Reclassification of Rhizobium tropici type A strains as Rhizobium leucaenae sp. nov.

International Journal of Systematic and Evolutionary Microbiology - Tập 62 Số Pt_5 - Trang 1179-1184 - 2012
Renan Augusto Ribeiro1,2, Marco A. Rogel3, Aline López-López3, Ernesto Ormeño‐Orrillo1, Fernando Gomes Barcellos4, Julio Martínez3, Fabiano L. Thompson5, Esperanza Martínez‐Romero3, Mariangela Hungría1
1Embrapa Soja, Cx Postal 231, 86001-970 Londrina, Paraná, Brazil
2Universidade Estadual de Londrina, Department of Microbiology, Cx. Postal 60001, 86051-990 Londrina, Paraná, Brazil
3Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico
4Universidade Paranaense – UNIPAR, Cx. Postal 224, 87502-210, Umuarama, Paraná, Brazil
5UFRJ, Center of Health Sciences, Institute of Biology, Cx. Postal 68011, 21944-970, Rio de Janeiro, Brazil

Tóm tắt

Rhizobium tropici is a well-studied legume symbiont characterized by high genetic stability of the symbiotic plasmid and tolerance to tropical environmental stresses such as high temperature and low soil pH. However, high phenetic and genetic variabilities among R. tropici strains have been largely reported, with two subgroups, designated type A and B, already defined within the species. A polyphasic study comprising multilocus sequence analysis, phenotypic and genotypic characterizations, including DNA–DNA hybridization, strongly supported the reclassification of R. tropici type A strains as a novel species. Type A strains formed a well-differentiated clade that grouped with R. tropici , Rhizobium multihospitium , Rhizobium miluonense , Rhizobium lusitanum and Rhizobium rhizogenes in the phylogenies of the 16S rRNA, recA, gltA, rpoA, glnII and rpoB genes. Several phenotypic traits differentiated type A strains from all related taxa. The novel species, for which the name Rhizobium leucaenae sp. nov. is proposed, is a broad host range rhizobium being able to establish effective root-nodule symbioses with Leucaena leucocephala, Leucaena esculenta, common beans (Phaseolus vulgaris) and Gliricidia sepium. Strain CFN 299T ( = USDA 9039T = LMG 9517T = CECT 4844T = JCM 21088T = IAM 14230T = SEMIA 4083T = CENA 183T = UMR1026T = CNPSo 141T) is designated the type strain of Rhizobium leucaenae sp. nov.

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International Journal of Systematic and Evolutionary Microbiology

Tập 62 Số Pt_5

1179-1184

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