Reduction of nuclear DNA contamination in the analysis of chloroplast DNA with restriction endonucleases
Tóm tắt
If chloroplasts purified on sucrose step gradients are treated for 10 min at 4°C with 2 M NaCl, followed by a 1000-g centrifugation, nuclear DNA contamination is reduced 1.5 to 3 fold as estimated by densitometry.
Tài liệu tham khảo
herrmann, R.G. 1982. The preparation of circular DNA from plastids. In:Methods in Chloroplast Molecular Biology (eds M. Edelman, R.B. Hallick and N.-H. Chua) pp. 259–280. Academic Press, San Diego.
Kemble, R.J. 1987. A rapid, single leaf, nucleic acid assay for determing the cytoplasmic organelle complement of rapeseed and relatedBrassica species. Theor. Appl. Genet. 73:364–370.
Liu, J.-W., and R.J. Rose, 1992. The spinach chloroplast chromosome is bound to the thylakoid membrane in the region of the inverted repeat. Biochem. Biophys. Res. Commun. 184:993–1000.
Palmer, J.D. 1986. Isolation and structural analysis of chloroplast DNA. In Meth. Enzymol. 118:167–186.
Palmer, J.D. 1987. Chloroplast DNA evolution and biosystematic uses of chloroplast DNA variation. Amer. Naturalist. 130:S6-S29.
Rose, R.J., L.B. Johnson, and R.J. Kemble, 1986. Restriction endonuclease studies on the chloroplast and mitochondrial DNAs of alfalfa (Medicago sativa L.). Plant Mol. Biol. 6:331–338.
Rose, R.J., S.E. Schlarbaum, E. Small, and L.B. Johnson, 1987. Chloroplast genomic variation and phylogeny inMedicago sectionIntertextae. Can. J. Bot. 66:1352–1358.
Sugiura, M. 1989. The chloroplast chromosome in land plants. Annu. Rev. Cell Biol. 5:51–70.
Svab, Z., Hajdukiewicz, P., and Maliga, P. 1990. Stable transformation of plastids in higher plants. Proc. Natl. Acad. Sci. U.S.A. 87:8526–8530.