Rheological Properties of Cross‐Linked Hyaluronan–Gelatin Hydrogels for Tissue Engineering

Macromolecular Bioscience - Tập 9 Số 1 - Trang 20-28 - 2009
Janssen L. Vanderhooft1, Mataz Alcoutlabi2, Jules J. Magda3,2, Glenn D. Prestwich4,5
1Department of Bioengineering, University of Utah, 419 Wakara Way, Suite 205, Salt Lake City, Utah 84108-1257, USA
2Department of Materials Science and Engineering, University of Utah, 122 South Central Campus Drive, Room 304, Salt Lake City, Utah 84108-1257, USA
3Department of Chemical Engineering, University of Utah, 50 South Central Campus Drive, Room 3290, Salt Lake City, Utah 84108-1257, USA
4Center for Therapeutic Biomaterials, University of Utah, 419 Wakara Way, Suite 205, Salt Lake City, Utah 84108-1257, USA
5Department of Medicinal Chemistry, University of Utah, 419 Wakara Way, Suite 205, Salt Lake City, Utah 84108-1257, USA

Tóm tắt

Abstract

Hydrogels that mimic the natural extracellular matrix (ECM) are used in three‐dimensional cell culture, cell therapy, and tissue engineering. A semi‐synthetic ECM based on cross‐linked hyaluronana offers experimental control of both composition and gel stiffness. The mechanical properties of the ECM in part determine the ultimate cell phenotype. We now describe a rheological study of synthetic ECM hydrogels with storage shear moduli that span three orders of magnitude, from 11 to 3 500 Pa, a range important for engineering of soft tissues. The concentration of the chemically modified HA and the cross‐linking density were the main determinants of gel stiffness. Increase in the ratio of thiol‐modified gelatin reduced gel stiffness by diluting the effective concentration of the HA component.

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Thông tin xuất bản

Macromolecular Bioscience

Tập 9 Số 1

20-28

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