Quy định của sự co bóp cơ tim được tiết lộ bởi việc đo nồng độ Ca2+ nội bào bằng aequorin

Satoshi Kurihara1, Norio Fukuda1
1Department of Cell Physiology, The Jikei University School of Medicine, Tokyo, Japan

Tóm tắt

Trong số các ion liên quan đến chức năng cơ tim, Ca2+ là ion quan trọng nhất. Ca2+ đóng vai trò quan trọng trong quá trình cho phép cơ tim co bóp và giãn ra một cách có tổ chức; đó là quá trình được gọi là sự kết hợp kích thích – co bóp. Do đó, để có sự hiểu biết chính xác về sinh lý học của tim, việc hiểu rõ cơ chế điều chỉnh nồng độ Ca2+ nội bào để kích thích sự kết hợp kích thích – co bóp là điều cực kỳ quan trọng. Aequorin được phát hiện bởi Shimomura, Johnson và Saiga vào năm 1962. Bằng cách tận dụng thực tế rằng aequorin phát ra ánh sáng xanh khi nó liên kết với Ca2+ trong khoảng nồng độ có liên quan sinh lý, vào những năm 1970 và 1980, các nhà sinh lý học đã tiêm vi aequorin vào các mẫu cơ tim. Bằng cách này, họ đã chứng minh rằng có sự biến đổi tạm thời của Ca2+ xảy ra khi có sự khử cực màng, và sự phát triển độ căng (tức là, sự tương tác actomyosin) tiếp theo sau đó, làm tiến bộ đáng kể cho nghiên cứu về kết hợp kích thích – co bóp của tim.

Từ khóa

#Ca2+ #cơ tim #aequorin #kết hợp kích thích – co bóp #nồng độ nội bào #sinh lý học tim

Tài liệu tham khảo

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