Revisiting the white blood cell count: immature granulocytes count as a diagnostic marker to discriminate between SIRS and sepsis - a prospective, observational study

Axel Nierhaus1, Stefanie Klatte2, Jo Linssen2, Nina Marie Eismann1, Dominic Wichmann1, Jörg Hedke1, Stephan Braune1, Stefan Kluge1
1Department of Critical Care, University Medical Center Hamburg-Eppendorf, Martinistr. 52, Hamburg, 20246, Germany
2Sysmex Europe GmbH, Bornbarch 1, Norderstedt, 22848, Germany

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Bone RC, Balk RA, Cerra FB, Dellinger RP, Fein AM, Knaus WA, Schein RM, Sibbald WJ: Definitions for sepsis and organ failure and guidelines for the use of innovative therapies in sepsis. The ACCP/SCCM consensus conference committee. American college of chest physicians/society of critical care medicine. Chest. 1992, 101 (6): 1644-1655. 10.1378/chest.101.6.1644.

Levy MM, Fink MP, Marshall JC, Abraham E, Angus D, Cook D, Cohen J, Opal SM, Vincent JL, Ramsay G: SCCM/ESICM/ACCP/ATS/SIS international sepsis definitions conference. Intensive Care Med. 2001, 29 (4): 530-538.

Schoenberg MH, Weiss M, Radermacher P: Outcome of patients with sepsis and septic shock after ICU treatment. Langenbecks Arch Surg. 1998, 383 (1): 44-48. 10.1007/s004230050090.

Martin GS, Mannino DM, Eaton S, Moss M: The epidemiology of sepsis in the United States from 1979 through 2000. N Engl J Med. 2003, 348 (16): 1546-1554. 10.1056/NEJMoa022139.

Rivers E, Nguyen B, Havstad S, Ressler J, Muzzin A, Knoblich B, Peterson E, Tomlanovich M: Early goal-directed therapy in the treatment of severe sepsis and septic shock. N Engl J Med. 2001, 345 (19): 1368-1377. 10.1056/NEJMoa010307.

Rangel-Frausto MS, Pittet D, Costigan M, Hwang T, Davis CS, Wenzel RP: The natural history of the systemic inflammatory response syndrome (SIRS). A prospective study. JAMA. 1995, 273 (2): 117-123. 10.1001/jama.1995.03520260039030.

Castelli GP, Pognani C, Cita M, Stuani A, Sgarbi L, Paladini R: Procalcitonin, C-reactive protein, white blood cells and SOFA score in ICU: diagnosis and monitoring of sepsis. Minerva Anestesiol. 2006, 72 (1–2): 69-80.

Iapichino G, Marzorati S, Umbrello M, Baccalini R, Barassi A, Cainarca M, Colombo Pavini F, Mantovani E, Mauri A, Moroni B, Noto A, Melzi D’eril GV, Langer M: Daily monitoring of biomarkers of sepsis in complicated long-term ICU-patients: can it support treatment decisions?. Minerva Anestesiol. 2010, 76 (10): 814-23.

Buttenschoen K, Buttenschoen DC, Berger D, Vasilescu C, Schafheutle S, Goeltenboth B, Seidelmann M, Beger HG: Endotoxemia and acute-phase proteins in major abdominal surgery. Am J Surg. 2001, 181 (1): 36-43. 10.1016/S0002-9610(00)00534-1.

Shin HS, Xu F, Bagchi A, Herrup E, Prakash A, Valentine C, Kulkarni H, Wilhelmsen K, Warren S, Hellman J: Bacterial lipoprotein TLR2 agonists broadly modulate endothelial function and coagulation pathways in vitro and in vivo. J Immunol. 2011, 186 (2): 1119-1130. 10.4049/jimmunol.1001647.

Martin C, Boisson C, Haccoun M, Thomachot L, Mege JL: Patterns of cytokine evolution (tumor necrosis factor-alpha and interleukin-6) after septic shock, hemorrhagic shock, and severe trauma. Crit Care Med. 1997, 25 (11): 1813-1819. 10.1097/00003246-199711000-00018.

