Rinderpest Virus RNA Polymerase Subunits: Mapping of Mutual Interacting Domains on the Large Protein L and Phosphoprotein P
Tóm tắt
The RNA dependant RNA polymerase of negative sense RNA viruses is composed of two subunits – the Large protein (L) and the Phosphoprotein (P). These two proteins have to form a complex in order to carry out genome transcription and replication. Employing the baculovirus expression system, we demonstrate here, the specific in vivo interaction between the L and P proteins of Rinderpest virus and also the stabilization of L protein when it is present as L + P complex. The regions on either protein involved in such interaction has been studied using the yeast two-hybrid system which indicates that the P binding region resides within the amino terminal 380 amino acid residues of L protein. The L binding region on P protein has been mapped to lie within 347–490 amino acids.
Tài liệu tham khảo
Barrett T., Subbarao S.M., Belsham, G.J., and Mahy, B.W., in Kingsbury D.W. (ed.), The Paramyxoviruses. Plenum Press, New York, 1991, pp. 83–102.
Baron M.D., Shaila M.S., and Barrett T., J. Gen. Virol 74, 299–304, 1993.
Baron M.D. and Barrett T., J Gen Virol 76, 593–602, 1995.
Galinski M.S., Adv Vir Res 39, 129–162, 1991.
Hunt D.M., Smith E.F., and Buckley D.W., J Virol 52, 515–421, 1984.
De B.P. and Banerjee A.K., Biochem Biophys Res Commun 126, 40–49, 1985.
Hercyk N., Horikami S.M., and Moyer S.A., Virology 163, 222–225, 1988.
Einberger H., Mertz R., Hofschneider P.H., and Neubert W.J., J Virol 64, 4274–4280,1990.
Hammond D.C., Haily B.E., and Lesnaw J.A., J Gen Virol 73, 67–75, 1992.
Barik S. and Banerjee A.K., J Virol 66, 1109–1118,1992a.
Horikami S.M., Smallwood S., Bankamp B., and Moyer S.A., Virology 205, 540–545, 1994.
Smallwood S., Ryan K.W., and Moyer S.A., Virology 202, 154–163,1994.
Canter D.M. and Perrault J., Virology 279, 376–386, 1996.
Chandrika R., Horikami S.M., Smallwood S., and Moyer S.A., Virology 273, 352–363,1995.
Holmes D.E. and Moyer S.A., J Virol 76, 3078–3083, 2002.
Parks, G.D., J Virol 68, 4862–4872, 1994.
Horikami S.M., Curran J., Kolakofsky D., and Moyer S.A., J Virol 66, 4901–4908, 1992.
Emerson S.U. and Schubert M., Proc Natl Acad Sci, USA 84, 5655–5659, 1987.
Raha T., Chattopadhyay A., and Shaila M.S., Virus Res., 99(2), 131–138, 2004.
Shaji D. and Shaila M.S., Virology 258, 415–424, 1999.
Takas A.M. and Banerjee A.K., Virology 208, 821–826, 1995.
Chenik M., Schnell M., Conzelmann K.K., and Blondel D., J Virol 72, 1925–1930, 1998.
Barik S. and Banerjee A.K., Proc Natl Acad Sci, USA 89, 6570–6574, 1992b.