Role of H+-ATPase and Alternative Oxidase in Regulation of Intracellular pH at Different Stages of Development of the Tobacco Male Gametophyte
Tóm tắt
In order to determine the role of the plasma membrane H+-ATPase and alternative oxidase (alternative pathway of respiration) in the regulation of intracellular pH during development of the tobacco male gametophyte, we studied the changes in pH due to the inhibition of these enzymes by orthovadanate and benzhydroxamic acid, respectively. The inhibition of these enzymes decreased the intracellular pH at all three studied stages of the male gametophyte development: middle and late binuclear pollen grains and activated mature pollen grain. The data obtained suggest that H+-ATPase and alternative oxidase are involved in the regulation of intracellular pH of the pollen grain during its differentiation and activation that precede germination. At the same time, during the recovery of intracellular pH after its acidification by propionic acid, it was found that other mechanisms, not related to the above mentioned, greatly contribute to the regulation of pH.
Tài liệu tham khảo
Andreyuk, D.S., Matveeva, N.P., Tukeeva, M.I., et al., Dynamics of Inorganic Ions in the Tobacco Microspore and Pollen Grain during Male Gametophyte Development, Ontogenez (Moscow), 2000, vol. 31, no. 2, pp. 114-119.
Andreyuk, D.S., Matveeva, N.P., Tukeeva, M.I., et al., Intracellular Concentrations of Potassium, Chloride, and Protons during Differentiation of the Tobacco Male Gametophyte, Fiziol. Rast. (Moscow), 2001, vol. 48, no. 3, pp. 455-460.
Andreyuk, D.S., Voyscech, O.O., Matveyeva, N.P., et al., Changes in Intracellular pH during Tobacco Pollen Development and Germination, Plant Physiol. Biochem. (Paris), 2000, vol. 38, suppl., p. S49.
Day, D.A.D., Krab, K., Lambers, H., et al., The Cyanide-Resistant Oxidase: To Inhibit or Not to Inhibit, That Is the Question, Plant Physiol., 1996, vol. 110, no. 1, pp. 1-2.
Feijo, J.A., Malho, R., and Obermeyer, G., Ion Dynamics and Possible Role during in vitro Pollen Germination and Tube Growth, Protoplasma, 1995, vol. 187, nos. 1-4, pp. 155-167.
Fricker, M.D., White, N.S., and Obermeyer, G., pH Gradients Are Not Associated with Tip Growth in Pollen Tubes of Lilium longiflorum, J. Cell Sci., 1997, vol. 110, part 15, pp.1729-1740.
Haugland, P., Handbook of Fluorescent Probes and Research Chemicals, Leiden: Molecular Probes Inc., 1996.
Heslop-Harrison, J., Pollen Germination and Pollen Tube Growth, Int. Rev. Cytol., 1987, vol. 107, pp. 1-78.
Hoekstra, F.A. and Bruinsma, J., Control of Respiration of Binucleate and Trinucleate Pollen Under Humid Conditions, Physiol. Plant., 1980, vol. 48, no. 1, pp. 71-77.
Kurkdjian, A. and Guern, J., Intracellular pH: Measurement and Importance in Cell Activity, Annu. Rev. Plant Physiol. Plant Mol. Biol. (Palo Alto), 1989, vol. 40, pp. 271-303.
Lambers, H., Respiration in Intact Plant and Tissues: Its Regulation and Dependence on Environmental Factors, Metabolism and Invaded Organisms, Higher Plant Cell Respiration, Berlin: Springer, 1985, pp. 173-201.
Mathieu, Y., Guern, J., and Pean, M., Cytoplasmic pH Regulation in Acer pseudoplatanus Cells. 2. Possible Mechanisms Involved in pH Regulation during Acid-Load, Plant Physiol., 1986, vol. 82, no. 3, pp. 846-852.
Nitsch, J.P., Deux Espéces Photoperiodiques de Jours Courts: Plumbago indica L. et P. zeylanica L., Bull. Soc. Bot.Fr., 1965, vol. 112, no. 9, pp. 517-522.
Obermeyer, G., Lützelschwab, M., Heumann, H.G., et al., Immunolocalization of H+-ATPases in the Plasma Membrane of Pollen Grains and Pollen Tubes of Lillum longiflorum, Protoplasma, 1992, vol. 171, pp. 55-63.
Obermeyer, G., Kriechbaumer, P., Strasser, D., et al., Boric Acid Stimulates the Plasma Membrane H+-ATPase of Ungerminated Lily Pollen Grains, Physiol. Plant., 1996, vol. 98, no. 2, pp. 281-290.
Reznikova, S.A., Tsitologiya i fiziologiya razvivayushchegosya pyl'nika (Cytology and Physiology of the Developing Anther), Moscow: Nauka, 1984.
Sakano, K., Revision of Biochemical pH-Stat: Involvement of Alternative Pathway Metabolisms, Plant Cell Physiol., 1998, vol. 39, no. 5, pp. 467-473.
Scarborough, G.A., Structure and Function of the P-Type ATPase, Curr. Opin. Cell Biol., 1999, vol. 11, pp. 517-522.
Schrauwen, J.A.M., de Groot, P.F.M., van Herpen, M.M.A., et al., Stage-Related Expression of mRNAs during Pollen Development in Lily and Tobacco, Planta, 1990, vol. 182, no. 2, pp. 298-304.
Serrano, R., Structure and Function of Plasma Membrane ATPase, Annu. Rev. Plant Physiol. Plant Mol. Biol. (Palo Alto), 1989, vol. 40, pp. 61-94.
Sze, H., Li, X., and Palmgren, M.G., Energization of Plant Cell Membranes by H+-Pumping ATPases: Regulation and Biosynthesis, Plant Cell, 1999, vol. 11, no. 4, pp. 677-689.
Taylor, L. and Hepler, P., Pollen Germination and Tube Growth, Annu. Rev. Plant Physiol. Plant Mol. Biol. (Palo Alto), 1997, vol. 48, pp. 461-491.
Trofimova, M.S., H+-ATPase of Plasmalemma as a Component of pH-Stat of the Cytosol of Isolated Protoplasts, Fiziol. Rast. (Moscow), 1992, vol. 39, no. 1, pp. 5-14.
Tukeeva, M.I., Matveeva, N.P., and Ermakov, I.P., Respiration of Microspores during Induction of Pollen Embryogenesis in the Tobacco, Fiziol. Rast., 1994, vol. 41, no. 6, pp.821-825.
Tukeeva, M.I., Matveeva, N.P., Degasyuk, A.P., et al., Changes in Pathways of Respiration during Formation of the Tobacco Pollen Grain, Fiziol. Rast., 1998, vol. 45, no. 1, pp.43-47.
Tupy, J., Rihova, L., Capkova, V., et al., Differentiation and Maturation of Tobacco Pollen in situ and in Suspension Culture, Angiosperm Pollen and Ovules, New York: Springer, 1992, pp. 309-314.
Vanlerberghe, G.C. and McIntosh, L., Alternative Oxidase: from Gene to Function, Annu. Rev. Plant Physiol., Plant Mol. Biol. (Palo Alto), 1997, vol. 48, pp. 703-734.
Vasil, I.K., Physiology and Culture of Pollen, Int. Rev. Cytol., 1987, vol. 107, pp. 127-174.