Oberhoffer M, Vogelsang H, Russwurm S, Hartung T, Reinhart K: Outcome prediction by traditional and new markers of inflammation in patients with sepsis. Clin Chem Lab Med. 1999, 37 (3): 363-368.

Engler R: Acute-phase proteins in inflammation. C R Seances Soc Biol Fil. 1995, 189 (4): 563-578.

Grube BJ, Cochane CG, Ye RD, Green CE, McPhail ME, Ulevitch RJ, Tobias PS: Lipopolysaccharide binding protein expression in primary human hepatocytes and HepG2 hepatoma cells. J Biol Chem. 1994, 269 (11): 8477-8482.

Meisner M, Tschaikowsky K, Hutzler A, Schick C, Schuttler J: Postoperative plasma concentrations of procalcitonin after different types of surgery. Intensive Care Med. 1998, 24 (7): 680-684. 10.1007/s001340050644.

Brunkhorst FM, Clark AL, Forycki ZF, Anker SD: Pyrexia, procalcitonin, immune activation and survival in cardiogenic shock: the potential importance of bacterial translocation. Int J Cardiol. 1999, 72 (1): 3-10. 10.1016/S0167-5273(99)00118-7.

Castelli GP, Pognani C, Cita M, Paladini R: Procalcitonin as a prognostic and diagnostic tool for septic complications after major trauma. Crit Care Med. 2009, 37 (6): 1845-1849. 10.1097/CCM.0b013e31819ffd5b.

Schumann RR, Leong SR, Flaggs GW, Gray PW, Wright SD, Mathison JC, Tobias PS, Ulevitch RJ: Structure and function of lipopolysaccharide binding protein. Science. 1990, 249 (4975): 1429-1431. 10.1126/science.2402637.

Gaini S, Koldkjaer OG, Moller HJ, Pedersen C, Pedersen SS: A comparison of high-mobility group-box 1 protein, lipopolysaccharide-binding protein and procalcitonin in severe community-acquired infections and bacteraemia: a prospective study. Crit Care. 2007, 11 (4): R76-10.1186/cc5967.

Prucha M, Herold I, Zazula R, Dubska L, Dostal M, Hildebrand T, Hyanek J: Significance of lipopolysaccharide-binding protein (an acute phase protein) in monitoring critically ill patients. Crit Care. 2003, 7 (6): R154-R159. 10.1186/cc2386.

Adamzik M, Eggmann M, Frey UH, Gorlinger K, Brocker-Preuss M, Marggraf G, Saner F, Eggebrecht H, Peters J, Hartmann M: Comparison of thromboelastometry with procalcitonin, interleukin 6, and C-reactive protein as diagnostic tests for severe sepsis in critically ill adults. Crit Care. 2010, 14 (5): R178-10.1186/cc9284.

Weiland T, Kalkman H, Heihn H: Evaluation of the automated immature granulocyte count (IG) on Sysmex XE-2100 automated haematology analyser vs. visual microscopy (NCCLS H20-A). Sysmex J Int. 2002, 12 (2): 63-70.

Ansari-Lari MA, Kickler TS, Borowitz MJ: Immature granulocyte measurement using the Sysmex XE-2100. Relationship to infection and sepsis. Am J Clin Pathol. 2003, 120 (5): 795-799. 10.1309/LT30BV9UJJV9CFHQ.

Nigro KG, O’Riordan M, Molloy EJ, Walsh MC, Sandhaus LM: Performance of an automated immature granulocyte count as a predictor of neonatal sepsis. Am J Clin Pathol. 2005, 123 (4): 618-624. 10.1309/73H7K7UBW816PBJJ.

Bender L, Thaarup J, Varming K, Krarup H, Ellermann-Eriksen S, Ebbesen F: Early and late markers for the detection of early-onset neonatal sepsis. Dan Med Bull. 2008, 55 (4): 219-223.

Smith JA: Neutrophils, host defense, and inflammation: a double-edged sword. J Leukoc Biol. 1994, 56 (6): 672-686.

Panopoulos AD, Watowich SS: Granulocyte colony-stimulating factor: molecular mechanisms of action during steady state and ‘emergency’ hematopoiesis. Cytokine. 2008, 42 (3): 277-288. 10.1016/j.cyto.2008.03.002.

