Release of Membrane-Associated L-Dopa Decarboxylase from Human Cells

Neurochemical Research - Tập 36 - Trang 1426-1434 - 2011
Ioanna Chalatsa1, Dido Vassilacopoulou1, Emmanuel G. Fragoulis1
1Department of Biochemistry and Molecular Biology, University of Athens, Athens, Greece

Tóm tắt

L-Dopa Decarboxylase is a pyridoxal 5-phosphate (PLP)-dependent enzyme that catalyses the decarboxylation of L-Dopa to dopamine. In this study, we investigated the cellular topology of the active human enzyme. Fractionation of membranes from human cell lines, of neural and non-neural origin, by temperature-induced phase separation in Triton X-114 resulted in the detection of DDC molecules in all separation phases. Solubilization of membrane-associated DDC was observed in a pH and time-dependent manner and was affected by divalent cations and protease inhibitors, suggesting the involvement of a possible release mechanism. The study of the biological properties and function of the solubilization phenomenon described here, as well as, the study of the membrane-associated enzyme could provide us with new information about the participation of the human L-Dopa decarboxylase in physiological and aberrant processes.

Tài liệu tham khảo

Aoyagi T, Miyata S, Nanbo M et al (1969) Biological activities of leupeptins. J Antibiot Tokyo 22:558–568 Arribas J, Massague J (1995) Transforming growth factor-alpha and beta-amyloid precursor protein share a secretory mechanism. J Cell Biol 128:433–441 Batteiger B, Newhall WJT, Jones RB (1982) The use of tween 20 as a blocking agent in the immunological detection of proteins transferred to nitrocellulose membranes. J Immunol Methods 55:297–307 Baylin SB, Abeloff MD, Goodwin G et al (1980) Activities of L-dopa decarboxylase and diamine oxidase (histaminase) in human lung cancers and decarboxylase as a marker for small (oat) cell cancer in cell culture. Cancer Res 40:1990–1994 Baylin SB, Weisburger WR, Eggleston JC et al (1978) Variable content of histaminase, L-dopa decarboxylase and calcitonin in small-cell carcinoma of the lung. Biologic and clinical implications. N Engl J Med 299:105–110 Bazil V (1995) Physiological enzymatic cleavage of leukocyte membrane molecules. Immunol Today 16:135–140 Bepler G, Jaques G, Koehler A et al (1987) Markers and characteristics of human SCLC cell lines. Neuroendocrine markers, classical tumor markers, and chromosomal characteristics of permanent human small cell lung cancer cell lines. J Cancer Res Clin Oncol 113:253–259 Berry MD, Juorio AV, Li XM et al (1996) Aromatic L-amino acid decarboxylase: a neglected and misunderstood enzyme. Neurochem Res 21:1075–1087 Boomsma F, van der Hoorn FA, Schalekamp MA (1986) Determination of aromatic-L-amino acid decarboxylase in human plasma. Clin Chim Acta 159:173–183 Bordier C (1981) Phase separation of integral membrane proteins in Triton X-114 solution. J Biol Chem 256:1604–1607 Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254 Brown H (1977) In: Essman WB (ed) Serotonin-producing tumors. Serotonin in health and disease, vol 4. New York, pp 393–423 Buckland PR, Spurlock G, McGuffin P (1996) Amphetamine and vigabatrin down regulate aromatic L-amino acid decarboxylase mRNA levels. Brain Res Mol Brain Res 35:69–76 Chalatsa I, Nikolouzou E, Fragoulis EG et al (2011) L-Dopa decarboxylase expression profile in human cancer cells. Mol Biol Rep 38:1005–1011 Conway KA, Rochet J-C, Bieganski RM et al (2001) Kinetic stabilization of the alpha-Synuclein Protofibril by a Dopamine-alpha-Synuclein Adduct. Science 294:1346–1349 Davis Z, Moertel CG, McIlrath DC (1973) The malignant carcinoid syndrome. Surg Gynecol Obstet 137:637–644 Deutscher MP (1990) Maintaining protein stability. Methods Enzymol 182:83–89 Ehlers MR, Riordan JF (1991) Membrane proteins with soluble counterparts: role of proteolysis in the release of transmembrane proteins. Biochemistry 30:10065–10074 Erickson AH (1989) Biosynthesis of lysosomal