Regulation and functional analysis of ZmDREB2A in response to drought and heat stresses in Zea mays L

Plant Journal - Tập 50 Số 1 - Trang 54-69 - 2007
Feng Qin1, Masayuki Kakimoto2, Yoh Sakuma1,2, Kyonoshin Maruyama1, Yuriko Osakabe1,2, Lam‐Son Phan Tran1, Kazuo Shinozaki3,4, Kazuo Shinozaki1,3,2
1Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, Tsukuba, Ibaraki 305-8686, Japan
2Laboratory of Plant Molecular Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
3Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan
4RIKEN Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan, and

Tóm tắt

SummaryDREB1/CBFs and DREB2s are transcription factors that specifically interact with a cis‐acting element, DRE/CRT, which is involved in the expression of genes responsive to cold and drought stress in Arabidopsis thaliana. The function of DREB1/CBFs has been precisely analyzed and it has been found to activate the expression of many genes responsive to cold stress containing a DRE/CRT sequence in their promoters. However, the regulation and function of DREB2‐type transcription factors remained to be elucidated. In this research, we report the cloning of a DREB2 homolog from maize, ZmDREB2A, whose transcripts were accumulated by cold, dehydration, salt and heat stresses in maize seedlings. Unlike Arabidopsis DREB2A, ZmDREB2A produced two forms of transcripts, and quantitative real‐time PCR analyses demonstrated that only the functional transcription form of ZmDREB2A was significantly induced by stresses. Moreover, the ZmDREB2A protein exhibited considerably high transactivation activity compared with DREB2A in Arabidopsis protoplasts, suggesting that protein modification is not necessary for ZmDREB2A to be active. Constitutive or stress‐inducible expression of ZmDREB2A resulted in an improved drought stress tolerance in plants. Microarray analyses of transgenic plants overexpressing ZmDREB2A revealed that in addition to genes encoding late embryogenesis abundant (LEA) proteins, some genes related to heat shock and detoxification were also upregulated. Furthermore, overexpression of ZmDREB2A also enhanced thermotolerance in transgenic plants, implying that ZmDREB2A may play a dual functional role in mediating the expression of genes responsive to both water stress and heat stress.

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Tài liệu tham khảo

10.1007/BF00029852

10.1080/07352680590910410

10.1104/pp.003046

10.1105/tpc.104.026971

10.1046/j.1365-313X.2003.01661.x

10.1105/tpc.003483

10.1105/tpc.105.035659

10.1093/nar/15.21.8693

10.1023/A:1015570308704

10.1101/gad.3.3.358

10.1146/annurev.arplant.47.1.377

10.1104/pp.010548

10.1126/science.280.5360.104

10.1007/BF00049344

10.1038/7036

10.1046/j.1365-313X.2002.01325.x

10.1073/pnas.79.10.3218

10.1105/tpc.10.8.1391

10.1111/j.1365-313X.2004.02100.x

10.1093/oxfordjournals.pcp.a028913

Neill S.J., 2002, Hydrogen peroxide and nitric oxide as signaling molecules in plants, J. Exp. Bot., 53, 1237, 10.1093/jexbot/53.372.1237

10.1016/S1369-5266(02)00282-0

10.1379/1466-1268(2001)006<0177:AATHST>2.0.CO;2

10.1104/pp.001362

10.1093/pcp/pch118

10.1016/S0968-0004(96)10031-1

10.1006/bbrc.2001.6299

10.1105/tpc.105.035881

10.1073/pnas.0605639103

10.1093/pcp/pch036

10.1007/s00122-002-1131-x

10.1046/j.1365-313X.2002.01359.x

10.1007/s11103-005-2498-2

10.1073/pnas.94.3.1035

10.1104/pp.102.019398

10.1093/pcp/pce028

10.1146/annurev.arplant.50.1.571

10.1146/annurev.pp.42.060191.003051

10.1016/j.tplants.2004.03.006

10.1007/BF00039526

10.1146/annurev.cb.11.110195.002301

10.1093/nar/gnf076

10.1046/j.1365-313X.2003.01963.x

Yamaguchi‐Shinozaki K., 1994, A novel cis‐acting element in an Arabidopsis gene is involved in responsiveness to drought, low‐temperature, or high‐salt stress, Plant Cell, 6, 251

10.1016/j.tplants.2004.12.012

10.1146/annurev.arplant.57.032905.105444

10.1016/S1369-5266(00)00192-8

10.1146/annurev.arplant.53.091401.143329

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Plant Journal

Tập 50 Số 1

54-69

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