Selig C, Nothdurft W: Cytokines and progenitor cells of granulocytopoiesis in peripheral blood of patients with bacterial infections. Infect Immun. 1995, 63 (1): 104-109.

Thijs LG, Hack CE: Time course of cytokine levels in sepsis. Intensive Care Med. 1995, 21 (Suppl 2): S258-S263.

Glas AS, Lijmer JG, Prins MH, Bonsel GJ, Bossuyt PM: The diagnostic odds ratio: a single indicator of test performance. J Clin Epidemiol. 2003, 56 (11): 1129-1135. 10.1016/S0895-4356(03)00177-X.

Vincent JL: Organ dysfunction in patients with severe sepsis. Surg Infect (Larchmt). 2006, 7 (Suppl 2): S69-S72.

Vosylius S, Sipylaite J, Ivaskevicius J: Sequential organ failure assessment score as the determinant of outcome for patients with severe sepsis. Croat Med J. 2004, 45 (6): 715-720.

Bozza FA, Salluh JI, Japiassu AM, Soares M, Assis EF, Gomes RN, Bozza MT, Castro-Faria-Neto HC, Bozza PT: Cytokine profiles as markers of disease severity in sepsis: a multiplex analysis. Crit Care. 2007, 11 (2): R49-10.1186/cc5783.

Kellum JA, Kong L, Fink MP, Weissfeld LA, Yealy DM, Pinsky MR, Fine J, Krichevsky A, Delude RL, Angus DC: Understanding the inflammatory cytokine response in pneumonia and sepsis: results of the genetic and inflammatory markers of sepsis (GenIMS) study. Arch Intern Med. 2007, 167 (15): 1655-1663. 10.1001/archinte.167.15.1655.

Silvestre J, Povoa P, Coelho L, Almeida E, Moreira P, Fernandes A, Mealha R, Sabino H: Is C-reactive protein a good prognostic marker in septic patients?. Intensive Care Med. 2009, 35 (5): 909-913. 10.1007/s00134-009-1402-y.

Villar J, Perez-Mendez L, Espinosa E, Flores C, Blanco J, Muriel A, Basaldua S, Muros M, Blanch L, Artigas A, Kacmarek RM: Serum lipopolysaccharide binding protein levels predict severity of lung injury and mortality in patients with severe sepsis. PLoS One. 2009, 4 (8): e6818-10.1371/journal.pone.0006818.

Liaudat S, Dayer E, Praz G, Bille J, Troillet N: Usefulness of procalcitonin serum level for the diagnosis of bacteremia. Eur J Clin Microbiol Infect Dis. 2001, 20 (8): 524-527. 10.1007/s100960100548.

National Committee for Clinical Laboratory Standards (NCCLS): Reference leukocyte differential count (proportional) and evaluation of instrumental methods. 1992, Wayne, PA: NCCLS Document H20-A 1

Bruegel M, Fiedler GM, Matthes G, Thiery J: Reference values for immature granulocytes in healthy blood donors generated on the Sysmex XE-2100 automated hematology analyser. Sysmex J Int. 2004, 14 (1): 5-7.

Fernandes B, Hamaguchi Y: Automated enumeration of immature granulocytes. Am J Clin Pathol. 2007, 128 (3): 454-463. 10.1309/TVGKD5TVB7W9HHC7.

Mardi D, Fwity B, Lobmann R, Ambrosch A: Mean cell volume of neutrophils and monocytes compared with C-reactive protein, interleukin-6 and white blood cell count for prediction of sepsis and nonsystemic bacterial infections. Int J Lab Hematol. 2010, 32 (4): 410-418.

Le Gall JR, Lemeshow S, Saulnier F: A new simplified acute physiology score (SAPS II) based on a european/north american multicenter study. JAMA. 1993, 270 (24): 2957-2963. 10.1001/jama.1993.03510240069035.

Vincent JL, Moreno R, Takala J, Willatts S, De Mendonca A, Bruining H, Reinhart CK, Suter PM, Thijs LG: The SOFA (sepsis-related organ failure assessment) score to describe organ dysfunction/failure. On behalf of the working group on sepsis-related problems of the European society of intensive care medicine. Intensive Care Med. 1996, 22 (7): 707-710. 10.1007/BF01709751.