endopeptidases. J Cell Biochem 40:31–41 Fragoulis EG, Sekeris CE (1975) Purification and characteristics of DOPA-decarboxylase from the integument of Calliphora vicina larve. Arch Biochem Biophys 168:15–25 Gazdar AF, Helman LJ, Israel MA et al (1988) Expression of neuroendocrine cell markers L-dopa decarboxylase, chromogranin A, and dense core granules in human tumors of endocrine and nonendocrine origin. Cancer Res 48:4078–4082 Gilbert J, Haber M, Bordow SB et al (1999) Use of tumor-specific gene expression for the differential diagnosis of neuroblastoma from other pediatric small round-cell malignancies. Am J Pathol 155:17–21 Gilbert JA, Bates LA, Ames MM (1995) Elevated aromatic-L-amino acid decarboxylase in human carcinoid tumors. Biochem Pharmacol 50:845–850 Gilbert JA, Frederick LM, Ames MM (2000) The aromatic-L-amino acid decarboxylase inhibitor carbidopa is selectively cytotoxic to human pulmonary carcinoid and small cell lung carcinoma cells. Clin Cancer Res 6:4365–4372 Hadjiconstantinou M, Wemlinger TA, Sylvia CP et al (1993) Aromatic L-amino acid decarboxylase activity of mouse striatum is modulated via dopamine receptors. J Neurochem 60:2175–2180 Hooper NM, Bashir A (1991) Glycosyl-phosphatidylinositol-anchored membrane proteins can be distinguished from transmembrane polypeptide-anchored proteins by differential solubilization and temperature-induced phase separation in Triton X-114. Biochem J 280(Pt 3):745–751 Houhou L, Lamouroux A, Biguet NF et al (1995) Expression of human dopamine beta-hydroxylase in mammalian cells infected by recombinant vaccinia virus. Mechanisms for membrane attachment. J Biol Chem 270:12601–12606 Husebye ES, Gebre-Medhin G, Tuomi T et al (1997) Autoantibodies against aromatic L-amino acid decarboxylase in autoimmune polyendocrine syndrome type I. J Clin Endocrinol Metab 82:147–150 Ichinose H, Kojima K, Togari A et al (1985) Simple purification of aromatic L-amino acid decarboxylase from human pheochromocytoma using high-performance liquid chromatography. Anal Biochem 150:408–414 Ichinose H, Ohye T, Fujita K et al (1994) Quantification of mRNA of tyrosine hydroxylase and aromatic L-amino acid decarboxylase in the substantia nigra in Parkinson’s disease and schizophrenia. J Neural Transm Park Dis Dement Sect 8:149–158 Kang UJ, Joh TH (1990) Deduced amino acid sequence of bovine aromatic L-amino acid decarboxylase: homology to other decarboxylases. Brain Res Mol Brain Res 8:83–87 Kelner KL, Morita K, Rossen JS et al (1986) Restricted diffusion of tyrosine hydroxylase and phenylethanolamine N-methyltransferase from digitonin-permeabilized adrenal chromaffin cells. Proc Natl Acad Sci USA 83:2998–3002 Krieger M, Coge F, Gros F et al (1991) Different mRNAs code for dopa decarboxylase in tissues of neuronal and nonneuronal origin. Proc Natl Acad Sci USA 88:2161–2165 Kuhn DM, Arthur R Jr, Yoon H et al (1990) Tyrosine hydroxylase in secretory granules from bovine adrenal medulla. Evidence for an integral membrane form. J Biol Chem 265:5780–5786 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685 Lloyd K, Hornykiewicz O (1970) Parkinson’s disease: activity of L-dopa decarboxylase in discrete brain regions. Science 170:1212–1213 Lutter LC, Zeichhardt H, Kurland CG (1972) Ribosomal protein neighborhoods. I. S18 and S21 as well as S5 and S8 are neighbors. Mol Gen Genet 119:357–366 Mappouras DG, Stiakakis J, Fragoulis EG (1990) Purification and characterization of L-dopa decarboxylase from human kidney. Mol Cell Biochem 94:147–156 Morita K, Teraoka K, Oka M (1987) Interaction of cytoplasmic tyrosine hydroxylase with chromaffin granule. In vitro studies on association of soluble enzyme with granule membranes and alteration in enzyme activity. J Biol Chem 262:5654–5658 Nagatsu T, Ichinose H, Kojima K et al (1985) Aromatic L-amino acid decarboxylase activities in human lung tissues: comparison between normal lung and lung carcinomas. Biochem Med 34:52–59 Nishigaki I, Ichinose