Resistant starch (RS), a novel endogenous inert marker for detecting glucose absorption of small intestine with sweeteners administration in mice

Applied Biological Chemistry - 2022
Yaran Wu1, Lei Cai1, Xingzi Xie1, Shuying Yang2, Qing Shi3, Hongzhe Jia1, Xuqiang Gu1, Jingmin Deng1, Mingzhao Shi1, Qiuping Chen3, Sheng‐Li Cao3, Shuangfeng Cai2
1Food Nutrition Science Centre, Zhejiang Gongshang University, Hangzhou, 310035, People’s Republic of China
2Engineering Research Center of Molecular Medicine of Ministry of Education, Key Laboratory of Fujian Molecular Medicine, Key Laboratory of Xiamen Marine and Gene Drugs, School of Biomedical Sciences and School of Medicine, Huaqiao University, Xiamen, 361021, People’s Republic of China
3College of Biological & Environmental Sciences, Zhejiang Wanli University, Ningbo, 315100, Zhejiang, China

Tóm tắt

AbstractResistant starch could be degraded by the fermentation of colonic microorganisms in the large intestine of mammals, but not in the small intestine. In this study, we established a novel strategy by using resistant starch as an endogenous marker to determine the glucose absorption of the small intestine of laboratory animals. By optimization of the classical enzymatic method of starch measurement, the demand for the sample weight was reduced by 90%. Moreover, the amount of resistant starch in normal feed was detectable without any extra addition. The value of small intestine glucose absorption of mice was similar when using resistant starch and titanium dioxide as inert markers. The fermentation of resistant starch by intestinal microorganisms in the small intestine was demonstrated not disturbing the detection of glucose absorption significantly. Artificial sweeteners exposed ICR mice showed different glucose absorption which indicated, first, resistant starch can be used as a novel endogenous marker in the small intestine of small animals; second, although glucose tolerance did not change in mice after short-term exposure to artificial sweeteners, there were significant changes in glucose absorption associated with it; third, the short-term exposure resulted in no significant change in glucose tolerance.

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Applied Biological Chemistry

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