Resistance to Grapevine leafroll associated virus-2 is conferred by post-transcriptional gene silencing in transgenic Nicotiana benthamiana

Alkowni R, Rowhani A, Daubert S, Golino D (2004) Partial characterization of a new Ampelovirus associated with grapevine leafroll disease. J Plant Pathol 86:123–133 Batuman O, Mawassi M, Bar-Joseph M (2006) Transgene consisting of a dsRNA of an RNAi suppressor plus the 3′ UTR provide resistance to Citrus tristeza virus sequences in Nicotiana benthamiana but not in citrus. Virus Genes 33:319–327 Bucher E, Lohuis D, van Poppel PMJA, Geerts-Dimitriadou C, Goldbach R, Prins M (2006) Multiple virus resistance at a high frequency using a single transgene construct. J Gen Virol 87:3697–3701 Dolja VV, Karasev AV, Koonin EV (1994) Molecular biology and evolution of closteroviruses: sophisticated build-up of large RNA genomes. Annu Rev Phytopathol 32:261–285 Dominguez A, Guerri J, Cambra M, Navarro L, Moreno P, Pena L (2000) Efficient production of transgenic citrus plants expressing the coat protein gene of Citrus tristeza virus. Plant Cell Rep 19:427–433 Dominguez A, Hermoso de Mendoza A, Guerri J, Cambra M, Navarro L, Moreno P, Pena L (2002) Pathogen-derived resistance to Citrus tristeza virus (CTV) in transgenic Mexican lime (Citrus aurantifolia (Christ.) Swing.) plants expressing its p25 coat protein gene. Mol Breed 10:1–10 Fagoaga C, Lopez C, Moreno P, Navarro L, Flores R, Pena L (2005) Viral-like symptoms induced by the ectopic expression of the p23 gene of Citrus tristeza virus are citrus specific and do not correlate with the pathogenicity of the virus strain. Mol Plant Microbe Interact 18:435–445 Fagoaga C, Lopez C, Hermoso de Mendoza A, Moreno P, Navarro L, Flores R, Pena L (2006) Post-transcriptional gene silencing of the p23 silencing suppressor of Citrus tristeza virus confers resistance to the virus in transgenic Mexican lime. Plant Mol Biol 60:153–165 Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (2005) Virus taxonomy, eighth report of the International Committee on Taxonomy of Viruses. Academic Press, Elsevier, London, 1162 pp Febres VJ, Niblett CL, Lee RF, Moore GA (2003) Characterization of grapefruit plants (Citrus paradise Macf.) transformed with Citrus tristeza closterovirus genes. Plant Cell Rep 21:421–428 Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13 Gambino G, Gribaudo I, Leopold S, Schartl A, Laimer M (2005) Molecular characterization of grapevine plants transformed with GFLV resistance genes. I. Plant Cell Rep 24:655–662 Golles R, Moser R, Puhringer H, Katinger H, Leimer da Camara Machado M, Minafra A, Savino V, Saldarelli P, Martelli GP, Camara Machao A (2000) Transgenic grapevines expressing coat protein gene sequences of Grapevine fanleaf virus, Arabis mosaic virus, Grapevine virus A and Grapevine virus B. Acta Hortic 528:305–311 Goszczynski DE, Kasdorf GGF, Pietersen G, van Tonder H (1996) Detection of two strains of Grapevine leafroll-associated virus 2. Vitis 35:133–135 Horsch RB, Fry JE, Hoffman NL, Eichholtz D, Rogers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 277:1229–1231 Jan FJ, Fagoaga C, Pang SZ, Gonsalves D (2000) A single chimeric transgene derived from two distinct viruses coffers multi-virus resistance in transgenic plants through homology-dependent gene silencing. J Gen Virol 81:2103–2109 Krastanova S, Perrin M, Barbier P, Demangeat G, Cornuet P, Bardonnet N, Otten L, Pinck L, Walter B (1995) Transformation of grapevine rootstocks with the coat protein gene of Grapevine fanleaf nepovirus. Plant