Reconstitution and Functional Analysis of Kinetochore Subcomplexes

Asbury, 2006, The Dam1 kinetochore complex harnesses microtubule dynamics to produce force and movement, Proc. Natl. Acad. Sci. U.S.A., 103, 9873, 10.1073/pnas.0602249103 Berg, 1993 Cheeseman, 2006, The conserved KMN network constitutes the core microtubule-binding site of the kinetochore, Cell, 127, 983, 10.1016/j.cell.2006.09.039 Cheeseman, 2008, Molecular architecture of the kinetochore-microtubule interface, Nat. Rev. Mol. Cell Biol., 9, 33, 10.1038/nrm2310 Cras, 1999, Comparison of chemical cleaning methods of glass in preparation for silanization, Biosens. Bioelectron., 14, 683, 10.1016/S0956-5663(99)00043-3 Franck, 2007, Tension applied through the Dam1 complex promotes microtubule elongation providing a direct mechanism for length control in mitosis, Nat. Cell Biol., 9, 832, 10.1038/ncb1609 Franck, 2010, Direct physical study of kinetochore-microtubule interactions by reconstitution and interrogation with an optical force clamp, Methods, 10.1016/j.ymeth.2010.01.020 Gestaut, 2008, Phosphoregulation and depolymerization-driven movement of the Dam1 complex do not require ring formation, Nat. Cell Biol., 10, 407, 10.1038/ncb1702 Hori, 2008, CENP-O class proteins form a stable complex and are required for proper kinetochore function, Mol. Biol. Cell, 19, 843, 10.1091/mbc.E07-06-0556 Kline, 2006, The human Mis12 complex is required for kinetochore assembly and proper chromosome segregation, J. Cell Biol., 173, 9, 10.1083/jcb.200509158 Miranda, 2005, The yeast DASH complex forms closed rings on microtubules, Nat. Struct. Mol. Biol., 12, 138, 10.1038/nsmb896 Powers, 2009, The Ndc80 kinetochore complex forms load-bearing attachments to dynamic microtubule tips via biased diffusion, Cell, 136, 865, 10.1016/j.cell.2008.12.045 Rice, 1999, A structural change in the kinesin motor protein that drives motility, Nature, 402, 778, 10.1038/45483 Schoner, 1990, Enhanced translational efficiency with two-cistron expression system, Meth. Enzymol., 185, 94, 10.1016/0076-6879(90)85010-L Tan, 2001, A modular polycistronic expression system for overexpressing protein complexes in Escherichia coli, Protein Expr. Purif., 21, 224, 10.1006/prep.2000.1363 Walba, 2004, Self-assembled monolayers for liquid crystal alignment: Simple preparation on glass using alkyltrialkoxysilanes, Liq. Cryst., 31, 481, 10.1080/02678290410001666075 Wei, 2005, Molecular organization of the Ndc80 complex, an essential kinetochore component, Proc. Natl. Acad. Sci. U.S.A., 102, 5363, 10.1073/pnas.0501168102 Wei, 2007, The Ndc80/HEC1 complex is a contact point for kinetochore-microtubule attachment, Nat. Struct. Mol. Biol., 14, 54, 10.1038/nsmb1186