H, Tamai K et al (1988) Purification of aromatic L-amino acid decarboxylase from bovine brain with a monoclonal antibody. Biochem J 252:331–335 Park JG, Oie HK, Sugarbaker PH et al (1987) Characteristics of cell lines established from human colorectal carcinoma. Cancer Res 47:6710–6718 Poulikakos P, Vassilacopoulou D, Fragoulis EG (2001) L-DOPA decarboxylase association with membranes in mouse brain. Neurochem Res 26:479–485 Poulikakos P, Vassilacopoulou D, Fragoulis EG (2002) A membrane-associated form of L-Dopa decarboxylase in the white prepuparium and adult eclosion developmental stages of the Mediterranean fruit fly, Ceratitis capitata. Biog Amines 17:15–24 Rorsman F, Husebye ES, Winqvist O et al (1995) Aromatic-L-amino-acid decarboxylase, a pyridoxal phosphate-dependent enzyme, is a beta-cell autoantigen. Proc Natl Acad Sci USA 92:8626–8629 Rossetti Z, Krajnc D, Neff NH et al (1989) Modulation of retinal aromatic L-amino acid decarboxylase via alpha 2 adrenoceptors. J Neurochem 52:647–652 Rossetti ZL, Silvia CP, Krajnc D et al (1990) Aromatic L-amino acid decarboxylase is modulated by D1 dopamine receptors in rat retina. J Neurochem 54:787–791 Sabban EL, Greene LA, Goldstein M (1983) Mechanism of biosynthesis of soluble and membrane-bound forms of dopamine beta-hydroxylase in PC12 pheochromocytoma cells. J Biol Chem 258:7812–7818 Shirota K, Fujisawa H (1988) Purification and characterization of aromatic L-amino acid decarboxylase from rat kidney and monoclonal antibody to the enzyme. J Neurochem 51:426–434 Siaterli MZ, Vassilacopoulou D, Fragoulis EG (2003) Cloning and expression of human placental L-Dopa decarboxylase. Neurochem Res 28:797–803 Sims KL, Davis GA, Bloom FE (1973) Activities of 3, 4-dihydroxy-l-phenylalanine and 5-hydroxy-l-tryptophan decarboxylases in rat brain: assay characteristics and distribution. J Neurochem 20:449–464 Springer TA (1990) Adhesion receptors of the immune system. Nature 346:425–434 Tanaka T, Horio Y, Taketoshi M et al (1989) Molecular cloning and sequencing of a cDNA of rat dopa decarboxylase: partial amino acid homologies with other enzymes synthesizing catecholamines. Proc Natl Acad Sci USA 86:8142–8146 Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354 Uccella S, Cerutti R, Vigetti D et al (2006) Histidine decarboxylase, DOPA decarboxylase, and vesicular monoamine transporter 2 expression in neuroendocrine tumors: immunohistochemical study and gene expression analysis. J Histochem Cytochem 54:863–875 Vachtenheim J, Novotna H (1997) Expression of the aromatic L-amino acid decarboxylase mRNA in human tumour cell lines of neuroendocrine and neuroectodermal origin. Eur J Cancer 33:2411–2417 Vassiliou AG, Fragoulis EG, Vassilacopoulou D (2009) Detection, purification and identification of an endogenous inhibitor of L-Dopa decarboxylase activity from human placenta. Neurochem Res 34:1089–1100 Vassiliou AG, Vassilacopoulou D, Fragoulis EG (2005) Purification of an endogenous inhibitor of L-Dopa decarboxylase activity from human serum. Neurochem Res 30:641–649 Voltattorni CB, Minelli A, Vecchini P et al (1979) Purification and characterization of 3, 4-dihydroxyphenylalanine decarboxyase from pig kidney. Eur J Biochem 93:181–188 Young EA, Neff NH, Hadjiconstantinou M (1993) Evidence for cyclic AMP-mediated increase of aromatic L-amino acid decarboxylase activity in the striatum and midbrain. J Neurochem 60:2331–2333 Young EA, Neff NH, Hadjiconstantinou M (1994) Phorbol ester administration transiently increases aromatic L-amino acid decarboxylase activity of the mouse striatum and midbrain. J Neurochem 63:694–697 Zhu MY, Juorio AV, Paterson IA et al (1992) Regulation of aromatic L-amino acid decarboxylase by dopamine receptors in the rat brain. J Neurochem 58:636–641 Zhu MY, Juorio AV, Paterson IA et al (1993) Regulation of striatal aromatic L-amino acid decarboxylase: effects of blockade or activation of dopamine receptors. Eur J Pharmacol 238:157–164