Regulation of 11β‐HSD Genes in Human Adipose Tissue: Influence of Central Obesity and Weight Loss

Wiley - Tập 12 Số 1 - Trang 9-17 - 2004
Stefan Engeli1, Jana Böhnke2, M Feldpausch2, Kerstin Gorzelniak2, Ute Heintze2, Jürgen Janke2, Friedrich C. Luft2, Arya M. Sharma3
1HELIOS-Klinikum Berlin, Franz Volhard Clinic, Medical Faculty of the Charité, Humboldt University of Berlin, Berlin, Germany
2HELIOS‐Klinikum Berlin, Franz Volhard Clinic, Medical Faculty of the Charité, Humboldt University of Berlin and Max Delbrück Center for Molecular Medicine, Berlin, Germany
3Department of Internal Medicine, Hamilton General Hospital, McMaster University, Hamilton, Ontario, Canada

Tóm tắt

AbstractObjectives: The activity of adipose 11β‐hydroxysteroid dehydrogenase (11β‐HSD) 1 is increased in obese subjects, and animal data suggest that increased cortisol formation in adipose tissue contributes to the development of the metabolic syndrome. The aim of this study was to determine whether up‐regulation of human adipose 11β‐HSD1 in obesity can also be found at the gene expression level.Research Methods and Procedures: 11β‐HSD gene expression in subcutaneous adipose tissue biopsies of 70 postmenopausal women was studied by real‐time reverse‐transcription polymerase chain reaction. The influence of weight reduction and in vitro effects of several modulators of adipocyte gene expression on 11β‐HSD genes in human adipocytes were also studied.Results: The 11β‐HSD1 gene was highly expressed in human adipose tissue. 11β‐HSD2 mRNA was also detectable at lower levels. Adipose 11β‐HSD1 gene expression was increased by two‐fold and was positively correlated with waist circumference and homeostasis model assessment index of insulin resistance. 11β‐HSD2 gene expression was reduced by half in obese women. Weight reduction did not change gene expression levels of 11β‐HSD1 or 11β‐HSD2. Cortisol increased 11β‐HSD1 gene expression in isolated human adipocytes in vitro, whereas estradiol, triiodothyronine, angiotensin II, and pioglitazone had no influence.Discussion: Our data suggest that increased expression of the 11β‐HSD1 gene is associated with metabolic abnormalities in obese women and that increased expression of this gene may contribute to the previously reported increased local conversion of cortisone to cortisol in adipose tissue of obese individuals.

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Tài liệu tham khảo

10.1210/jc.2002-020048

10.1097/00004872-199816120-00003

10.1038/sj.ijo.0800745

10.1677/joe.0.1660145

10.1210/jcem-71-5-1215

10.1172/JCI114345

10.1016/S0022-2275(20)42044-9

10.1172/JCI116821

10.2337/diabetes.48.4.890

10.1097/00004872-200205000-00032

10.1210/jcem-67-6-1122

10.1016/S0083-6729(08)60646-9

10.1016/S1043-2760(01)00499-4

10.1016/S0140-6736(96)11222-8

10.1210/jc.83.4.1325

10.1016/S0960-0760(96)00187-2

10.1210/jc.87.6.2701

10.1046/j.1365-2265.1999.00598.x

10.1210/jc.87.7.3330

10.1210/jcem.86.3.7453

10.1126/science.1066285

10.1055/s-0029-1212128

10.3109/07435800009048591

10.1210/jc.2003-030286

10.1210/jc.2002-030017

10.1210/jc.2002-020687

10.1210/jc.87.3.1205

10.1074/jbc.M103676200

10.1210/jc.2002-021194

10.1172/JCI17845

10.1096/fj.01-0457fje

10.1210/jc.84.3.1022

10.1210/endo.142.5.8168

10.1210/jc.2002-020510

10.1210/en.140.7.3188

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Wiley

Tập 12 Số 1

9-17

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