Red blood cell acetylcholinesterase activity among healthy dwellers of an agrarian region in Sri Lanka: a descriptive cross-sectional study
Tóm tắt
Assessment of acetylcholinesterase-inhibitor insecticide (AChEII) toxicity depends on the measurement of red blood cell acetylcholinesterase (RBC-AChE) activity. Its interpretation requires baseline values which is lacking in scientific literature. We aim to find the measures of central tendency and variation for RBC-AChE activity among dwellers of Anuradhapura, where the use and abuse of AChEIIs were rampant for the last few decades. A descriptive cross-sectional study with a community-based sampling for 100 healthy non-farmers (male:female = 1:1) was done using pre-determined selection criteria. Duplicate measurements of RBC-AChE activity were performed according to the modified Ellman procedure. Pearson’s correlation and regression analysis were sort for RBC-AChE activity against its possible determinants. RBC-AChE activity had a mean of 449.8 (SD 74.2) mU/μM Hb with a statistical power of 0.847. It was similar to values of “healthy controls” from previous Sri Lankan toxicological studies but was low against international reference value [586.1 (SD 65.1) mU/μM Hb]. None of the possible determinants showed a significant strength of relationship with RBC-AChE activity. The baseline RBC-AChE activity among people of Anuradhapura is low in comparison with international reference values. This arises a need to find a causative mechanism.
Tài liệu tham khảo
Colovic MB, Krstic DZ, Lazarevic-Pasti TD, Bondzic AM, Vasic VM. Acetylcholinesterase inhibitors: pharmacology and toxicology. Curr Neuropharmacol. 2013;11(3):315–35.
Giacobini E. Cholinesterase inhibitors: new roles and therapeutic alternatives. Pharmacol Res. 2004;50(4):433–40.
Vale A, Lotti M. Organophosphorus and carbamate insecticide poisoning. Handb Clin Neurol. 2015;131:149–68.
Haboubi NA, Thurnham DI. Effect of ethanol on erythrocyte acetylcholinesterase activity. Ann Clin Biochem. 1986;23(4):458–62.
Katz KD. Organophosphate Toxicity Workup: Laboratory Studies, Imaging Studies, Electrocardiography. Medscape. 2015 [cited 2018 Feb 26]. Available from: http://emedicine.medscape.com/article/167726-workup
McNab C. WHO | Pesticides are a leading suicide method: WHO Communications; 2006. [cited 2018 Feb 4]; Available from: http://www.who.int/mediacentre/news/notes/2006/np24/en/
Thomas SHL, White J. Organophosphorus insecticides and nerve agents. In: Walker BR, Colledge NR, Ralston SH, Penman ID, editors. Davidson’s Principles and Practice of Medicine. 22nd ed. Edinburgh London New York Oxford Philadelphia St Louis Sydney Toronto: Elsevier Limited; 2014. p. 220.
Senarathna L, Jayamanna SF, Kelly PJ, Buckley NA, Dibley MJ, Dawson AH. Changing epidemiologic patterns of deliberate self poisoning in a rural district of Sri Lanka. BMC Public Health. 2012;12(1):593.
Census of Population and Housing, Department of Census and Statistics, Ministry of Policy Planning and Economic Affairs, Sri Lanka. 2012 [cited 2018 Feb 4]. p. 52. Available from: http://www.statistics.gov.lk/PopHouSat/CPH2011/Pages/Activities/Reports/FinalReport/FinalReportE.pdf.
Annual Bulletin, Sri Lanka Labour Force Survey, Department of Census and Statistics, Ministry of Finance and Planning. 2014 [cited 2018 Feb 4]. p. 2–3. Available from: http://www.statistics.gov.lk/samplesurvey/LFS_Annual Bulletin_2014-f.pdf.
Gazette [Internet]. Department of Government Printing. 2018 [cited 2018 Feb 4]. Available from: http://documents.gov.lk/en/gazette.php
Adult Tobacco Use Information. National Health Interview Survey. 2015 [cited 2017 Mar 10]. Available from: https://www.cdc.gov/nchs/nhis/tobacco/tobacco_glossary.htm
Drinking Levels Defined [Internet]. National Institute on Alcohol Abuse and Alcoholism. 2016 [cited 2017 Mar 10]. Available from: https://www.niaaa.nih.gov/alcohol-health/overview-alcohol-consumption/moderate-binge-drinking
Worek F. Standard operation procedure - Determination of cholinesterase status in whole blood and plasma. 4.1 ed. Institut für Pharmakologie und Toxikologie der Bundeswehr, München; 2013.
Worek F, Mast U, Kiderlen D, Diepold C, Eyer P. Improved determination of acetylcholinesterase activity in human whole blood. Clin Chim Acta. 1999;288(1–2):73–90.
Sigma-Aldrich, analytical reagents and solvents [Internet]. Merck KGaA, Darmstadt, Germany. 2018 [cited 2018 Feb 8]. Available from: https://www.sigmaaldrich.com/analytical-chromatography/analytical-reagents.html
AChE check Control high/low. Securetec Detektions-Systeme AG, Germany. 2018 [cited 2018 Feb 8]. Available from: https://www.securetec.net/sites/default/files/03_Produkte/ChECheck/Dateien/ache_control_acetylcholinesterase_flyer_70531_en_v01.pdf
Spectro 2000. Labomed, Inc, USA. 2001 [cited 2018 Feb 8]. Available from: http://www.labomed.com/2000rs.htm
SAS® University Edition [Internet]. SAS Institute Inc., USA. 2018 [cited 2018 Feb 8]. Available from: https://www.sas.com/en_us/software/university-edition.html
Peiris-John RJ, Ruberu DK, Wickremasinghe AR, Smit LAM, Van der Hoek W. Effects of occupational exposure to organophosphate pesticides on nerve and neuromuscular function. J Occup Environ Med. 2002;44(4):352–7.
Smit LA, et al. Neurological symptoms among Sri Lankan farmers occupationally exposed to acetyl cholinesterase-inhibiting insecticides. Am J Indian Med. 2003;44:254–64.
Peiris-John R, Wanigasuriya J, Wickremasinghe A, Dissanayake W, Hittarage A. Exposure to acetylcholinesterase-inhibiting pesticides and chronic renal failure. Ceylon Med J. 2009;51(1):42.
Test-mate ChE Cholinesterase Test System (Model 400) - Instruction Manual. [Internet]. EQM Research, Inc. Ohio; 2003 [cited 2018 Feb 4]. p. 18. Available from: http://www.eqmresearch.com/Manual-E.pdf
Arrieta DE, McCurdy SA, Henderson JD, Lefkowitz LJ, Reitstetter R, Wilson BW. Normal range of human red blood cell acetylcholinesterase activity. Drug Chem Toxicol. 2009;32(3):182–5.
Rathish D, Agampodi SB, Jayasumana MACS, Siribaddana SH. From organophosphate poisoning to diabetes mellitus: the incretin effect. Med Hypotheses. 2016;91:53–5.
Soysal P, Isik AT, Stubbs B, Solmi M, Volpe M, Luchini C, et al. Acetylcholinesterase inhibitors are associated with weight loss in older people with dementia: a systematic review and meta-analysis. J Neurol Neurosurg Psychiatry. 2016;87(12):1368–74.