Reduction of nuclear DNA contamination in the analysis of chloroplast DNA with restriction endonucleases

Plant Molecular Biology Reporter - Tập 11 - Trang 48-53 - 1993
Jian-Wei Liu1, Ray J. Rose1
1Department of Biological Sciences, The University of Newcastle, Australia

Tóm tắt

If chloroplasts purified on sucrose step gradients are treated for 10 min at 4°C with 2 M NaCl, followed by a 1000-g centrifugation, nuclear DNA contamination is reduced 1.5 to 3 fold as estimated by densitometry.

Tài liệu tham khảo

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