Rhizobium sophorae sp. nov. and Rhizobium sophoriradicis sp. nov., nitrogen-fixing rhizobial symbionts of the medicinal legume Sophora flavescens

International Journal of Systematic and Evolutionary Microbiology - Tập 65 Số Pt_2 - Trang 497-503 - 2015
Yin Jiao1,2,3, Hui Yan1,2,3, Zhao Ji1,2,3, Yuan Hui Liu1,2,3, Xin Hua Sui1,2,3, En Tao Wang1,4,2,3, Bao Lin Guo5, Wen Xin Chen1,2,3, Wen Feng Chen1,2,3
1College of Biological Sciences and Rhizobia Research Center, China Agricultural University, Beijing 100193, PR China
2MOA Key Laboratory of Soil Microbiology, Beijing 100193, PR China
3State Key Laboratory of Agrobiotechnology, Beijing 100193, PR China
4Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México D.F. 11340, México
5Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, PR China

Tóm tắt

Five bacterial strains representing 45 isolates originated from root nodules of the medicinal legume Sophora flavescens were defined as two novel groups in the genus Rhizobium based on their phylogenetic relationships estimated from 16S rRNA genes and the housekeeping genes recA, glnII and atpD. These groups were distantly related to Rhizobium leguminosarum USDA 2370T (95.6 % similarity for group I) and Rhizobium phaseoli ATCC 14482T (93.4 % similarity for group II) in multilocus sequence analysis. In DNA–DNA hybridization experiments, the reference strains CCBAU 03386T (group I) and CCBAU 03470T (group II) showed levels of relatedness of 17.9–57.8 and 11.0–42.9 %, respectively, with the type strains of related species. Both strains CCBAU 03386T and CCBAU 03470T contained ubiquinone 10 (Q-10) as the major respiratory quinone and possessed 16 : 0, 18 : 0, 19 : 0 cyclo ω8c, summed feature 8 and summed feature 2 as major fatty acids, but did not contain 20 : 3 ω6,8,12c. Phenotypic features distinguishing both groups from all closely related species of the genus Rhizobium were found. Therefore, two novel species, Rhizobium sophorae sp. nov. for group I (type strain CCBAU 03386T = E5T = LMG 27901T = HAMBI 3615T) and Rhizobium sophoriradicis sp. nov. for group II (type strain CCBAU 03470T = C-5-1T = LMG 27898T = HAMBI 3510T), are proposed. Both groups were able to nodulate Phaseolus vulgaris and their hosts of origin (Sophora flavescens) effectively and their nodulation gene nodC was phylogenetically located in the symbiovar phaseoli.

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International Journal of Systematic and Evolutionary Microbiology

Tập 65 Số Pt_2

497-503

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