Cell Rep 14:550–554 Krastanova S, Ling KS, Zhu HY, Xue B, Burr TJ, Gonsalves D (2000) Development of transgenic grapevine rootstocks with genes from Grapevine fanleaf virus and Grapevine leafroll associated closteroviruses 2 and 3. Acta Hortic 528:367–372 Ling KS, Zhu HY, Alvizo H, Hu JS, Drong RF, Slightom JL, Gonsalves D (1997) The coat protein gene of Grapevine leafroll associated closterovirus-3: cloning, nucleotide sequencing and expression in transgenic plants. Arch Virol 142:1101–1116 Ling KS, Zhu HY, Petrovic N, Gonsalves D (2007) Serological detection of Grapevine leafroll associated virus -2 using an antiserum developed against the recombinant coat protein. J Phytopathol 155:65–69 Lu R, Folimonov A, Shintaku M, Li WX, Falk BW, Dawson WO, Ding SW (2004) Three distinct suppressors of RNA silencing encoded by a 20-kb viral RNA genome. Proc Natl Acad Sci USA 101:15742–15747 Maghuly F, Leopold S, Machado AC, Fernadez EB, Khan MA, Gambino G, Gribaudo I, Schartl A, Laimer MA (2006) Molecular characterization of grapevine plants transformed with GFLV resistance genes: II. Plant Cell Rep 25:546–553 Mauro MC, Tortain S, Walter B, Pinck L, Otten L, Coutos-Thevento P, Deloire A, Barbier P (1995) High efficiency regeneration of grapevine plants transformed with the GFLV coat protein gene. Plant Sci 112:97–106 Meng B, Li C, Goszczynski DE, Gonsalves D (2005) Genome sequences and structures of two biologically distinct strains of Grapevine leafroll-associated virus-2 and sequence analysis. Virus Genes 31:31–41 Napoli C, Lemieux C, Jorgensen R (1990) Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppression of homologous genes in trans. Plant Cell 2:279–290 Pang SZ, Jan FJ, Carney K, Stout J, Tricoli DM, Quemada HD, Gonsalves D (1996) Post-transcriptional transgene silencing and consequent Tospovirus resistance in transgenic lettuce are affected by transgene dosage and plant development. Plant J 9:899–909 Powell-Abel P, Nelson RS, De B, Hoffmann N, Rogers SG, Fraley RT, Beachy RN (1986) Delay of disease development in transgenic plants that express the Tobacco mosaic virus coat protein gene. Science 232:738–743 Prins M (2003) Broad virus resistance in transgenic plants. Trend Biotechnol 21:373–375 Quemada HD, Gonsalves D, Slightom JL (1991) Expression of coat protein gene from Cucumber mosaic virus strain C in tobacco: protection against infections by CMV strains transmitted mechanically or by aphids. Phytopathology 81:794–802 Radian-Sale S, Perl A, Edelbaum O, Kuznetsoza L, Gafny R, Sela I, Tanne E (2000) Transgenic Nicotiana benthamiana and grapevine plants transformed with Grapevine virus A (GVA) sequences. Phytoparasitica 28:1–8 Spielmann A, Krastanova S, Douet-Orhart V, Gugerli P (2000) Analysis of transgenic grapevine (Vitis rupestris) and Nicotiana benthamiana plants expressing an Arabis mosaic virus coat protein gene. Plant Sci 156:235–244 Tenllado F, Llave C, Diaz-Ruiz JR (2004) RNA interference as a new biotechnological tool for the control of virus disease in plants. Virus Res 102:85–96 Xue BD, Ling KS, Reid CL, Krastanova SV, Sekiya ME, Momol EA, Sule S, Mozsar J, Gonsalves D, Burr TJ (1999) Transformation of five grape rootstocks with plant virus genes and a virE2 gene from Agrobacterium tumefaciens. In Vitro Cell Dev Biol-Plant 35:226–231 Zhu HY, Ling KS, Goszczynski D, McFerson JR, Gonsalves D (1998) Nucleotide sequence and genome organization of Grapevine leafroll-associated virus- 2 are similar to Beet yellows virus, the Closterovirus type member. J Gen Virol 79:1289–1298