbc.12.2.323

10.1016/j.molcel.2004.05.021

10.1007/s004380050017

10.1128/EC.2.5.1018-1024.2003

10.1534/genetics.104.034256

10.1128/MCB.17.12.6982

10.1099/00221287-139-3-501

10.1091/mbc.11.12.4241

10.1101/gad.12.4.586

10.1128/MCB.24.12.5249-5256.2004

10.1046/j.1365-2958.2002.03011.x

10.1128/MCB.24.9.3648-3659.2004

10.1074/jbc.M510827200

10.1074/jbc.M609708200

10.1128/EC.4.6.1050-1056.2005

10.1016/0014-5793(95)00603-7

10.1128/jb.174.20.6678-6681.1992

10.1007/BF02208613

10.1074/jbc.274.23.16040

10.1091/mbc.11.5.1753

10.1101/gad.10.5.592

10.1002/j.1460-2075.1996.tb00576.x

10.1093/emboj/16.5.1035

10.1128/EC.2.3.381-389.2003

10.1096/fj00-0294rev

10.1091/mbc.11.7.2335

10.1073/pnas.93.12.5777

10.1074/jbc.M200559200

10.1093/emboj/17.13.3556

10.1016/S0092-8674(88)91219-6

Takemori, Y., A. Sakaguchi, S. Matsuda, Y. Mizukami, and H. Sakurai. 2005. Stress-induced transcription of the endoplasmic reticulum oxidoreductin gene ERO1 in the yeast Saccharomyces cerevisiae. Mol. Genet. Genomics275:89-96.

10.1016/j.tcb.2005.04.003

10.1046/j.1365-2958.1999.01538.x

10.1016/S0021-9258(18)33730-X

10.1073/pnas.0305888101

Trott, A., and K. A. Morano. 2003. The yeast response to heat shock, p. 71-119. In S. Hohmann and P. W. H. Mager (ed.), Yeast stress responses. Springer-Verlag, Heidelberg, Germany.

10.1379/CSC-14R.1

10.1074/jbc.M411256200

10.1093/emboj/cdf528

Zadzinski, R., A. Fortuniak, T. Bilinski, M. Grey, and G. Bartosz. 1998. Menadione toxicity in Saccharomyces cerevisiae cells: activation by conjugation with glutathione. Biochem. Mol. Biol. Int.44:747-759.

10.1016/S1097-2765(00)80118-5

10.1379/1466-1268(1998)003<0130:CBGOAT>2.3.CO;2

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American Society for Microbiology

Tập 6 Số 8

1373-1379

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