Quan-Sheng Qiu1,2, Yan Guo1,2, Margaret A. Dietrich1,2, Karen S. Schumaker1,2, Jian‐Kang Zhu1,2
1Carnegie Institution of Washington, Stanford, CA,
2Department of Plant Sciences, University of Arizona, Tucson, AZ 85721
Tóm tắt
Maintaining low levels of sodium ions in the cell cytosol is critical for plant growth and development. Biochemical studies suggest that Na
+
/H
+
exchangers in the plasma membrane of plant cells contribute to cellular sodium homeostasis by transporting sodium ions out of the cell; however, these exchangers have not been identified at the molecular level. Genetic analysis has linked components of the
s
alt
o
verly
s
ensitive pathway (
SOS1
–
3
) to salt tolerance in
Arabidopsis thaliana
. The predicted SOS1 protein sequence and comparisons of sodium ion accumulation in wild-type and
sos1
plants suggest that SOS1 is involved directly in the transport of sodium ions across the plasma membrane. To demonstrate the transport capability of SOS1, we studied Na
+
/H
+
-exchange activity in wild-type and
sos
plants using highly purified plasma membrane vesicles. The results showed that plasma membrane Na
+
/H
+
-exchange activity was present in wild-type plants treated with 250 mM NaCl, but this transport activity was reduced by 80% in similarly treated
sos1
plants.
In vitro
addition of activated SOS2 protein (a protein kinase) increased Na
+
/H
+
-exchange activity in salt-treated wild-type plants 2-fold relative to transport without added protein. However, the addition of activated SOS2 did not have any stimulatory effect on the exchange activity in
sos1
plants. Although vesicles of
sos2
and
sos3
plants had reduced plasma membrane Na
+
/H
+
-exchange activity, transport activity in both increased with the addition of activated SOS2 protein. These results demonstrate that SOS1 contributes to plasma membrane Na
+
/H
+
exchange and that SOS2 and SOS3 regulate SOS1 transport activity.
