Research on Eggshell Membrane Separation for High-Purity Calcium Carbonate (CaCO3) Recovery

Engineering and Technology For Sustainable Development - Tập 34 Số 4 - Trang 1-8 - 2024
Thanh Tam Phan1, Thi Thuy Nguyen1, Hai Van Nguyen1
1School of Chemistry and Life Sciences, Hanoi University of Science and Technology, Hanoi, Vietnam

Tóm tắt

Along with growing poultry farming, a large amount of eggshell waste is generated daily from food plants and hatcheries. This waste represents a potential biological hazard and is currently being disposed of as environmental pollution. The main composition of eggshells - calcium carbonate - can produce various types of calcium products, such as calcium citrate, calcium lactate, calcium gluconate, … which are beneficial to human health. However, eggshells contain a membrane composed of proteins like collagen and sulfur-rich proteins. These membranes have a fibrous and durable structure, making them difficult to separate from the hard shell layer. The separation of eggshell membranes facilitates the production of high-purity calcium products from calcium carbonate. This study employed three methods to investigate the separation of eggshell membranes: a chemical method using NaOH (1), an enzymatic method utilizing protease enzymes (2), and a combined chemical-enzymatic treatment (3). The results showed that the composition of the eggshells was 94.63% inorganic salts and 3.58% eggshell membranes. The membrane separation efficiency reached 90.63% within 1 hour using method (1). The membrane separation efficiency was relatively high, with Alcalase 2.5L reaching 84.75% and with Protamex reaching 75.62% after 3 hours using the enzymatic method. The combined method achieved a good separation efficiency of 95.04% within 2.25 hours, and the recovered calcium purity reached 99.69%. These results demonstrate the potential for developing an industrial-scale process to treat eggshells and produce high-purity natural calcium products as a valuable source of calcium supplementation for human consumption.

Từ khóa

#Calcium carbonate #eggshell #eggshell membrane #protease enzymes

Tài liệu tham khảo

H. Chunhao, C. Yifan, S. Lei, C. Hui, L. Lanhui, N. Zhonghua, Z. Dan and W. Dehe, Advances in eggshell membrane separation and solubilization technologies, Frontiers in Veterinary Science, Sec. Animal Nutrition and Metabolism, vol. 10, Mar. 2023, pp. 1116126.

A. T. Ahmed, W. Ling, Y. Manar and H. Maxwell, Biotechnological applications of eggshell: Recent advances, Frontiers in Bioengineering and Biotechnology, vol. 9, Jul. 2021, pp. 675364.

M. Waheed, M. S. Butt, A. Shehzad, N. M. Adzahan, M.A. Shabbir, H. A. R Suleria and R. M. Aadil, Eggshell calcium: A cheap alternative to expensive supplements, Trends Food Science and Technology, vol. 91, Sep. 2019, pp. 219-230.

J. Gautron, L. Stapane, N. Le Roy, Y. Nys, A. B. Rodriguez-Navarro and M. T. Hincke, Avian eggshell Avian eggshell biomineralization: an update on its structure, mineralogy and protein tool kit, BMC Molecular and Cell Biology, vol. 22, Feb. 2021, pp. 1-17.

S. Samiullah, J.R. Roberts and K. Chousalkar, Eggshell color in brown-egg laying hens - a review, Poultry Science, vol. 94, iss. 10, Oct. 2015, pp. 2566 - 2575.

N. Laohavisuti, B. Boonchom, W. Boonmee, K. Chaiseeda, and S. Seesanong, Simple recycling of biowaste eggshells to various calcium phosphates for specific industries, Scientific reports, vol. 11, Jul. 2021, pp. 15143.

Y. Chi, Y. Wang, M. F. Li, J. Ren and Y. J. Chi, Numerical simulation and experimental study on eggshell membrane separation device, Int. J Agric. & Biol. Eng, vol 12, iss. 2, 2019, pp. 173-183.

W. Bao-huan，W. Ming-mei，Y. Na and X. Xue-ming, Enzymatic modification of eggshell membrane and characterization of its hydrolyzates, Food Science, vol. 31, no. 2, 2010, pp. 82-86.

N. H. Nurul Syazwina, A. M. Siti Azima and S. A. K. Nur Farah, Laboratory scale eggshell membrane separation and hydrolysis, Inaugural Symposium of Research and Innovation for Food (SoRIF) 2021.

T. Mark Daniel, M. K. Sean and J. V. Andrew, Method and apparatus for the enhanced separation of calcium eggshell from the organic membrane, US patent 2016/0060132 A1, 2016.

M. T. Hincke, Y. Nys and J. Gautron, The role of matrix proteins in eggshell formation, J Poultry Sci, vol. 47, iss. 3, 2010, pp. 208 - 219.

K. Mann, B. Macˇek and J. V. Olsen, Proteomic analysis of the acid-soluble organic matrix of the chicken calcified eggshell layer, Proteomics, vol. 6, iss. 13, 2006, pp. 3801 - 3810.

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Engineering and Technology For Sustainable Development

Tập 34 Số 4

1-8

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