Mã vàng Ribonuclease Gắn Kết Chondroitin Sulphate Trong Granule Basophil Của Chuột Lang

Springer Science and Business Media LLC - Tập 30 - Trang 603-608 - 1998
Ann M. Dvorak, Ellen S. Morgan

Tóm tắt

Các bạch cầu ưa base là những tế bào granulocyte tiết có chứa hạt metachromatic, chứa một hỗn hợp các proteoglycan hạt thiếu heparin. Trong chuột lang, các hạt bạch cầu ưa base được tách biệt chủ yếu chứa chondroitin sulphate. Gần đây, chúng tôi đã chứng minh rằng một kỹ thuật gắn enzyme-vàng để hình ảnh RNA, sử dụng thuốc thử RNase vàng, cũng liên kết cụ thể với heparin trong các hạt tế bào mast của người. Sự liên kết này dựa trên đặc tính đã biết của heparin như một chất ức chế cạnh tranh của RNase. Sử dụng các phương pháp tương tự, chúng tôi cho thấy RNase-vàng gắn kết với chondroitin sulphate trong các hạt tiết của bạch cầu ưa base chuột lang, từ đó mở rộng khả năng áp dụng của phương pháp gắn vàng sau khi nhúng này cho các nghiên cứu yêu cầu hình ảnh hóa chondroitin sulphate trong các mẫu chuẩn bị điện tử thường xuyên.

Từ khóa

#bạch cầu ưa base #chondroitin sulphate #RNase vàng #chuột lang #hình ảnh hóa điện tử

Tài liệu tham khảo

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